![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 12, 4369-4380, December 2000
1 Relocation: Role in
Resumption of Meiosis in the Mouse Oocyte
Institut National de la Santé et de la Recherche
Médicale Unité 355 and Institut Fédératif de
Recherche sur les Cytokines, 92140 Clamart, France
The location of the phospholipase C 
1-isoform (PLC-1) in the
mouse oocyte and its role in the resumption of meiosis were examined.
We used specific monoclonal antibodies to monitor the in vitro dynamics
of the subcellular distribution of the enzyme from the release of the
oocyte from the follicle until breakdown of the germinal vesicle (GVBD)
by Western blotting, electron microscope immunohistochemistry, and
confocal microscope immunofluorescence. PLC-1 became relocated to
the oocyte cortex and the nucleoplasm during the G2/M transition,
mainly in the hour preceding GVBD. The enzyme was a 150-kDa protein,
corresponding to PLC-1a. Its synthesis in the cytoplasm increased
during this period, and it accumulated in the nucleoplasm. GVBD was
dramatically inhibited by the microinjection of anti-PLC-1
monoclonal antibody into the germinal vesicle (GV) only when
this accumulation was at its maximum. In contrast, PLC-
1 was absent
from the GV from the time of release from the follicle until 1 h
later, and microinjection of anti-PLC-1 into the GV did not affect
GVBD. Our results demonstrate a relationship between the relocation of
PLC-1 and its role in the first step of meiosis.
This article has been cited by other articles:
|
|
 |
|
  A. Pesty, O. Broca, C. Poirot, and B. Lefevre The Role of PLC{beta}1 in the Control of Oocyte Meiosis During Folliculogenesis Reproductive Sciences, September 1, 2008; 15(7): 661 - 672. [Abstract] [PDF]
|
|
|
|
 |
|
 K. Kuroda, M. Ito, T. Shikano, T. Awaji, A. Yoda, H. Takeuchi, K. Kinoshita, and S. Miyazaki The Role of X/Y Linker Region and N-terminal EF-hand Domain in Nuclear Translocation and Ca2+ Oscillation-inducing Activities of Phospholipase C{zeta}, a Mammalian Egg-activating Factor J. Biol. Chem., September 22, 2006; 281(38): 27794 - 27805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Avazeri, A.-M. Courtot, and B. Lefevre Regulation of spontaneous meiosis resumption in mouse oocytes by various conventional PKC isozymes depends on cellular compartmentalization J. Cell Sci., October 1, 2004; 117(21): 4969 - 4978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Marangos, G. FitzHarris, and J. Carroll Ca2+ oscillations at fertilization in mammals are regulated by the formation of pronuclei Development, April 1, 2003; 130(7): 1461 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. T. Y. Chiu, M. S. Rogers, C. Briton-Jones, and C. Haines Effects of myo-inositol on the in-vitro maturation and subsequent development of mouse oocytes Hum. Reprod., February 1, 2003; 18(2): 408 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Irvine Nuclear Lipid Signaling Sci. Signal., September 17, 2002; 2002(150): re13 - re13. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Neri, R. Bortul, P. Borgatti, G. Tabellini, G. Baldini, S. Capitani, and A. M. Martelli Proliferating or Differentiating Stimuli Act on Different Lipid-dependent Signaling Pathways in Nuclei of Human Leukemia Cells Mol. Biol. Cell, March 1, 2002; 13(3): 947 - 964. [Abstract] [Full Text] [PDF]
|
|
