![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 11, 3963-3976, November 2000
Department of Biochemistry, University of Amsterdam, Academic
Medical Center, Meibergdreef 15 1105 AZ Amsterdam, The Netherlands
A number of peroxisome-associated proteins have been described that
are involved in the import of proteins into peroxisomes, among which is
the receptor for peroxisomal targeting signal 1 (PTS1) proteins
Pex5p, the integral membrane protein Pex13p, which contains an Src
homology 3 (SH3) domain, and the peripheral membrane protein Pex14p. In
the yeast Saccharomyces cerevisiae, both Pex5p and
Pex14p are able to bind Pex13p via its SH3 domain. Pex14p contains the
classical SH3 binding motif PXXP, whereas this sequence is absent in
Pex5p. Mutation of the conserved tryptophan in the PXXP binding pocket
of Pex13-SH3 abolished interaction with Pex14p, but did not affect
interaction with Pex5p, suggesting that Pex14p is the classical SH3
domain ligand and that Pex5p binds the SH3 domain in an alternative
way. To identify the SH3 binding site in Pex5p, we screened a randomly
mutagenized PEX5 library for loss of interaction with
Pex13-SH3. Such mutations were all located in a small region in the
N-terminal half of Pex5p. One of the altered residues (F208) was part
of the sequence W204XXQF208, that is conserved
between Pex5 proteins of different species. Site-directed mutagenesis
of Trp204 confirmed the essential role of this motif in recognition of
the SH3 domain. The Pex5p mutants could only partially restore
PTS1-protein import in pex5
cells in vivo. In vitro
binding studies showed that these Pex5p mutants failed to interact with
Pex13-SH3 in the absence of Pex14p, but regained their ability to bind
in the presence of Pex14p, suggesting the formation of a heterotrimeric
complex consisting of Pex5p, Pex14p, and Pex13-SH3. In vivo, these
Pex5p mutants, like wild-type Pex5p, were still found to be associated
with peroxisomes. Taken together, this indicates that in the absence of
Pex13-SH3 interaction, other protein(s) is able to bind Pex5p at the
peroxisome; Pex14p is a likely candidate for this function.
This article has been cited by other articles:
|
|
 |
|
  A. L. Samuels, S. P. Klinken, and E. Ingley Liar, a novel Lyn-binding nuclear/cytoplasmic shuttling protein that influences erythropoietin-induced differentiation Blood, April 16, 2009; 113(16): 3845 - 3856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Piekarska, G. Hardy, E. Mol, J. van den Burg, K. Strijbis, C. van Roermund, M. van den Berg, and B. Distel The activity of the glyoxylate cycle in peroxisomes of Candida albicans depends on a functional {beta}-oxidation pathway: evidence for reduced metabolite transport across the peroxisomal membrane Microbiology, October 1, 2008; 154(10): 3061 - 3072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Oshima, A. Kamigaki, C. Nakamori, S. Mano, M. Hayashi, M. Nishimura, and M. Esaka Plant Catalase is Imported into Peroxisomes by Pex5p but is Distinct from Typical PTS1 Import Plant Cell Physiol., April 1, 2008; 49(4): 671 - 677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Williams, M. van den Berg, R. R. Sprenger, and B. Distel A Conserved Cysteine Is Essential for Pex4p-dependent Ubiquitination of the Peroxisomal Import Receptor Pex5p J. Biol. Chem., August 3, 2007; 282(31): 22534 - 22543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wu, C. Ji, F. Xie, C. D. Langefeld, K. Qian, A. W. Gibson, J. C. Edberg, and R. P. Kimberly Fc{alpha}RI (CD89) Alleles Determine the Proinflammatory Potential of Serum IgA J. Immunol., March 15, 2007; 178(6): 3973 - 3982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ma, J. G. Forbes, G. Gutierrez-Cruz, and K. Wang Titin as a Giant Scaffold for Integrating Stress and Src Homology Domain 3-mediated Signaling Pathways: THE CLUSTERING OF NOVEL OVERLAP LIGAND MOTIFS IN THE ELASTIC PEVK SEGMENT J. Biol. Chem., September 15, 2006; 281(37): 27539 - 27556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Miyata and Y. Fujiki Shuttling Mechanism of Peroxisome Targeting Signal Type 1 Receptor Pex5: ATP-Independent Import and ATP-Dependent Export Mol. Cell. Biol., December 15, 2005; 25(24): 10822 - 10832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kragt, T. Voorn-Brouwer, M. van den Berg, and B. Distel Endoplasmic Reticulum-directed Pex3p Routes to Peroxisomes and Restores Peroxisome Formation in a Saccharomyces cerevisiae pex3{Delta} Strain J. Biol. Chem., October 7, 2005; 280(40): 34350 - 34357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schell-Steven, K. Stein, M. Amoros, C. Landgraf, R. Volkmer-Engert, H. Rottensteiner, and R. Erdmann Identification of a Novel, Intraperoxisomal Pex14-Binding Site in Pex13: Association of Pex13 with the Docking Complex Is Essential for Peroxisomal Matrix Protein Import Mol. Cell. Biol., April 15, 2005; 25(8): 3007 - 3018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kragt, T. Voorn-Brouwer, M. van den Berg, and B. Distel The Saccharomyces cerevisiae Peroxisomal Import Receptor Pex5p Is Monoubiquitinated in Wild Type Cells J. Biol. Chem., March 4, 2005; 280(9): 7867 - 7874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schafer, D. Kerssen, M. Veenhuis, W.-H. Kunau, and W. Schliebs Functional Similarity between the Peroxisomal PTS2 Receptor Binding Protein Pex18p and the N-Terminal Half of the PTS1 Receptor Pex5p Mol. Cell. Biol., October 15, 2004; 24(20): 8895 - 8906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Madrid, G. De Crescenzo, S. Wang, and A. Jardim Modulation of the Leishmania donovani Peroxin 5 Quaternary Structure by Peroxisomal Targeting Signal 1 Ligands Mol. Cell. Biol., September 1, 2004; 24(17): 7331 - 7344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. de Graaf, W. T. Zwart, R. A.J. van Dijken, M. Deneka, T. K.F. Schulz, N. Geijsen, P. J. Coffer, B. M. Gadella, A. J. Verkleij, P. van der Sluijs, et al. Phosphatidylinositol 4-Kinase{beta} Is Critical for Functional Association of rab11 with the Golgi Complex Mol. Biol. Cell, April 1, 2004; 15(4): 2038 - 2047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Birschmann, A. K. Stroobants, M. van den Berg, A. Schafer, K. Rosenkranz, W.-H. Kunau, and H. F. Tabak Pex15p of Saccharomyces cerevisiae Provides a Molecular Basis for Recruitment of the AAA Peroxin Pex6p to Peroxisomal Membranes Mol. Biol. Cell, June 1, 2003; 14(6): 2226 - 2236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S. Oh, H. Kwon, S. K. Sun, and C.-H. Yang QM, a Putative Tumor Suppressor, Regulates Proto-oncogene c-Yes J. Biol. Chem., September 20, 2002; 277(39): 36489 - 36498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. J. Klein, M. van den Berg, G. Bottger, H. F. Tabak, and B. Distel Saccharomyces cerevisiae Acyl-CoA Oxidase Follows a Novel, Non-PTS1, Import Pathway into Peroxisomes That Is Dependent on Pex5p J. Biol. Chem., July 5, 2002; 277(28): 25011 - 25019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Otera, K. Setoguchi, M. Hamasaki, T. Kumashiro, N. Shimizu, and Y. Fujiki Peroxisomal Targeting Signal Receptor Pex5p Interacts with Cargoes and Import Machinery Components in a Spatiotemporally Differentiated Manner: Conserved Pex5p WXXXF/Y Motifs Are Critical for Matrix Protein Import Mol. Cell. Biol., March 15, 2002; 22(6): 1639 - 1655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Horiguchi, H. Yurimoto, T.-K. Goh, T. Nakagawa, N. Kato, and Y. Sakai Peroxisomal Catalase in the Methylotrophic Yeast Candida boidinii: Transport Efficiency and Metabolic Significance J. Bacteriol., November 1, 2001; 183(21): 6372 - 6383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. J. Klein, P. Barnett, G. Bottger, D. Konings, H. F. Tabak, and B. Distel Recognition of Peroxisomal Targeting Signal Type 1 by the Import Receptor Pex5p J. Biol. Chem., April 27, 2001; 276(18): 15034 - 15041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Reguenga, M. E. M. Oliveira, A. M. M. Gouveia, C. Sa-Miranda, and J. E. Azevedo Characterization of the Mammalian Peroxisomal Import Machinery. Pex2p, Pex5p, Pex12p, AND Pex14p ARE SUBUNITS OF THE SAME PROTEIN ASSEMBLY J. Biol. Chem., August 3, 2001; 276(32): 29935 - 29942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Saidowsky, G. Dodt, K. Kirchberg, A. Wegner, W. Nastainczyk, W.-H. Kunau, and W. Schliebs The Di-aromatic Pentapeptide Repeats of the Human Peroxisome Import Receptor PEX5 Are Separate High Affinity Binding Sites for the Peroxisomal Membrane Protein PEX14 J. Biol. Chem., September 7, 2001; 276(37): 34524 - 34529. [Abstract] [Full Text] [PDF]
|
|
