![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 11, 3873-3883, November 2000
and
Departments of *Anatomy and To determine the distribution of the epidermal growth factor (EGF)
receptor (EGFR) on the surface of cells responding to EGF as a
chemoattractant, an EGFR-green fluorescent protein chimera was
expressed in the MTLn3 mammary carcinoma cell line. The chimera was
functional and easily visualized on the cell surface. In contrast to
other studies indicating that the EGFR might be localized to certain
regions of the plasma membrane, we found that the chimera is
homogeneously distributed on the plasma membrane and becomes most
concentrated in vesicles after endocytosis. In spatial gradients of
EGF, endocytosed receptor accumulates on the upgradient side of the
cell. Visualization of the binding of fluorescent EGF to cells reveals
that the affinity properties of the receptor, together with its
expression level on cells, can provide an initial amplification step in
spatial gradient sensing.
Developmental
and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY
10461
Online version of this article contains video material to
accompany Figures 1-6. Online version is available at
www.molbiolcell.org
Corresponding author. E-mail address:
segall{at}aecom.yu.edu
This article has been cited by other articles:
|
|
 |
|
  J. Monypenny, D. Zicha, C. Higashida, F. Oceguera-Yanez, S. Narumiya, and N. Watanabe Cdc42 and Rac Family GTPases Regulate Mode and Speed but Not Direction of Primary Fibroblast Migration during Platelet-Derived Growth Factor-Dependent Chemotaxis Mol. Cell. Biol., May 15, 2009; 29(10): 2730 - 2747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Z. Rappoport and S. M. Simon Endocytic trafficking of activated EGFR is AP-2 dependent and occurs through preformed clathrin spots J. Cell Sci., May 1, 2009; 122(9): 1301 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ghosh, M. Garcia-Marcos, S. J. Bornheimer, and M. G. Farquhar Activation of G{alpha}i3 triggers cell migration via regulation of GIV J. Cell Biol., July 28, 2008; 182(2): 381 - 393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pu, C. D. McCaig, L. Cao, Z. Zhao, J. E. Segall, and M. Zhao EGF receptor signalling is essential for electric-field-directed migration of breast cancer cells J. Cell Sci., October 1, 2007; 120(19): 3395 - 3403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gampel, L. Moss, M. C. Jones, V. Brunton, J. C. Norman, and H. Mellor VEGF regulates the mobilization of VEGFR2/KDR from an intracellular endothelial storage compartment Blood, October 15, 2006; 108(8): 2624 - 2631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Orth, E. W. Krueger, S. G. Weller, and M. A. McNiven A novel endocytic mechanism of epidermal growth factor receptor sequestration and internalization. Cancer Res., April 1, 2006; 66(7): 3603 - 3610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Xue, J. Wyckoff, F. Liang, M. Sidani, S. Violini, K.-L. Tsai, Z.-Y. Zhang, E. Sahai, J. Condeelis, and J. E. Segall Epidermal Growth Factor Receptor Overexpression Results in Increased Tumor Cell Motility In vivo Coordinately with Enhanced Intravasation and Metastasis Cancer Res., January 1, 2006; 66(1): 192 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-X. Zhu, S. Goldoni, G. Bix, R. T. Owens, D. J. McQuillan, C. C. Reed, and R. V. Iozzo Decorin Evokes Protracted Internalization and Degradation of the Epidermal Growth Factor Receptor via Caveolar Endocytosis J. Biol. Chem., September 16, 2005; 280(37): 32468 - 32479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. McCaig, A. M. Rajnicek, B. Song, and M. Zhao Controlling Cell Behavior Electrically: Current Views and Future Potential Physiol Rev, July 1, 2005; 85(3): 943 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Hunton, W. G. Barnes, J. Kim, X.-R. Ren, J. D. Violin, E. Reiter, G. Milligan, D. D. Patel, and R. J. Lefkowitz {beta}-Arrestin 2-Dependent Angiotensin II Type 1A Receptor-Mediated Pathway of Chemotaxis Mol. Pharmacol., April 1, 2005; 67(4): 1229 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gallio, C. Englund, P. Kylsten, and C. Samakovlis Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline Development, August 1, 2004; 131(15): 3605 - 3614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Kempiak, S.-C. Yip, J. M. Backer, and J. E. Segall Local signaling by the EGF receptor J. Cell Biol., September 1, 2003; 162(5): 781 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Neumeister, F. J. Pixley, Y. Xiong, H. Xie, K. Wu, A. Ashton, M. Cammer, A. Chan, M. Symons, E. R. Stanley, et al. Cyclin D1 Governs Adhesion and Motility of Macrophages Mol. Biol. Cell, May 1, 2003; 14(5): 2005 - 2015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Denker and D. L. Barber Cell migration requires both ion translocation and cytoskeletal anchoring by the Na-H exchanger NHE1 J. Cell Biol., December 23, 2002; 159(6): 1087 - 1096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Parsons, M. D. Keppler, A. Kline, A. Messent, M. J. Humphries, R. Gilchrist, I. R. Hart, C. Quittau-Prevostel, W. E. Hughes, P. J. Parker, et al. Site-Directed Perturbation of Protein Kinase C- Integrin Interaction Blocks Carcinoma Cell Chemotaxis Mol. Cell. Biol., August 15, 2002; 22(16): 5897 - 5911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Caric, H. Raphael, J. Viti, A. Feathers, D. Wancio, and L. Lillien EGFRs mediate chemotactic migration in the developing telencephalon Development, November 1, 2001; 128(21): 4203 - 4216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Ridley Rho GTPases and cell migration J. Cell Sci., January 8, 2001; 114(15): 2713 - 2722. [Abstract] [Full Text] [PDF]
|
|
