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Vol. 11, Issue 11, 4005-4018, November 2000

Fission Yeast Int6 Is Not Essential for Global Translation Initiation, but Deletion of int6⁺ Causes Hypersensitivity to Caffeine and Affects Spore Formation

Amitabha Bandyopadhyay,^* Tomohiro Matsumoto, and Umadas Maitra^*

 ^*Department of Developmental and Molecular Biology and  Departments of Radiation Oncology and Cell Biology^, Albert Einstein College of Medicine of Yeshiva University, Jack and Pearl Resnick Campus, Bronx, New York 10461

Mammalian INT6 protein has been considered to be a subunit of the eukaryotic translation initiation factor, eIF3. The Int6 locus is also known as a common integration site of mouse mammary tumor virus (MMTV). However, the function of Int6 in translation initiation and the mechanism of Int6-mediated tumor induction are yet to be explored. In this study, the fission yeast, Schizosaccharomyces pombe, int6⁺, which is 43% identical to the mammalian counterpart, was deleted. Despite the evidence that the majority of Int6 protein was associated with 40S particles in this organism, strains lacking int6⁺ (int6) were viable and showed only moderate inhibition in the rate of in vivo global protein synthesis. Polysome profile analysis showed no apparent defects in translation initiation. int6 exhibited a hypersensitivity to caffeine, which could be suppressed by the addition of sorbitol to the growth medium. This and other phenotypes would imply that int6⁺ is required for the integrity of cell membrane. In meiosis, int6 produced incomplete tetrads frequently. High dosage expression of a truncated mutant of int6⁺ conferred a hypersensitivity to caffeine, but did not cause the defect in meiosis. A possible link between the function of int6⁺ and the int6-phenotypes is discussed.

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Corresponding authors. E-mail addresses: U. Maitra; maitra{at}aecom.yu.edu and T. Matsumoto; tmatsumo{at}aecom.yu.edu.

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Molecular Biology of the Cell
Vol. 11, 4005-4018, November 2000
Copyright © 2000 by The American Society for Cell Biology

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