![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 10, 3315-3327, October 2000
Departments of Biological Chemistry and Dermatology, The Johns
Hopkins University School of Medicine, Baltimore, Maryland 21205
Injury to the skin results in an induction of keratins K6, K16, and
K17 concomitant with activation of keratinocytes for
reepithelialization. Forced expression of human K16 in skin epithelia
of transgenic mice causes a phenotype that mimics several aspects of
keratinocyte activation. Two types of transgenic keratinocytes,
with forced expression of either human K16 or a K16-C14 chimeric cDNA,
were analyzed in primary culture to assess the impact of K16 expression at a cellular level. High K16-C14-expressing and low K16-expressing transgenic keratinocytes behave similar to wild type in all aspects tested. In contrast, high K16-expressing transgenic keratinocytes show
alterations in plating efficiency and calcium-induced differentiation, but proliferate normally. Migration of keratinocytes is reduced in K16
transgenic skin explants compared with controls. Finally, a subset of
high K16-expressing transgenic keratinocytes develops major changes in
the organization of keratin filaments in a time- and calcium
concentration-dependent manner. These changes coincide with alterations
in keratin content while the steady-state levels of K16 protein remain
stable. We conclude that forced expression of K16 in progenitor skin
keratinocytes directly impacts properties such as adhesion,
differentiation, and migration, and that these effects depend upon
determinants contained within its carboxy terminus.
This article has been cited by other articles:
|
|
 |
|
  X. Tong and P. A. Coulombe Keratin 17 modulates hair follicle cycling in a TNF{alpha}-dependent fashion. Genes & Dev., May 15, 2006; 20(10): 1353 - 1364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Goto, H. Sumiyoshi, T. Sakai, R. Fassler, S. Ohashi, E. Adachi, H. Yoshioka, and S. Fujiwara Elimination of Epiplakin by Gene Targeting Results in Acceleration of Keratinocyte Migration in Mice Mol. Cell. Biol., January 15, 2006; 26(2): 548 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Wong, R. Domergue, and P. A. Coulombe Overcoming Functional Redundancy To Elicit Pachyonychia Congenita-Like Nail Lesions in Transgenic Mice Mol. Cell. Biol., January 1, 2005; 25(1): 197 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Santos, A. Bravo, C. Lopez, J. M. Paramio, and J. L. Jorcano Severe Abnormalities in the Oral Mucosa Induced by Suprabasal Expression of Epidermal Keratin K10 in Transgenic Mice J. Biol. Chem., September 13, 2002; 277(38): 35371 - 35377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Santos, J. M. Paramio, A. Bravo, A. Ramirez, and J. L. Jorcano The Expression of Keratin K10 in the Basal Layer of the Epidermis Inhibits Cell Proliferation and Prevents Skin Tumorigenesis J. Biol. Chem., May 17, 2002; 277(21): 19122 - 19130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Wawersik, S. Mazzalupo, D. Nguyen, and P. A. Coulombe Increased Levels of Keratin 16 Alter Epithelialization Potential of Mouse Skin Keratinocytes In Vivo and Ex Vivo Mol. Biol. Cell, November 1, 2001; 12(11): 3439 - 3450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Bousquet, L. Ma, S. Yamada, C. Gu, T. Idei, K. Takahashi, D. Wirtz, and P. A. Coulombe The nonhelical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro J. Cell Biol., November 26, 2001; 155(5): 747 - 754. [Abstract] [Full Text] [PDF]
|
|
