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Vol. 11, Issue 10, 3495-3508, October 2000
§
and
*Harvard-Massachusetts Institute of Technology Division of Health
Sciences and Technology, Cambridge Massachusetts 02139;
Neuronal cytoskeletal elements such as neurofilaments, F-actin, and
microtubules are actively translocated by an as yet unidentified mechanism. This report describes a novel interaction between
neurofilaments and microtubule motor proteins that mediates the
translocation of neurofilaments along microtubules in vitro. Native
neurofilaments purified from spinal cord are transported along
microtubules at rates of 100-1000 nm/s to both plus and minus ends.
This motion requires ATP and is partially inhibited by vanadate,
consistent with the activity of neurofilament-bound molecular motors.
Motility is in part mediated by the dynein/dynactin motor complex and
several kinesin-like proteins. This reconstituted motile system
suggests how slow net movement of cytoskeletal polymers may be achieved by alternating activities of fast microtubule motors.
Hematology Division, Brigham and Women's Hospital,
Harvard Medical School, Boston, Massachusetts 02115; and
¶Unite Mixte de Recherche 6558 Centre National de la
Recherche Scientifique, Poitiers University, 86022 Poitiers, France
Online version of this article contains video
material for Figure 2. Online version is available at
www.molbiolcell.org.
Present address: Ludwig Institute for
Cancer Research, University of California, San Diego, La Jolla, CA 92093.
Present address: Institute for Medicine and
Engineering, University of Pennsylvania, Philadelphia, PA 19104.
§
Corresponding author: E-mail address:
flanagan{at}cnd.bwh.harvard.edu.
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