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Vol. 11, Issue 10, 3645-3660, October 2000
and
*Laboratory of Molecular Biology, Department of Molecular and Cell
Biology, Graduate School of Agricultural Science, Tohoku University,
Sendai 981-8555 Japan; and Two W chromosome-linked cDNA clones, p5fm2 and p5fm3, were
obtained from a subtracted (female minus male) cDNA library prepared from a mixture of undifferentiated gonads and mesonephroi of male or
female 5-d (stages 26-28) chicken embryos. These two clones were
demonstrated to be derived from the mRNA encoding an altered form of
PKC inhibitor/interacting protein (PKCI), and its gene was named
Wpkci. The Wpkci gene reiterated ~40
times tandemly and located at the nonheterochromatic end of the chicken
W chromosome. The W linkage and the moderate reiteration of
Wpkci were conserved widely in Carinatae birds. The
chicken PKCI gene, chPKCI, was shown to
be a single-copy gene located near the centromere on the long arm of
the Z chromosome. Deduced amino acid sequences of Wpkci and chPKCI
showed ~65% identity. In the deduced sequence of Wpkci, the HIT
motif, which is essential for PKCI function, was absent, but the
Department of Molecular
Biology, Keio University School of Medicine, 35 Shinanomachi,
Shinjuku-ku, Tokyo 160, Japan
-helix region, which was conserved among the PKCI family, and a
unique Leu- and Arg-rich region, were present. Transcripts from both
Wpkci and chPKCI genes were present at
significantly higher levels in 3- to 6-d (stages 20-29) embryos. These
transcripts were detected in several embryonic tissues, including
undifferentiated left and right gonads. When the green fluorescent
protein-fused form of Wpkci was expressed in male chicken embryonic
fibroblast, it was located almost exclusively in the nucleus. A model
is presented suggesting that Wpkci may be involved in
triggering the differentiation of ovary by interfering with PKCI
function or by exhibiting its unique function in the nuclei of early
female embryos.
Corresponding author. E-mail address:
s-mizuno{at}brs.nihon-u.ac.jp.
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