![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 12, 4323-4337, December 2000
and§
*Biodesign Research Group, Institute of Physical and Chemical
Research (RIKEN), Wako, Saitama, 351-0198, Japan;
The nuclear lamina is an important determinant of nuclear
architecture. Mutations in A-type but not B-type lamins cause a range
of human genetic disorders, including muscular dystrophy. Dominant
mutations in nuclear lamin proteins have been shown to disrupt a
preformed lamina structure in Xenopus egg extracts. Here, a series of deletion mutations in lamins A and B1 were evaluated for their ability to disrupt lamina structure in Chinese hamster ovary cells. Deletions of either the lamin A "head" domain
or the C-terminal CaaX domain formed intranuclear aggregates and resulted in the disruption of endogenous lamins A/C but not lamins B1/B2. By contrast, "head-less" lamin B1 localized to the nuclear rim with no detectable effect on endogenous lamins, whereas lamin B1
CaaX domain deletions formed intranuclear aggregates, disrupting endogenous lamins A/C but not lamins B1/B2. Filter binding assays revealed that a head/CaaX domain lamin B1 mutant interacted much more
strongly with lamins A/C than with lamins B1/B2. Regulated induction of
this mutant in stable cell lines resulted in the rapid elimination of
all detectable lamin A protein, whereas lamin C was trapped in a
soluble form within the intranuclear aggregates. In contrast to results
in Xenopus egg extracts, dominant negative lamin B1 (but
not lamin A) mutants trapped replication proteins involved in both the
initiation and elongation phases of replication but did not effect
cellular growth rates or the assembly of active replication centers. We
conclude that elimination of the CaaX domain in lamin B1 and
elimination of either the CaaX or head domain in lamin A constitute
dominant mutations that can disrupt A-type but not B-type lamins,
highlighting important differences in the way that A- and B-type lamins
are integrated into the lamina.
Department of Biological Sciences, University of Durham,
Durham City, DH1 3LE, United Kingdom; and Department of
Biochemistry and Molecular Biology, State University of New York
Upstate Medical University, Syracuse, New York 13210
This article has been cited by other articles:
|
|
 |
|
  C.-P. Lee, Y.-H. Huang, S.-F. Lin, Y. Chang, Y.-H. Chang, K. Takada, and M.-R. Chen Epstein-Barr Virus BGLF4 Kinase Induces Disassembly of the Nuclear Lamina To Facilitate Virion Production J. Virol., December 1, 2008; 82(23): 11913 - 11926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Gehrig, R. B. Cornell, and N. D. Ridgway Expansion of the Nucleoplasmic Reticulum Requires the Coordinated Activity of Lamins and CTP:Phosphocholine Cytidylyltransferase {alpha} Mol. Biol. Cell, January 1, 2008; 19(1): 237 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. van Tintelen, R. A. Tio, W. S. Kerstjens-Frederikse, J. H. van Berlo, L. G. Boven, A. J.H. Suurmeijer, S. J. White, J. T. den Dunnen, G. J. te Meerman, Y. J. Vos, et al. Severe Myocardial Fibrosis Caused by a Deletion of the 5' End of the Lamin A/C Gene J. Am. Coll. Cardiol., June 26, 2007; 49(25): 2430 - 2439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mou, T. Forest, and J. D. Baines US3 of Herpes Simplex Virus Type 1 Encodes a Promiscuous Protein Kinase That Phosphorylates and Alters Localization of Lamin A/C in Infected Cells J. Virol., June 15, 2007; 81(12): 6459 - 6470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Young, M. Meta, S. H. Yang, and L. G. Fong Prelamin A Farnesylation and Progeroid Syndromes J. Biol. Chem., December 29, 2006; 281(52): 39741 - 39745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. V. Broers, F. C. S. Ramaekers, G. Bonne, R. B. Yaou, and C. J. Hutchison Nuclear lamins: laminopathies and their role in premature ageing. Physiol Rev, July 1, 2006; 86(3): 967 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Delbarre, M. Tramier, M. Coppey-Moisan, C. Gaillard, J.-C. Courvalin, and B. Buendia The truncated prelamin A in Hutchinson-Gilford progeria syndrome alters segregation of A-type and B-type lamin homopolymers Hum. Mol. Genet., April 1, 2006; 15(7): 1113 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Zastrow, D. B. Flaherty, G. M. Benian, and K. L. Wilson Nuclear Titin interacts with A- and B-type lamins in vitro and in vivo J. Cell Sci., January 15, 2006; 119(2): 239 - 249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Brachner, S. Reipert, R. Foisner, and J. Gotzmann LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins J. Cell Sci., December 15, 2005; 118(24): 5797 - 5810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G. Young, L. G. Fong, and S. Michaelis Thematic Review Series: Lipid Posttranslational Modifications. Prelamin A, Zmpste24, misshapen cell nuclei, and progeria--new evidence suggesting that protein farnesylation could be important for disease pathogenesis J. Lipid Res., December 1, 2005; 46(12): 2531 - 2558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Simpson-Holley, R. C. Colgrove, G. Nalepa, J. W. Harper, and D. M. Knipe Identification and Functional Evaluation of Cellular and Viral Factors Involved in the Alteration of Nuclear Architecture during Herpes Simplex Virus 1 Infection J. Virol., October 15, 2005; 79(20): 12840 - 12851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. I. Toth, S. H. Yang, X. Qiao, A. P. Beigneux, M. H. Gelb, C. L. Moulson, J. H. Miner, S. G. Young, and L. G. Fong Blocking protein farnesyltransferase improves nuclear shape in fibroblasts from humans with progeroid syndromes PNAS, September 6, 2005; 102(36): 12873 - 12878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Yang, M. O. Bergo, J. I. Toth, X. Qiao, Y. Hu, S. Sandoval, M. Meta, P. Bendale, M. H. Gelb, S. G. Young, et al. Blocking protein farnesyltransferase improves nuclear blebbing in mouse fibroblasts with a targeted Hutchinson-Gilford progeria syndrome mutation PNAS, July 19, 2005; 102(29): 10291 - 10296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Mariappan and V. K. Parnaik Sequestration of pRb by Cyclin D3 Causes Intranuclear Reorganization of Lamin A/C during Muscle Cell Differentiation Mol. Biol. Cell, April 1, 2005; 16(4): 1948 - 1960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Prufert, A. Vogel, and G. Krohne The lamin CxxM motif promotes nuclear membrane growth J. Cell Sci., December 1, 2004; 117(25): 6105 - 6116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ralle, C. Grund, W. W. Franke, and R. Stick Intranuclear membrane structure formations by CaaX-containing nuclear proteins J. Cell Sci., December 1, 2004; 117(25): 6095 - 6104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Izumi, F. Yatagai, and F. Hanaoka Localization of Human Mcm10 Is Spatially and Temporally Regulated during the S Phase J. Biol. Chem., July 30, 2004; 279(31): 32569 - 32577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Vergnes, M. Peterfy, M. O. Bergo, S. G. Young, and K. Reue Lamin B1 is required for mouse development and nuclear integrity PNAS, July 13, 2004; 101(28): 10428 - 10433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Reynolds, L. Liang, and J. D. Baines Conformational Changes in the Nuclear Lamina Induced by Herpes Simplex Virus Type 1 Require Genes UL31 and UL34 J. Virol., June 1, 2004; 78(11): 5564 - 5575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Zastrow, S. Vlcek, and K. L. Wilson Proteins that bind A-type lamins: integrating isolated clues J. Cell Sci., March 1, 2004; 117(7): 979 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. I. Kumaran, B. Muralikrishna, and V. K. Parnaik Lamin A/C speckles mediate spatial organization of splicing factor compartments and RNA polymerase II transcription J. Cell Biol., December 9, 2002; 159(5): 783 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Markiewicz, T. Dechat, R. Foisner, Roy. A Quinlan, and C. J. Hutchison Lamin A/C Binding Protein LAP2alpha Is Required for Nuclear Anchorage of Retinoblastoma Protein Mol. Biol. Cell, December 1, 2002; 13(12): 4401 - 4413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Arbustini, A. Pilotto, A. Repetto, M. Grasso, A. Negri, M. Diegoli, C. Campana, L. Scelsi, E. Baldini, A. Gavazzi, et al. Autosomal dominant dilated cardiomyopathy with atrioventricular block: a lamin A/C defect-related disease J. Am. Coll. Cardiol., March 20, 2002; 39(6): 981 - 990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ostlund, G. Bonne, K. Schwartz, and H. J. Worman Properties of lamin A mutants found in Emery-Dreifuss muscular dystrophy, cardiomyopathy and Dunnigan-type partial lipodystrophy J. Cell Sci., March 14, 2002; 114(24): 4435 - 4445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. H. Raharjo, P. Enarson, T. Sullivan, C. L. Stewart, and B. Burke Nuclear envelope defects associated with LMNA mutations cause dilated cardiomyopathy and Emery-Dreifuss muscular dystrophy J. Cell Sci., March 14, 2002; 114(24): 4447 - 4457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Izumi, F. Yatagai, and F. Hanaoka Cell Cycle-dependent Proteolysis and Phosphorylation of Human Mcm10 J. Biol. Chem., December 14, 2001; 276(51): 48526 - 48531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Schirmer, T. Guan, and L. Gerace Involvement of the Lamin Rod Domain in Heterotypic Lamin Interactions Important for Nuclear Organization J. Cell Biol., April 24, 2001; 153(3): 479 - 490. [Abstract] [Full Text] [PDF]
|
|
