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Vol. 11, Issue 3, 1011-1022, March 2000

Discontinuous Actin Hexagon, a Protein Essential for Cortical Furrow Formation in Drosophila, Is Membrane Associated and Hyperphosphorylated

Claire X. Zhang,* Wendy F. Rothwell,dagger William Sullivan,dagger and Tao-shih Hsieh*Dagger

 *Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710; and  dagger Sinsheimer Laboratories, Department of Biology, University of California, Santa Cruz, California 95064

discontinuous actin hexagon (dah) is a maternal-effect gene essential for the formation of cortical furrows during Drosophila embryogenesis, and DAH protein colocalizes with actin in these furrows. Biochemical fractionation experiments presented here demonstrate that DAH is highly enriched in the membrane fraction and that its membrane association is resistant to high-salt and alkaline washes. Furthermore, it partitions into the detergent phase of the Triton X-114 solution, indicating its tight binding to the membranes. DAH can also interact with the actin cytoskeleton, because a fraction of DAH remains insoluble to nonionic detergent along with actin. These biochemical characterizations suggest that DAH may play a role in the linkage of the actin cytoskeleton to membranes. Using phosphatase inhibitors, we detected multiple phosphorylated forms of DAH in embryonic extracts. The DAH phosphorylation peaks during cellularization, a stage at which DAH function is critical. A kinase activity is coimmunoprecipitated with the DAH complex and hyperphosphorylates DAH in vitro. Purified casein kinase I can also hyperphosphorylate DAH in the immune complex. Both DAH localization and phosphorylation are disrupted in another maternal-effect mutant, nuclear-fallout. It is possible that nuclear-fallout collaborates with dah and directs DAH protein localization to the cortical furrows.

Dagger Corresponding author. E-mail address: hsieh{at}biochem.duke.edu.

Molecular Biology of the Cell
Vol. 11, 1011-1022, March 2000
Copyright © 2000 by The American Society for Cell Biology


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