![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 10, 3299-3313, October 2000
and
*Departments of Medicine and Cell Biology, Washington University,
St. Louis, Missouri 63110; and Cellular adhesive events affect cell proliferation and
differentiation decisions. How cell surface events mediating adhesion transduce signals to the nucleus is not well understood. After cell-cell or cell-substratum contact, cytosolic proteins are
recruited to clustered adhesion receptor complexes. One such family of
cytosolic proteins found at sites of cell adhesion is the Zyxin family
of LIM proteins. Here we demonstrate that the family member Ajuba was
recruited to the cell surface of embryonal cells, upon aggregate formation, at sites of cell-cell contact. Ajuba contained a functional nuclear export signal and shuttled into the nucleus. Importantly, accumulation of the LIM domains of Ajuba in the nucleus of P19 embryonal cells resulted in growth inhibition and spontaneous endodermal differentiation. The differentiating effect of Ajuba mapped
to the third LIM domain, whereas regulation of proliferation mapped to
the first and second LIM domains. Ajuba-induced endodermal differentiation of these cells correlated with the capacity to activate
c-Jun kinase and required c-Jun kinase activation. These results
suggest that the cytosolic LIM protein Ajuba may provide a new
mechanism to transduce signals from sites of cell adhesion to the
nucleus, regulating cell growth and differentiation decisions during
early development.
Physiology Department,
University College London, London WC1E 6BT, England
Corresponding author. E-mail address:
longmorg{at}medicine.wustl.edu.
This article has been cited by other articles:
|
|
 |
|
  Z. Hou, H. Peng, K. Ayyanathan, K.-P. Yan, E. M. Langer, G. D. Longmore, and F. J. Rauscher III The LIM Protein AJUBA Recruits Protein Arginine Methyltransferase 5 To Mediate SNAIL-Dependent Transcriptional Repression Mol. Cell. Biol., May 15, 2008; 28(10): 3198 - 3207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Saaf, J. M. Halbleib, X. Chen, S. T. Yuen, S. Y. Leung, W. J. Nelson, and P. O. Brown Parallels between Global Transcriptional Programs of Polarizing Caco-2 Intestinal Epithelial Cells In Vitro and Gene Expression Programs in Normal Colon and Colon Cancer Mol. Biol. Cell, November 1, 2007; 18(11): 4245 - 4260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ayyanathan, H. Peng, Z. Hou, W. J. Fredericks, R. K. Goyal, E. M. Langer, G. D. Longmore, and F. J. Rauscher III The Ajuba LIM Domain Protein Is a Corepressor for SNAG Domain Mediated Repression and Participates in Nucleocytoplasmic Shuttling Cancer Res., October 1, 2007; 67(19): 9097 - 9106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Feng, H. Zhao, H. F. Luderer, H. Epple, R. Faccio, F. P. Ross, S. L. Teitelbaum, and G. D. Longmore The LIM Protein, LIMD1, Regulates AP-1 Activation through an Interaction with TRAF6 to Influence Osteoclast Development J. Biol. Chem., January 5, 2007; 282(1): 39 - 48. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wu, J. Fan, and J. G. Belasco From the Cover: MicroRNAs direct rapid deadenylation of mRNA PNAS, March 14, 2006; 103(11): 4034 - 4039. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D Heitzer and D. B. DeFranco Mechanism of Action of Hic-5/Androgen Receptor Activator 55, a LIM Domain-Containing Nuclear Receptor Coactivator Mol. Endocrinol., January 1, 2006; 20(1): 56 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Rath, Z. Wang, M. M. Lu, and E. E. Morrisey LMCD1/Dyxin Is a Novel Transcriptional Cofactor That Restricts GATA6 Function by Inhibiting DNA Binding Mol. Cell. Biol., October 15, 2005; 25(20): 8864 - 8873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rico, A. Mukherjee, M. Konieczkowski, L. A. Bruggeman, R. T. Miller, S. Khan, J. R. Schelling, and J. R. Sedor WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment Am J Physiol Renal Physiol, August 1, 2005; 289(2): F431 - F441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-J. Lai, C.-S. Chen, W.-C. Lin, and F.-T. Lin c-Src-Mediated Phosphorylation of TRIP6 Regulates Its Function in Lysophosphatidic Acid-Induced Cell Migration Mol. Cell. Biol., July 15, 2005; 25(14): 5859 - 5868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kisseleva, Y. Feng, M. Ward, C. Song, R. A. Anderson, and G. D. Longmore The LIM Protein Ajuba Regulates Phosphatidylinositol 4,5-Bisphosphate Levels in Migrating Cells through an Interaction with and Activation of PIPKI{alpha} Mol. Cell. Biol., May 15, 2005; 25(10): 3956 - 3966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Feng and G. D. Longmore The LIM Protein Ajuba Influences Interleukin-1-Induced NF-{kappa}B Activation by Affecting the Assembly and Activity of the Protein Kinase C{zeta}/p62/TRAF6 Signaling Complex Mol. Cell. Biol., May 15, 2005; 25(10): 4010 - 4022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-N. Fournier, S. Dupe-Manet, D. Bouvard, F. Luton, S. Degani, M. R. Block, S. F. Retta, and C. Albiges-Rizo Nuclear Translocation of Integrin Cytoplasmic Domain-associated Protein 1 Stimulates Cellular Proliferation Mol. Biol. Cell, April 1, 2005; 16(4): 1859 - 1871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Pratt, H. Epple, M. Ward, Y. Feng, V. M. Braga, and G. D. Longmore The LIM protein Ajuba influences p130Cas localization and Rac1 activity during cell migration J. Cell Biol., February 28, 2005; 168(5): 813 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Wu Migfilin and its binding partners: from cell biology to human diseases J. Cell Sci., February 15, 2005; 118(4): 659 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. V. Sharp, F. Munoz, D. Bourboulia, N. Presneau, E. Darai, H.-W. Wang, M. Cannon, D. N. Butcher, A. G. Nicholson, G. Klein, et al. LIM domains-containing protein 1 (LIMD1), a tumor suppressor encoded at chromosome 3p21.3, binds pRB and represses E2F-driven transcription PNAS, November 23, 2004; 101(47): 16531 - 16536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Brown and C. E. Turner Paxillin: Adapting to Change Physiol Rev, October 1, 2004; 84(4): 1315 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Srichai, M. Konieczkowski, A. Padiyar, D. J. Konieczkowski, A. Mukherjee, P. S. Hayden, S. Kamat, M. A. El-Meanawy, S. Khan, P. Mundel, et al. A WT1 Co-regulator Controls Podocyte Phenotype by Shuttling between Adhesion Structures and Nucleus J. Biol. Chem., April 2, 2004; 279(14): 14398 - 14408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Akazawa, S. Kudoh, N. Mochizuki, N. Takekoshi, H. Takano, T. Nagai, and I. Komuro A novel LIM protein Cal promotes cardiac differentiation by association with CSX/NKX2-5 J. Cell Biol., February 2, 2004; 164(3): 395 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kasai, J. Guerrero-Santoro, R. Friedman, E. S. Leman, R. H. Getzenberg, and D. B. DeFranco The Group 3 LIM Domain Protein Paxillin Potentiates Androgen Receptor Transactivation in Prostate Cancer Cell Lines Cancer Res., August 15, 2003; 63(16): 4927 - 4935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Robinson, S. Brown, M. J. McGrath, I. D. Coghill, R. Gurung, and C. A. Mitchell Skeletal muscle LIM protein 1 regulates integrin-mediated myoblast adhesion, spreading, and migration Am J Physiol Cell Physiol, March 1, 2003; 284(3): C681 - C695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. R. Petit, S. M. P. Meulemans, and W. J. M. Van de Ven The Focal Adhesion and Nuclear Targeting Capacity of the LIM-containing Lipoma-preferred Partner (LPP) Protein J. Biol. Chem., January 17, 2003; 278(4): 2157 - 2168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Marie, S. J. Pratt, M. Betson, H. Epple, J. T. Kittler, L. Meek, S. J. Moss, S. Troyanovsky, D. Attwell, G. D. Longmore, et al. The LIM Protein Ajuba Is Recruited to Cadherin-dependent Cell Junctions through an Association with alpha -Catenin J. Biol. Chem., January 3, 2003; 278(2): 1220 - 1228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Missero, M. T. Pirro, S. Simeone, M. Pischetola, and R. Di Lauro The DNA Glycosylase T:G Mismatch-specific Thymine DNA Glycosylase Represses Thyroid Transcription Factor-1-activated Transcription J. Biol. Chem., August 31, 2001; 276(36): 33569 - 33575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Aplin and R.L. Juliano Regulation of nucleocytoplasmic trafficking by cell adhesion receptors and the cytoskeleton J. Cell Biol., October 15, 2001; 155(2): 187 - 192. [Abstract] [Full Text] [PDF]
|
|
