Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Albrecht, E. B.
Right arrow Articles by Patterson, T. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Albrecht, E. B.
Right arrow Articles by Patterson, T. E.

Vol. 11, Issue 3, 873-886, March 2000

Mechanisms of sod2 Gene Amplification in Schizosaccharomyces pombe

Elizabeth B. Albrecht, Aaron B. Hunyady, George R. Stark,* and Thomas E. Patterson

Department of Molecular Biology, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195

Gene amplification in eukaryotes plays an important role in drug resistance, tumorigenesis, and evolution. The Schizosaccharomyces pombe sod2 gene provides a useful model system to analyze this process. sod2 is near the telomere of chromosome I and encodes a plasma membrane Na+(Li+)/H+ antiporter. When sod2 is amplified, S. pombe survives otherwise lethal concentrations of LiCl, and >90% of the amplified sod2 genes are found in 180- and 225-kilobase (kb) linear amplicons. The sequence of the novel joint of the 180-kb amplicon indicates that it is formed by recombination between homologous regions near the telomeres of the long arm of chromosome I and the short arm of chromosome II. The 225-kb amplicon, isolated three times more frequently than the 180-kb amplicon, is a palindrome derived from a region near the telomere of chromosome I. The center of symmetry of this palindrome contains an inverted repeat consisting of two identical 134-base pair sequences separated by a 290-base pair spacer. LiCl-resistant mutants arise 200-600 times more frequently in strains deficient for topoisomerases or DNA ligase activity than in wild-type strains, but the mutant cells contain the same amplicons. These data suggest that amplicon formation may begin with DNA lesions such as breaks. In the case of the 225-kb amplicon, the breaks may lead to a hairpin structure, which is then replicated to form a double-stranded linear amplicon, or to a cruciform structure, which is then resolved to yield the same amplicon.

* Corresponding author. E-mail address: starkg{at}ccf.org.

Molecular Biology of the Cell
Vol. 11, 873-886, March 2000
Copyright © 2000 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 Genes Dev.Home page
D. Branzei and M. Foiani
Leaping forks at inverted repeats
Genes & Dev., January 1, 2010; 24(1): 5 - 9.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A. L. Paek, S. Kaochar, H. Jones, A. Elezaby, L. Shanks, and T. Weinert
Fusion of nearby inverted repeats by a replication-based mechanism leads to formation of dicentric and acentric chromosomes that cause genome instability in budding yeast
Genes & Dev., December 15, 2009; 23(24): 2861 - 2875.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
K. Mizuno, S. Lambert, G. Baldacci, J. M. Murray, and A. M. Carr
Nearby inverted repeats fuse to generate acentric and dicentric palindromic chromosomes by a replication template exchange mechanism
Genes & Dev., December 15, 2009; 23(24): 2876 - 2886.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Tanaka, Y. Cao, D. A. Bergstrom, C. Kooperberg, S. J. Tapscott, and M.-C. Yao
Intrastrand Annealing Leads to the Formation of a Large DNA Palindrome and Determines the Boundaries of Genomic Amplification in Human Cancer
Mol. Cell. Biol., March 15, 2007; 27(6): 1993 - 2002.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. R. Hansen, P. T. Ibarra, and G. Thon
Evolutionary-conserved telomere-linked helicase genes of fission yeast are repressed by silencing factors, RNAi components and the telomere-binding protein Taz1
Nucleic Acids Res., January 10, 2006; 34(1): 78 - 88.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A. J. Rattray, B. K. Shafer, B. Neelam, and J. N. Strathern
A mechanism of palindromic gene amplification in Saccharomyces cerevisiae
Genes & Dev., June 1, 2005; 19(11): 1390 - 1399.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. K. Butler, D. Gillespie, and B. Steele
Formation of Large Palindromic DNA by Homologous Recombination of Short Inverted Repeat Sequences in Saccharomyces cerevisiae
Genetics, July 1, 2002; 161(3): 1065 - 1075.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Tanaka, S. J. Tapscott, B. J. Trask, and M.-C. Yao
Short inverted repeats initiate gene amplification through the formation of a large DNA palindrome in mammalian cells
PNAS, June 25, 2002; 99(13): 8772 - 8777.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]