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Vol. 11, Issue 1, 39-50, January 2000

Is Phosphorylation of the alpha 1 Subunit at Ser-16 Involved in the Control of Na,K-ATPase Activity by Phorbol Ester-activated Protein Kinase C?

Eric Féraille,*dagger Dagger Pascal Béguin,dagger § Maria-Luisa Carranza,* Sandrine Gonin,* Martine Rousselot,* Pierre-Yves Martin,* Hervé Favre,* and Käthi Geering§

 *Division de Néphrologie, Hôpital Cantonal Universitaire, CH-1211 Geneva 14, Switzerland; and  §Institut de Pharmacologie et de Toxicologie de l'Université de Lausanne, CH-1005 Lausanne, Switzerland

The alpha 1 subunit of Na,K-ATPase is phosphorylated at Ser-16 by phorbol ester-sensitive protein kinase(s) C (PKC). The role of Ser-16 phosphorylation was analyzed in COS-7 cells stably expressing wild-type or mutant (T15A/S16A and S16D-E) ouabain-resistant Bufo alpha 1 subunits. In cells incubated at 37°C, phorbol 12,13-dibutyrate (PDBu) inhibited the transport activity and decreased the cell surface expression of wild-type and mutant Na,K-pumps equally (~20-30%). This effect of PDBu was mimicked by arachidonic acid and was dependent on PKC, phospholipase A2, and cytochrome P450-dependent monooxygenase. In contrast, incubation of cells at 18°C suppressed the down-regulation of Na,K-pumps and revealed a phosphorylation-dependent stimulation of the transport activity of Na,K-ATPase. Na,K-ATPase from cells expressing alpha 1-mutants mimicking Ser-16 phosphorylation (S16D or S16E) exhibited an increase in the apparent Na affinity. This finding was confirmed by the PDBu-induced increase in Na sensitivity of the activity of Na,K-ATPase measured in permeabilized nontransfected COS-7 cells. These results illustrate the complexity of the regulation of Na,K-ATPase alpha 1 isozymes by phorbol ester-sensitive PKCs and reveal 1) a phosphorylation-independent decrease in cell surface expression and 2) a phosphorylation-dependent stimulation of the transport activity attributable to an increase in the apparent Na affinity.

dagger These authors contributed equally to this work.

Dagger Corresponding author. E-mail address: feraille{at}cmu.unige.ch.

Molecular Biology of the Cell
Vol. 11, 39-50, January 2000
Copyright © 2000 by The American Society for Cell Biology


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