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Vol. 11, Issue 10, 3601-3615, October 2000
and
§
*Howard Hughes Medical Institute, Department of Molecular, Cellular
and Developmental Biology and During the meiotic cell cycle, a surveillance mechanism called the
"pachytene checkpoint" ensures proper chromosome segregation by
preventing meiotic progression when recombination and chromosome synapsis are defective. The silencing protein Dot1 (also known as Pch1)
is required for checkpoint-mediated pachytene arrest of the
zip1 and dmc1 mutants of
Saccharomyces cerevisiae. In the absence of
DOT1, the zip1 and dmc1
mutants inappropriately progress through meiosis, generating inviable
meiotic products. Other components of the pachytene checkpoint include
the nucleolar protein Pch2 and the heterochromatin component Sir2. In
dot1, disruption of the checkpoint correlates with the
loss of concentration of Pch2 and Sir2 in the nucleolus. In addition to
its checkpoint function, Dot1 blocks the repair of meiotic
double-strand breaks by a Rad54-dependent pathway of recombination
between sister chromatids. In vegetative cells, mutation of
DOT1 results in delocalization of Sir3 from telomeres,
accounting for the impaired telomeric silencing in dot1.
Department of Genetics,
Yale University, New Haven, Connecticut 06520-8103
Present address: Instituto de
Microbiologia-Bioquimica CSIC/Universidad de Salamanca, Spain.
§
Corresponding author: E-mail address:
shirleen.roeder{at}yale.edu.
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