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Vol. 11, Issue 11, 3885-3896, November 2000

The Yeast Nucleoporin Nup53p Specifically Interacts with Nic96p and Is Directly Involved in Nuclear Protein Import

Birthe Fahrenkrog,^* Wolfgang Hübner, Anna Mandinova,^* Nelly Panté,^§ Walter Keller, and Ueli Aebi^*¶

 ^*M. E. Müller Institute for Structural Biology, and  Department of Cell Biology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland; and  ^§Institute of Biochemistry, Federal Institute of Technology Zürich (ETHZ), CH-8092 Zürich, Switzerland

The bidirectional nucleocytoplasmic transport of macromolecules is mediated by the nuclear pore complex (NPC) which, in yeast, is composed of ~30 different proteins (nucleoporins). Pre-embedding immunogold-electron microscopy revealed that Nic96p, an essential yeast nucleoporin, is located about the cytoplasmic and the nuclear periphery of the central channel, and near or at the distal ring of the yeast NPC. Genetic approaches further implicated Nic96p in nuclear protein import. To more specifically explore the potential role of Nic96p in nuclear protein import, we performed a two-hybrid screen with NIC96 as the bait against a yeast genomic library to identify transport factors and/or nucleoporins involved in nuclear protein import interacting with Nic96p. By doing so, we identified the yeast nucleoporin Nup53p, which also exhibits multiple locations within the yeast NPC and colocalizes with Nic96p in all its locations. Whereas Nup53p is directly involved in NLS-mediated protein import by its interaction with the yeast nuclear import receptor Kap95p, it appears not to participate in NES-dependent nuclear export.

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Present addresses: European Molecular Biology Laboratory, Meyerhofstr.1, D-69117 Heidelberg, Germany; Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.

^¶ Corresponding author. E-mail address: Birthe.Fahrenkrog{at}embl-heidelberg.de.

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Molecular Biology of the Cell
Vol. 11, 3885-3896, November 2000
Copyright © 2000 by The American Society for Cell Biology

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