![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 2, 647-661, February 2000
State University of New York Upstate Medical University, Department
of Biochemistry and Molecular Biology, Syracuse, New York 13210
Aip3p/Bud6p is a regulator of cell and cytoskeletal polarity in
Saccharomyces cerevisiae that was previously identified
as an actin-interacting protein. Actin-interacting protein 3 (Aip3p) localizes at the cell cortex where cytoskeleton assembly must be
achieved to execute polarized cell growth, and deletion of AIP3 causes gross defects in cell and cytoskeletal
polarity. We have discovered that Aip3p localization is mediated by the
secretory pathway. Mutations in early- or late-acting components of the secretory apparatus lead to Aip3p mislocalization. Biochemical data
show that a pool of Aip3p is associated with post-Golgi secretory vesicles. An investigation of the sequences within Aip3p necessary for
Aip3p localization has identified a sequence within the N terminus of
Aip3p that is sufficient for directing Aip3p localization. Replacement
of the N terminus of Aip3p with a homologous region from a
Schizosaccharomyces pombe protein allows for normal
Aip3p localization, indicating that the secretory pathway-mediated
Aip3p localization pathway is conserved. Delivery of Aip3p also
requires the type V myosin motor Myo2p and its regulatory light-chain
calmodulin. These data suggest that one function of calmodulin is to
activate Myo2p's activity in the secretory pathway; this function is
likely the polarized movement of late secretory vesicles and associated Aip3p on actin cables.
This article has been cited by other articles:
|
|
 |
|
  M. Kohli, V. Galati, K. Boudier, R. W. Roberson, and P. Philippsen Growth-speed-correlated localization of exocyst and polarisome components in growth zones of Ashbya gossypii hyphal tips J. Cell Sci., December 1, 2008; 121(23): 3878 - 3889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Delgehyr, C. S. J. Lopes, C. A. Moir, S. M. Huisman, and M. Segal Dissecting the involvement of formins in Bud6p-mediated cortical capture of microtubules in S. cerevisiae J. Cell Sci., November 15, 2008; 121(22): 3803 - 3814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Haarer, A. H. Helfant, S. A. Nelson, J. A. Cooper, and D. C. Amberg Stable Preanaphase Spindle Positioning Requires Bud6p and an Apparent Interaction between the Spindle Pole Bodies and the Neck Eukaryot. Cell, May 1, 2007; 6(5): 797 - 807. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Buttery, S. Yoshida, and D. Pellman Yeast Formins Bni1 and Bnr1 Utilize Different Modes of Cortical Interaction during the Assembly of Actin Cables Mol. Biol. Cell, May 1, 2007; 18(5): 1826 - 1838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Virag and S. D. Harris Functional Characterization of Aspergillus nidulans Homologues of Saccharomyces cerevisiae Spa2 and Bud6 Eukaryot. Cell, June 1, 2006; 5(6): 881 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Shih, S. L. Reck-Peterson, R. Newitt, M. S. Mooseker, R. Aebersold, and I. Herskowitz Cell Polarity Protein Spa2P Associates With Proteins Involved In Actin Function In Saccharomyces Cerevisiae Mol. Biol. Cell, October 1, 2005; 16(10): 4595 - 4608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Moseley and B. L. Goode Differential Activities and Regulation of Saccharomyces cerevisiae Formin Proteins Bni1 and Bnr1 by Bud6 J. Biol. Chem., July 29, 2005; 280(30): 28023 - 28033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, P. Wang, A. Gangar, J. Zhang, P. Brennwald, D. TerBush, and W. Guo Lethal giant larvae proteins interact with the exocyst complex and are involved in polarized exocytosis J. Cell Biol., July 18, 2005; 170(2): 273 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Luedeke, S. B. Frei, I. Sbalzarini, H. Schwarz, A. Spang, and Y. Barral Septin-dependent compartmentalization of the endoplasmic reticulum during yeast polarized growth J. Cell Biol., June 20, 2005; 169(6): 897 - 908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Castagnetti, R. Behrens, and P. Nurse End4/Sla2 is involved in establishment of a new growth zone in Schizosaccharomyces pombe J. Cell Sci., May 1, 2005; 118(9): 1843 - 1850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zajac, X. Sun, J. Zhang, and W. Guo Cyclical Regulation of the Exocyst and Cell Polarity Determinants for Polarized Cell Growth Mol. Biol. Cell, March 1, 2005; 16(3): 1500 - 1512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Huisman and M. Segal Cortical capture of microtubules and spindle polarity in budding yeast - where's the catch? J. Cell Sci., February 1, 2005; 118(3): 463 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Pruyne, L. Gao, E. Bi, and A. Bretscher Stable and Dynamic Axes of Polarity Use Distinct Formin Isoforms in Budding Yeast Mol. Biol. Cell, November 1, 2004; 15(11): 4971 - 4989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Saito, K. Fujimura-Kamada, N. Furuta, U. Kato, M. Umeda, and K. Tanaka Cdc50p, a Protein Required for Polarized Growth, Associates with the Drs2p P-Type ATPase Implicated in Phospholipid Translocation in Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 2004; 15(7): 3418 - 3432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Feierbach, F. Verde, and F. Chang Regulation of a formin complex by the microtubule plus end protein tea1p J. Cell Biol., June 7, 2004; 165(5): 697 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Mackin, T. J. Sousou, and S. E. Erdman The PXL1 Gene of Saccharomyces cerevisiae Encodes a Paxillin-like Protein Functioning in Polarized Cell Growth Mol. Biol. Cell, April 1, 2004; 15(4): 1904 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-D. Gao, S. Albert, S. E. Tcheperegine, C. G. Burd, D. Gallwitz, and E. Bi The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization J. Cell Biol., August 18, 2003; 162(4): 635 - 646. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Shimada, S. Hara-Kuge, K. Yamashita, H. Tosaka-Shimada, L. Yanchao, L. Einan, S. Atsumi, and H. Ishikawa Localization of VIP36 in the Post-Golgi Secretory Pathway Also of Rat Parotid Acinar Cells J. Histochem. Cytochem., August 1, 2003; 51(8): 1057 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yuzyuk and D. C. Amberg Actin Recovery and Bud Emergence in Osmotically Stressed Cells Requires the Conserved Actin Interacting Mitogen-activated Protein Kinase Kinase Kinase Ssk2p/MTK1 and the Scaffold Protein Spa2p Mol. Biol. Cell, July 1, 2003; 14(7): 3013 - 3026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jensen, M. Geymonat, A. L. Johnson, M. Segal, and L. H. Johnston Spatial regulation of the guanine nucleotide exchange factor Lte1 in Saccharomyces cerevisiae J. Cell Sci., March 14, 2003; 115(24): 4977 - 4991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Misu, K. Fujimura-Kamada, T. Ueda, A. Nakano, H. Katoh, and K. Tanaka Cdc50p, a Conserved Endosomal Membrane Protein, Controls Polarized Growth in Saccharomyces cerevisiae Mol. Biol. Cell, February 1, 2003; 14(2): 730 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ho and A. Bretscher Ras Regulates the Polarity of the Yeast Actin Cytoskeleton through the Stress Response Pathway Mol. Biol. Cell, June 1, 2001; 12(6): 1541 - 1555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jin and D. C. Amberg Fission Yeast Aip3p (spAip3p) Is Required for an Alternative Actin-directed Polarity Program Mol. Biol. Cell, May 1, 2001; 12(5): 1275 - 1291. [Abstract] [Full Text]
|
|
|
|
|
|
C. Padmashree and U Surana Cdc28-Clb mitotic kinase negatively regulates bud site assembly in the budding yeast J. Cell Sci., January 1, 2001; 114(1): 207 - 218. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Freedman, A. Porter, and B. Haarer Mutational and hyperexpression-induced disruption of bipolar budding in yeast Microbiology, November 1, 2000; 146(11): 2833 - 2843. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Jaquenoud and M. Peter Gic2p May Link Activated Cdc42p to Components Involved in Actin Polarization, Including Bni1p and Bud6p (Aip3p) Mol. Cell. Biol., September 1, 2000; 20(17): 6244 - 6258. [Abstract] [Full Text]
|
|
