![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 2, 481-495, February 2000
and
*Structural Cell Biology Unit, Department of Medical Anatomy, The
Panum Institute, University of Copenhagen, DK-2200 Copenhagen, Denmark;
It is well established that dynamin is involved in
clathrin-dependent endocytosis, but relatively little is known about
possible intracellular functions of this GTPase. Using confocal
imaging, we found that endogenous dynamin was associated with the
plasma membrane, the trans-Golgi network, and a
perinuclear cluster of cation-independent mannose 6-phosphate receptor
(CI-MPR)-containing structures. By electron microscopy (EM), it was
shown that these structures were late endosomes and that the endogenous
dynamin was preferentially localized to tubulo-vesicular appendices on these late endosomes. Upon induction of the dominant-negative dynK44A
mutant, confocal microscopy demonstrated a redistribution of the CI-MPR
in mutant-expressing cells. Quantitative EM analysis of the ratio of
CI-MPR to lysosome-associated membrane protein-1 in endosome profiles
revealed a higher colocalization of the two markers in
dynK44A-expressing cells than in control cells. Western blot analysis
showed that dynK44A-expressing cells had an increased cellular
procathepsin D content. Finally, EM revealed that in dynK44A-expressing
cells, endosomal tubules containing CI-MPR were formed. These results
are in contrast to recent reports that dynamin-2 is exclusively
associated with endocytic structures at the plasma membrane. They
suggest instead that endogenous dynamin also plays an important role in
the molecular machinery behind the recycling of the CI-MPR from
endosomes to the trans-Golgi network, and we propose
that dynamin is required for the final scission of vesicles budding
from endosome tubules.
Institute for Cancer Research, The Norwegian Radium
Hospital, Montebello, 0310 Oslo, Norway; and §Unite de
Biologie Cellulaire, Christian de Duve Institute of Cellular
Pathology, B-1200 Bruxelles, Belgium
Present address: Consorzio Mario Negri Sud,
66030 S. Maria Imbaro, Italy.
Corresponding author. E-mail address:
b.v.deurs{at}mai.ku.dk.
This article has been cited by other articles:
|
|
 |
|
  C. A. Konopka, S. K. Backues, and S. Y. Bednarek Dynamics of Arabidopsis Dynamin-Related Protein 1C and a Clathrin Light Chain at the Plasma Membrane PLANT CELL, May 1, 2008; 20(5): 1363 - 1380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Tribl, M. Gerlach, K. Marcus, E. Asan, T. Tatschner, T. Arzberger, H. E. Meyer, G. Bringmann, and P. Riederer "Subcellular Proteomics" of Neuromelanin Granules Isolated from the Human Brain Mol. Cell. Proteomics, July 1, 2005; 4(7): 945 - 957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Pitts, M. A. McNiven, and Y. Yoon Mitochondria-specific Function of the Dynamin Family Protein DLP1 Is Mediated by Its C-terminal Domains J. Biol. Chem., November 26, 2004; 279(48): 50286 - 50294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. U. Lauvrak, M. L. Torgersen, and K. Sandvig Efficient endosome-to-Golgi transport of Shiga toxin is dependent on dynamin and clathrin J. Cell Sci., May 1, 2004; 117(11): 2321 - 2331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sanchez-San Martin, T. Lopez, C. F. Arias, and S. Lopez Characterization of Rotavirus Cell Entry J. Virol., March 1, 2004; 78(5): 2310 - 2318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Fink The Hereditary Spastic Paraplegias: Nine Genes and Counting Arch Neurol, August 1, 2003; 60(8): 1045 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Di, D. J. Nelson, V. Bindokas, M. E. Brown, F. Libunao, and H. C. Palfrey Dynamin Regulates Focal Exocytosis in Phagocytosing Macrophages Mol. Biol. Cell, May 1, 2003; 14(5): 2016 - 2028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-G. Iversen, G. Skretting, B. van Deurs, and K. Sandvig From the Cover: Clathrin-coated pits with long, dynamin-wrapped necks upon expression of a clathrin antisense RNA PNAS, April 29, 2003; 100(9): 5175 - 5180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Deloche and R. W. Schekman Vps10p Cycles between the TGN and the Late Endosome via the Plasma Membrane in Clathrin Mutants Mol. Biol. Cell, December 1, 2002; 13(12): 4296 - 4307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Sieczkarski and G. R. Whittaker Influenza Virus Can Enter and Infect Cells in the Absence of Clathrin-Mediated Endocytosis J. Virol., September 11, 2002; 76(20): 10455 - 10464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-L. Eskelinen, A. L. Illert, Y. Tanaka, G. Schwarzmann, J. Blanz, K. von Figura, and P. Saftig Role of LAMP-2 in Lysosome Biogenesis and Autophagy Mol. Biol. Cell, September 1, 2002; 13(9): 3355 - 3368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Szaszak, Z. Gaborik, G. Turu, P. S. McPherson, A. J. L. Clark, K. J. Catt, and L. Hunyady Role of the Proline-rich Domain of Dynamin-2 and Its Interactions with Src Homology 3 Domains during Endocytosis of the AT1 Angiotensin Receptor J. Biol. Chem., June 7, 2002; 277(24): 21650 - 21656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Sieczkarski and G. R. Whittaker Dissecting virus entry via endocytosis J. Gen. Virol., June 1, 2002; 83(7): 1535 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. U. Lauvrak, A. Llorente, T.-G. Iversen, and K. Sandvig Selective regulation of the Rab9-independent transport of ricin to the Golgi apparatus by calcium J. Cell Sci., January 9, 2002; 115(17): 3449 - 3456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yoon, K. R. Pitts, and M. A. McNiven Mammalian Dynamin-like Protein DLP1 Tubulates Membranes Mol. Biol. Cell, September 1, 2001; 12(9): 2894 - 2905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-G. Iversen, G. Skretting, A. Llorente, P. Nicoziani, B. van Deurs, and K. Sandvig Endosome to Golgi Transport of Ricin Is Independent of Clathrin and of the Rab9- and Rab11-GTPases Mol. Biol. Cell, July 1, 2001; 12(7): 2099 - 2107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Llorente, K Prydz, M Sprangers, G Skretting, S. Kolset, and K Sandvig Proteoglycan synthesis is increased in cells with impaired clathrin-dependent endocytosis J. Cell Sci., January 1, 2001; 114(2): 335 - 343. [Abstract] [PDF]
|
|
|
|
 |
|
 S. F. Nothwehr, S.-A. Ha, and P. Bruinsma Sorting of Yeast Membrane Proteins into an Endosome-to-Golgi Pathway Involves Direct Interaction of Their Cytosolic Domains with Vps35p J. Cell Biol., October 18, 2000; 151(2): 297 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Bayer, D. Schober, M. Huttinger, D. Blaas, and R. Fuchs Inhibition of Clathrin-dependent Endocytosis Has Multiple Effects on Human Rhinovirus Serotype 2 Cell Entry J. Biol. Chem., February 2, 2001; 276(6): 3952 - 3962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Harsay and R. Schekman A subset of yeast vacuolar protein sorting mutants is blocked in one branch of the exocytic pathway J. Cell Biol., January 21, 2002; 156(2): 271 - 286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. van Dam and W. Stoorvogel Dynamin-dependent Transferrin Receptor Recycling by Endosome-derived Clathrin-coated Vesicles Mol. Biol. Cell, January 1, 2002; 13(1): 169 - 182. [Abstract] [Full Text] [PDF]
|
|
