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Vol. 11, Issue 2, 593-611, February 2000
and
*Department of Biology, Swarthmore College, Swarthmore,
Pennsylvania 19081; and Cytokinesis in Saccharomyces cerevisiae occurs by
the concerted action of the actomyosin system and septum formation.
Here we report on the roles of HOF1,
BNI1, and BNR1 in cytokinesis, focusing
on Hof1p. Deletion of HOF1 causes a
temperature-sensitive defect in septum formation. A Hof1p ring forms on
the mother side of the bud neck in G2/M, followed by the formation of a
daughter-side ring. Around telophase, Hof1p is phosphorylated and the
double rings merge into a single ring that contracts slightly and may colocalize with the actomyosin structure. Upon septum formation, Hof1p
splits into two rings, disappearing upon cell separation. Hof1p
localization is dependent on septins but not Myo1p. Synthetic lethality
suggests that Bni1p and Myo1p belong to one functional pathway, whereas
Hof1p and Bnr1p belong to another. These results suggest that Hof1p may
function as an adapter linking the primary septum synthesis machinery
to the actomyosin system. The formation of the actomyosin ring is not
affected by bni1Department of Cell and
Developmental Biology, University of Pennsylvania School of Medicine,
Philadelphia, Pennsylvania 19104-6058
, hof1, or
bnr1. However, Myo1p contraction is affected by
bni1 but not by hof1 or
bnr1. In bni1 cells that lack the
actomyosin contraction, septum formation is often slow and asymmetric,
suggesting that actomyosin contraction may provide directionality for
efficient septum formation.
Corresponding author. E-mail
address: ebi{at}mail.med.upenn.edu.
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