![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 2, 497-510, February 2000
ák,
t
pánka
Cermanová,
ina
Jirsová,
ka*
Department of Cell Biology, Institute of Experimental Medicine,
Academy of Sciences of Czech Republic, and Laboratory of Gene
Expression, Third Medical Faculty, Charles University, 128 00 Prague,
Czech Republic
In the present study, the spatial organization of
intron-containing pre-mRNAs of Epstein-Barr virus (EBV) genes relative
to location of splicing factors is investigated. The intranuclear position of transcriptionally active EBV genes, as well as of nascent
transcripts, is found to be random with respect to the speckled
accumulations of splicing factors (SC35 domains) in Namalwa cells,
arguing against the concept of the locus-specific organization of mRNA
genes with respect to the speckles. Microclusters of splicing factors
are, however, frequently superimposed on nascent transcript sites. The
transcript environment is a dynamic structure consisting of both
nascent and released transcripts, i.e., the track-like transcript
environment. Both EBV sequences of the chromosome 1 homologue are
usually associated with the track, are transcriptionally active, and
exhibit in most cases a polar orientation. In contrast to nascent
transcripts (in the form of spots), the association of a
post-transcriptional pool of viral pre-mRNA (in the form of tracks)
with speckles is not random and is further enhanced in
transcriptionally silent cells when splicing factors are sequestered in
enlarged accumulations. The transcript environment reflects the
intranuclear transport of RNA from the sites of transcription to SC35
domains, as shown by concomitant mapping of DNA, RNA, and splicing
factors. No clear vectorial intranuclear trafficking of transcripts
from the site of synthesis toward the nuclear envelope for export into
the cytoplasm is observed. Using Namalwa and Raji cell lines, a
correlation between the level of viral gene transcription and splicing
factor accumulation within the viral transcript environment has been
observed. This supports a concept that the level of transcription can
alter the spatial relationship among intron-containing genes, their
transcripts, and speckles attributable to various levels of splicing
factors recruited from splicing factor reservoirs. Electron microscopic
in situ hybridization studies reveal that the released transcripts are
directed toward reservoirs of splicing factors organized in clusters of
interchromatin granules. Our results point to the bidirectional
intranuclear movement of macromolecular complexes between
intron-containing genes and splicing factor reservoirs: the recruitment
of splicing factors to transcription sites and movement of released
transcripts from DNA loci to reservoirs of splicing factors.
This article has been cited by other articles:
|
|
 |
|
  J. M. Pawlicki and J. A. Steitz Primary microRNA transcript retention at sites of transcription leads to enhanced microRNA production J. Cell Biol., July 14, 2008; 182(1): 61 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Holt, S. Mittal, D. Furling, G. S Butler-Browne, J. D. Brook, and G. E. Morris Defective mRNA in myotonic dystrophy accumulates at the periphery of nuclear splicing speckles Genes Cells, September 1, 2007; 12(9): 1035 - 1048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Royo, E. Basyuk, V. Marty, M. Marques, E. Bertrand, and J. Cavaille Bsr, a Nuclear-retained RNA with Monoallelic Expression Mol. Biol. Cell, August 1, 2007; 18(8): 2817 - 2827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. G. Cline and W. J. Nelson Characterization of Mammalian Par 6 as a Dual-Location Protein Mol. Cell. Biol., June 15, 2007; 27(12): 4431 - 4443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Grunwald, B. Spottke, V. Buschmann, and U. Kubitscheck Intranuclear Binding Kinetics and Mobility of Single Native U1 snRNP Particles in Living Cells Mol. Biol. Cell, December 1, 2006; 17(12): 5017 - 5027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Tillemans, I. Leponce, G. Rausin, L. Dispa, and P. Motte Insights into Nuclear Organization in Plants as Revealed by the Dynamic Distribution of Arabidopsis SR Splicing Factors PLANT CELL, November 1, 2006; 18(11): 3218 - 3234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Mangs, H. J.L. Speirs, C. Goy, D. J. Adams, M. A. Markus, and B. J. Morris XE7: A novel splicing factor that interacts with ASF/SF2 and ZNF265 Nucleic Acids Res., October 18, 2006; 34(17): 4976 - 4986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Binnie, P. Castelo-Branco, J. Monks, and N. J. Proudfoot Homologous gene sequences mediate transcription-domain formation J. Cell Sci., September 15, 2006; 119(18): 3876 - 3887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sastri, D. M. Barraclough, P. T. Carmichael, and S. S. Taylor A-kinase-interacting protein localizes protein kinase A in the nucleus PNAS, January 11, 2005; 102(2): 349 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Vecerova, K. Koberna, J. Malinsky, E. Soutoglou, T. Sullivan, C. L. Stewart, I. Raska, and T. Misteli Formation of Nuclear Splicing Factor Compartments Is Independent of Lamins A/C Mol. Biol. Cell, November 1, 2004; 15(11): 4904 - 4910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Molenaar, A. Abdulle, A. Gena, H. J. Tanke, and R. W. Dirks Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells J. Cell Biol., April 26, 2004; 165(2): 191 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Shopland, C. V. Johnson, M. Byron, J. McNeil, and J. B. Lawrence Clustering of multiple specific genes and gene-rich R-bands around SC-35 domains: evidence for local euchromatic neighborhoods J. Cell Biol., September 15, 2003; 162(6): 981 - 990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bartl, J. Ban, H. Weninger, G. Jug, and H. Kovar A small nuclear RNA, hdm365, is the major processing product of the human mdm2 gene Nucleic Acids Res., February 15, 2003; 31(4): 1136 - 1147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Donev, R. Horton, S. Beck, T. Doneva, R. Vatcheva, W. R. Bowen, and D. Sheer Recruitment of Heterogeneous Nuclear Ribonucleoprotein A1 in Vivo to the LMP/TAP Region of the Major Histocompatibility Complex J. Biol. Chem., February 7, 2003; 278(7): 5214 - 5226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Neugebauer On the importance of being co-transcriptional J. Cell Sci., October 15, 2002; 115(20): 3865 - 3871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Snaar, P. Verdijk, H. J. Tanke, and R. W. Dirks Kinetics of HCMV immediate early mRNA expression in stably transfected fibroblasts J. Cell Sci., January 15, 2002; 115(2): 321 - 328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Hattinger, A. G. Jochemsen, H. J. Tanke, and R. W. Dirks Induction of p21 mRNA Synthesis After Short-wavelength UV Light Visualized in Individual Cells by RNA FISH J. Histochem. Cytochem., January 1, 2002; 50(1): 81 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bell, L. J. Montaner, and G. G. Maul Accumulation and Intranuclear Distribution of Unintegrated Human Immunodeficiency Virus Type 1 DNA J. Virol., August 15, 2001; 75(16): 7683 - 7691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shav-Tal, M. Cohen, S. Lapter, B. Dye, J. G. Patton, J. Vandekerckhove, and D. Zipori Nuclear Relocalization of the Pre-mRNA Splicing Factor PSF during Apoptosis Involves Hyperphosphorylation, Masking of Antigenic Epitopes, and Changes in Protein Interactions Mol. Biol. Cell, August 1, 2001; 12(8): 2328 - 2340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. Kim, S. Noguchi, Y. K. Hayashi, T. Tsukahara, T. Shimizu, and K. Arahata The product of an oculopharyngeal muscular dystrophy gene, poly(A)-binding protein 2, interacts with SKIP and stimulates muscle-specific gene expression Hum. Mol. Genet., May 1, 2001; 10(11): 1129 - 1139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Prespliceosomal Assembly on Microinjected Precursor mRNA Takes Place in Nuclear Speckles Mol. Biol. Cell, February 1, 2001; 12(2): 393 - 406. [Abstract] [Full Text]
|
|
|
|
|
|
C. Johnson, D. Primorac, M. McKinstry, J. McNeil, D. Rowe, and J. B. Lawrence Tracking COL1A1 RNA in Osteogenesis Imperfecta: Splice-defective Transcripts Initiate Transport from the Gene but Are Retained within the SC35 Domain J. Cell Biol., August 7, 2000; 150(3): 417 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Shopland and J. B. Lawrence Seeking Common Ground in Nuclear Complexity J. Cell Biol., July 11, 2000; 150(1): F1 - F4. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T Misteli Cell biology of transcription and pre-mRNA splicing: nuclear architecture meets nuclear function J. Cell Sci., January 6, 2000; 113(11): 1841 - 1849. [Abstract] [PDF]
|
|
