Molecular Biology of the Cell click for ASCB 2009 Annual Meeting page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boylan, K.
Right arrow Articles by Hays, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boylan, K.
Right arrow Articles by Hays, T.

Vol. 11, Issue 11, 3791-3803, November 2000

A Molecular Genetic Analysis of the Interaction between the Cytoplasmic Dynein Intermediate Chain and the Glued (Dynactin) Complex

Kristin Boylan,* Madeline Serr, and Tom Hays

Department of Genetics, Cell Biology, and Development, University of Minnesota, St. Paul, Minnesota 55108

The microtubule motor cytoplasmic dynein performs multiple cellular functions; however, the regulation and targeting of the motor to different cargoes is not well understood. A biochemical interaction between the dynein intermediate chain subunit and the p150-Glued component of the dynein regulatory complex, dynactin, has supported the hypothesis that the intermediate chain is a key modulator of dynein attachment to cellular cargoes. In this report, we identify multiple intermediate chain polypeptides that cosediment with the 19S dynein complex and two differentially expressed transcripts derived from the single cytoplasmic dynein intermediate chain (Cdic) gene that differ in the 3' untranslated region sequence. These results support previous observations of multiple Cdic gene products that may contribute to the specialization of dynein function. Most significantly, we provide genetic evidence that the interaction between the dynein intermediate chain and p150-Glued is functionally relevant. We use a genomic Cdic transgene to show that extra copies of the dynein intermediate chain gene act to suppress the rough eye phenotype of the mutant Glued1, a mutation in the p150-Glued subunit of dynactin. Furthermore, we show that the interaction between the dynein intermediate chain and p150-Glued is dependent on the dosage of the Cdic gene. This result suggests that the dynein intermediate chain may be a limiting component in the assembly of the dynein complex and that the regulation of the interaction between the dynein intermediate chain and dynactin is critical for dynein function.

* Corresponding author. E-mail address: kboylan{at}biosci.cbs.umn.edu.

Molecular Biology of the Cell
Vol. 11, 3791-3803, November 2000
Copyright © 2000 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
S. Mische, Y. He, L. Ma, M. Li, M. Serr, and T. S. Hays
Dynein Light Intermediate Chain: An Essential Subunit That Contributes to Spindle Checkpoint Inactivation
Mol. Biol. Cell, November 1, 2008; 19(11): 4918 - 4929.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
B. Riggs, B. Fasulo, A. Royou, S. Mische, J. Cao, T. S. Hays, and W. Sullivan
The Concentration of Nuf, a Rab11 Effector, at the Microtubule-organizing Center Is Cell Cycle regulated, Dynein-dependent, and Coincides with Furrow Formation
Mol. Biol. Cell, September 1, 2007; 18(9): 3313 - 3322.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Mische, M. Li, M. Serr, and T. S. Hays
Direct Observation of Regulated Ribonucleoprotein Transport Across the Nurse Cell/Oocyte Boundary
Mol. Biol. Cell, June 1, 2007; 18(6): 2254 - 2263.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. W.-H. Lo, H.-M. Kan, and K. K. Pfister
Identification of a Novel Region of the Cytoplasmic Dynein Intermediate Chain Important for Dimerization in the Absence of the Light Chains
J. Biol. Chem., April 7, 2006; 281(14): 9552 - 9559.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Iwasaki, T. Kuwata, Y. Yamazaki, N. A. Jenkins, N. G. Copeland, M. Osato, Y. Ito, E. Kroon, G. Sauvageau, and T. Nakamura
Identification of cooperative genes for NUP98-HOXA9 in myeloid leukemogenesis using a mouse model
Blood, January 15, 2005; 105(2): 784 - 793.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M.-g. Li, M. Serr, E. A. Newman, and T. S. Hays
The Drosophila tctex-1 Light Chain Is Dispensable for Essential Cytoplasmic Dynein Functions but Is Required during Spermatid Differentiation
Mol. Biol. Cell, July 1, 2004; 15(7): 3005 - 3014.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. J. King, C. L. Brown, K. C. Maier, N. J. Quintyne, and T. A. Schroer
Analysis of the Dynein-Dynactin Interaction In Vitro and In Vivo
Mol. Biol. Cell, December 1, 2003; 14(12): 5089 - 5097.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Suikkanen, T. Aaltonen, M. Nevalainen, O. Valilehto, L. Lindholm, M. Vuento, and M. Vihinen-Ranta
Exploitation of Microtubule Cytoskeleton and Dynein during Parvoviral Traffic toward the Nucleus
J. Virol., October 1, 2003; 77(19): 10270 - 10279.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. L. M. Boylan and T. S. Hays
The Gene for the Intermediate Chain Subunit of Cytoplasmic Dynein Is Essential in Drosophila
Genetics, November 1, 2002; 162(3): 1211 - 1220.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]