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Vol. 11, Issue 11, 3873-3883, November 2000
and
Departments of *Anatomy and To determine the distribution of the epidermal growth factor (EGF)
receptor (EGFR) on the surface of cells responding to EGF as a
chemoattractant, an EGFR-green fluorescent protein chimera was
expressed in the MTLn3 mammary carcinoma cell line. The chimera was
functional and easily visualized on the cell surface. In contrast to
other studies indicating that the EGFR might be localized to certain
regions of the plasma membrane, we found that the chimera is
homogeneously distributed on the plasma membrane and becomes most
concentrated in vesicles after endocytosis. In spatial gradients of
EGF, endocytosed receptor accumulates on the upgradient side of the
cell. Visualization of the binding of fluorescent EGF to cells reveals
that the affinity properties of the receptor, together with its
expression level on cells, can provide an initial amplification step in
spatial gradient sensing.
Developmental
and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY
10461
Online version of this article contains video material to
accompany Figures 1-6. Online version is available at
www.molbiolcell.org
Corresponding author. E-mail address:
segall{at}aecom.yu.edu
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