![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 11, 3777-3789, November 2000
Department of Biological Chemistry and Molecular Pharmacology,
Harvard Medical School, and Department of Cancer Biology, Dana-Farber
Cancer Institute, Boston, Massachusetts 02115
In Saccharomyces cerevisiae, the 60S ribosomal
subunit assembles in the nucleolus and then is exported to the
cytoplasm, where it joins the 40S subunit for translation. Export of
the 60S subunit from the nucleus is known to be an energy-dependent and
factor-mediated process, but very little is known about the specifics
of its transport. To begin to address this problem, an assay was
developed to follow the localization of the 60S ribosomal subunit in
S. cerevisiae. Ribosomal protein L11b (Rpl11b), one of
the ~45 ribosomal proteins of the 60S subunit, was tagged at its
carboxyl terminus with the green fluorescent protein (GFP) to enable
visualization of the 60S subunit in living cells. A panel of mutant
yeast strains was screened for their accumulation of Rpl11b-GFP in the
nucleus as an indicator of their involvement in ribosome synthesis
and/or transport. This panel included conditional alleles of several rRNA-processing factors, nucleoporins, general transport factors, and
karyopherins. As predicted, conditional alleles of rRNA-processing factors that affect 60S ribosomal subunit assembly accumulated Rpl11b-GFP in the nucleus. In addition, several of the nucleoporin mutants as well as a few of the karyopherin and transport factor mutants also mislocalized Rpl11b-GFP. In particular, deletion of the
previously uncharacterized karyopherin KAP120 caused
accumulation of Rpl11b-GFP in the nucleus, whereas ribosomal protein
import was not impaired. Together, these data further define the
requirements for ribosomal subunit export and suggest a biological
function for KAP120.
Corresponding author. E-mail address:
pamela_silver{at}dfci.harvard.edu.
This article has been cited by other articles:
|
|
 |
|
  N.-J. Hung, K.-Y. Lo, S. S. Patel, K. Helmke, and A. W. Johnson Arx1 Is a Nuclear Export Receptor for the 60S Ribosomal Subunit in Yeast Mol. Biol. Cell, February 1, 2008; 19(2): 735 - 744. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Dez, M. Dlakic, and D. Tollervey Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation RNA, September 1, 2007; 13(9): 1516 - 1527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. West, J. B. Hedges, K.-Y. Lo, and A. W. Johnson Novel Interaction of the 60S Ribosomal Subunit Export Adapter Nmd3 at the Nuclear Pore Complex J. Biol. Chem., May 11, 2007; 282(19): 14028 - 14037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Oeffinger, A. Fatica, M. P. Rout, and D. Tollervey Yeast Rrp14p is required for ribosomal subunit synthesis and for correct positioning of the mitotic spindle during mitosis Nucleic Acids Res., February 28, 2007; 35(4): 1354 - 1366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Seiser, A. E. Sundberg, B. J. Wollam, P. Zobel-Thropp, K. Baldwin, M. D. Spector, and D. E. Lycan Ltv1 Is Required for Efficient Nuclear Export of the Ribosomal Small Subunit in Saccharomyces cerevisiae Genetics, October 1, 2006; 174(2): 679 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Q. Ni, L.-P. Liu, D. Hess, J. Rietdorf, and F.-L. Sun Drosophila ribosomal proteins are associated with linker histone H1 and suppress gene transcription Genes & Dev., July 15, 2006; 20(14): 1959 - 1973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bakhrat, K. Baranes, O. Krichevsky, I. Rom, G. Schlenstedt, S. Pietrokovski, and D. Raveh Nuclear Import of Ho Endonuclease Utilizes Two Nuclear Localization Signals and Four Importins of the Ribosomal Import System J. Biol. Chem., May 5, 2006; 281(18): 12218 - 12226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Caesar, M. Greiner, and G. Schlenstedt Kap120 Functions as a Nuclear Import Receptor for Ribosome Assembly Factor Rpf1 in Yeast Mol. Cell. Biol., April 15, 2006; 26(8): 3170 - 3180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Butterfield-Gerson, L. Z. Scheifele, E. P. Ryan, A. K. Hopper, and L. J. Parent Importin-{beta} Family Members Mediate Alpharetrovirus Gag Nuclear Entry via Interactions with Matrix and Nucleocapsid J. Virol., February 15, 2006; 80(4): 1798 - 1806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Du, M. R.K. S. Rao, X. Q. Chen, W. Wu, S. Mahalingam, and D. Balasundaram The Homologous Putative GTPases Grn1p from Fission Yeast and the Human GNL3L Are Required for Growth and Play a Role in Processing of Nucleolar Pre-rRNA Mol. Biol. Cell, January 1, 2006; 17(1): 460 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dong, R. Lai, J. L. Jennings, A. J. Link, and A. G. Hinnebusch The Novel ATP-Binding Cassette Protein ARB1 Is a Shuttling Factor That Stimulates 40S and 60S Ribosome Biogenesis Mol. Cell. Biol., November 15, 2005; 25(22): 9859 - 9873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. THOMSON and D. TOLLERVEY Nop53p is required for late 60S ribosome subunit maturation and nuclear export in yeast RNA, August 1, 2005; 11(8): 1215 - 1224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. West, J. B. Hedges, A. Chen, and A. W. Johnson Defining the Order in Which Nmd3p and Rpl10p Load onto Nascent 60S Ribosomal Subunits Mol. Cell. Biol., May 1, 2005; 25(9): 3802 - 3813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Irwin, M. Aye, P. Baldi, N. Beliakova-Bethell, H. Cheng, Y. Dou, W. Liou, and S. Sandmeyer Retroviruses and yeast retrotransposons use overlapping sets of host genes Genome Res., May 1, 2005; 15(5): 641 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Shiina, K. Shinkura, and M. Tokunaga A Novel RNA-Binding Protein in Neuronal RNA Granules: Regulatory Machinery for Local Translation J. Neurosci., April 27, 2005; 25(17): 4420 - 4434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. BRUNE, S. E. MUNCHEL, N. FISCHER, A. V. PODTELEJNIKOV, and K. WEIS Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export RNA, April 1, 2005; 11(4): 517 - 531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schaper, J. Franke, S. H. Meijsing, and A. E. Ehrenhofer-Murray Nuclear import of the histone acetyltransferase complex SAS-I in Saccharomyces cerevisiae J. Cell Sci., April 1, 2005; 118(7): 1473 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Saiardi, R. Bhandari, A. C. Resnick, A. M. Snowman, and S. H. Snyder Phosphorylation of Proteins by Inositol Pyrophosphates Science, December 17, 2004; 306(5704): 2101 - 2105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Belanger, L. A. Simmons, J. K. Roth, K. A. VanderPloeg, L. B. Lichten, and B. Fahrenkrog The Karyopherin Msn5/Kap142 Requires Nup82 for Nuclear Export and Performs a Function Distinct from Translocation in RPA Protein Import J. Biol. Chem., October 15, 2004; 279(42): 43530 - 43539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dong, R. Lai, K. Nielsen, C. A. Fekete, H. Qiu, and A. G. Hinnebusch The Essential ATP-binding Cassette Protein RLI1 Functions in Translation by Promoting Preinitiation Complex Assembly J. Biol. Chem., October 1, 2004; 279(40): 42157 - 42168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Oeffinger, M. Dlakic, and D. Tollervey A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits Genes & Dev., January 15, 2004; 18(2): 196 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. FATICA, M. OEFFINGER, D. TOLLERVEY, and I. BOZZONI Cic1p/Nsa3p is required for synthesis and nuclear export of 60S ribosomal subunits RNA, December 1, 2003; 9(12): 1431 - 1436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Fingerman, V. Nagaraj, D. Norris, and A. K. Vershon Sfp1 Plays a Key Role in Yeast Ribosome Biogenesis Eukaryot. Cell, October 1, 2003; 2(5): 1061 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kallstrom, J. Hedges, and A. Johnson The Putative GTPases Nog1p and Lsg1p Are Required for 60S Ribosomal Subunit Biogenesis and Are Localized to the Nucleus and Cytoplasm, Respectively Mol. Cell. Biol., June 15, 2003; 23(12): 4344 - 4355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Thomas and U. Kutay Biogenesis and nuclear export of ribosomal subunits in higher eukaryotes depend on the CRM1 export pathway J. Cell Sci., June 15, 2003; 116(12): 2409 - 2419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Nikolaev, M.-F. Cochet, and B. Felenbok Nuclear Import of Zinc Binuclear Cluster Proteins Proceeds through Multiple, Overlapping Transport Pathways Eukaryot. Cell, April 1, 2003; 2(2): 209 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sydorskyy, D. J. Dilworth, E. C. Yi, D. R. Goodlett, R. W. Wozniak, and J. D. Aitchison Intersection of the Kap123p-Mediated Nuclear Import and Ribosome Export Pathways Mol. Cell. Biol., March 15, 2003; 23(6): 2042 - 2054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. P. Lei, C. A. Stern, B. Fahrenkrog, H. Krebber, T. I. Moy, U. Aebi, and P. A. Silver Sac3 Is an mRNA Export Factor That Localizes to Cytoplasmic Fibrils of Nuclear Pore Complex Mol. Biol. Cell, March 1, 2003; 14(3): 836 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhao, J.-H. Sohn, and J. R. Warner Autoregulation in the Biosynthesis of Ribosomes Mol. Cell. Biol., January 15, 2003; 23(2): 699 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bjork, G. Bauren, S. Jin, Y.-G. Tong, T. R. Burglin, U. Hellman, and L. Wieslander A Novel Conserved RNA-binding Domain Protein, RBD-1, Is Essential For Ribosome Biogenesis Mol. Biol. Cell, October 1, 2002; 13(10): 3683 - 3695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Ferrezuelo, B. Steiner, M. Aldea, and B. Futcher Biogenesis of Yeast Telomerase Depends on the Importin Mtr10 Mol. Cell. Biol., September 1, 2002; 22(17): 6046 - 6055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. I. Moy and P. A. Silver Requirements for the nuclear export of the small ribosomal subunit J. Cell Sci., July 15, 2002; 115(14): 2985 - 2995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-B. Jin, J. Zhao, P. Bjork, K. Schmekel, Per. O. Ljungdahl, and L. Wieslander Mrd1p Is Required for Processing of Pre-rRNA and for Maintenance of Steady-state Levels of 40 S Ribosomal Subunits in Yeast J. Biol. Chem., May 17, 2002; 277(21): 18431 - 18439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Franke, B. Reimann, E. Hartmann, M. Kohler, and B. Wiedmann Evidence for a nuclear passage of nascent polypeptide-associated complex subunits in yeast J. Cell Sci., March 9, 2002; 114(14): 2641 - 2648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Plafker and I. G. Macara Ribosomal Protein L12 Uses a Distinct Nuclear Import Pathway Mediated by Importin 11 Mol. Cell. Biol., February 15, 2002; 22(4): 1266 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Valentini, J. M. Casolari, C. C. Oliveira, P. A. Silver, and A. E. McBride Genetic Interactions of Yeast Eukaryotic Translation Initiation Factor 5A (eIF5A) Reveal Connections to Poly(A)-Binding Protein and Protein Kinase C Signaling Genetics, February 1, 2002; 160(2): 393 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.-E. Gleizes, J. Noaillac-Depeyre, I. Leger-Silvestre, F. Teulieres, J.-Y. Dauxois, D. Pommet, M.-C. Azum-Gelade, and N. Gas Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex J. Cell Biol., December 10, 2001; 155(6): 923 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. G. Macara Transport into and out of the Nucleus Microbiol. Mol. Biol. Rev., December 1, 2001; 65(4): 570 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. P. C. Allen, L. Huang, A. Burlingame, and M. Rexach Proteomic Analysis of Nucleoporin Interacting Proteins J. Biol. Chem., July 27, 2001; 276(31): 29268 - 29274. [Abstract] [Full Text] [PDF]
|
|
