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Vol. 11, Issue 12, 4309-4321, December 2000
and
*Department of Biochemistry, Howard Hughes Medical Institute,
Stanford University School of Medicine, Stanford, California
94305-5307; and The PHO regulatory pathway is involved in the acquisition of
phosphate (Pi) in the yeast Saccharomyces
cerevisiae. When extracellular Pi concentrations
are low, several genes are transcriptionally induced by this pathway,
which includes the Pho4 transcriptional activator, the Pho80-Pho85
cyclin-CDK pair, and the Pho81 CDK inhibitor. In an attempt to identify
all the components regulated by this system, a whole-genome DNA
microarray analysis was employed, and 22 PHO-regulated genes were
identified. The promoter regions of 21 of these genes contained at
least one copy of a sequence that matched the Pho4 recognition site.
Eight of these genes, PHM1-PHM8, had no previously
defined function in phosphate metabolism. The amino acid sequences of
PHM1 (YFL004w), PHM2 (YPL019c),
PHM3 (YJL012c), and PHM4 (YER072w) are
32-56% identical. The phm3 and phm4
single mutants and the phm1 phm2 double
mutant were each severely deficient in accumulation of inorganic
polyphosphate (polyP) and Pi. The phenotype of the
phm5 mutant suggests that PHM5 (YDR452w)
is essential for normal catabolism of polyP in the yeast vacuole. Taken
together, the results reveal important new features of a genetic system
that plays a critical role in Pi acquisition and polyP
metabolism in yeast.
Department of Biochemistry and
Biophysics, University of California at San Francisco, 513 Parnassus
Ave., San Francisco, California 94143
Corresponding author: E-mail address:
pbrown{at}cmgm.stanford.edu.
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