![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 3, 897-914, March 2000
Department of Physiology, University of Connecticut Health Center,
Farmington, Connecticut 06032
The endoplasmic reticulum (ER) and Golgi were labeled by green
fluorescent protein chimeras and observed by time-lapse confocal microscopy during the rapid cell cycles of sea urchin embryos. The ER
undergoes a cyclical microtubule-dependent accumulation at the mitotic
poles and by photobleaching experiments remains continuous through the
cell cycle. Finger-like indentations of the nuclear envelope near the
mitotic poles appear 2-3 min before the permeability barrier of the
nuclear envelope begins to change. This permeability change in turn is
~30 s before nuclear envelope breakdown. During interphase, there are
many scattered, disconnected Golgi stacks throughout the cytoplasm,
which appear as 1- to 2-µm fluorescent spots. The number of Golgi
spots begins to decline soon after nuclear envelope breakdown, reaches
a minimum soon after cytokinesis, and then rapidly increases. At higher
magnification, smaller spots are seen, along with increased
fluorescence in the ER. Quantitative measurements, along with
nocodazole and photobleaching experiments, are consistent with a
redistribution of some of the Golgi to the ER during mitosis. The
scattered Golgi coalesce into a single large aggregate during the
interphase after the ninth embryonic cleavage; this is likely to be
preparatory for secretion of the hatching enzyme during the following
cleavage cycle.
This article has been cited by other articles:
|
|
 |
|
  L. Lu, M. S. Ladinsky, and T. Kirchhausen Cisternal Organization of the Endoplasmic Reticulum during Mitosis Mol. Biol. Cell, August 1, 2009; 20(15): 3471 - 3480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Teixeira and C. D. Huston Evidence of a Continuous Endoplasmic Reticulum in the Protozoan Parasite Entamoeba histolytica Eukaryot. Cell, July 1, 2008; 7(7): 1222 - 1226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Nakajima, S. Yonemura, M. Murata, N. Nakamura, H. Piwnica-Worms, and E. Nishida Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit J. Cell Biol., April 3, 2008; 181(1): 89 - 103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Puhka, H. Vihinen, M. Joensuu, and E. Jokitalo Endoplasmic reticulum remains continuous and undergoes sheet-to-tubule transformation during cell division in mammalian cells J. Cell Biol., December 3, 2007; 179(5): 895 - 909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Audhya, A. Desai, and K. Oegema A role for Rab5 in structuring the endoplasmic reticulum J. Cell Biol., October 3, 2007; 178(1): 43 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Muhlhausser and U. Kutay An in vitro nuclear disassembly system reveals a role for the RanGTPase system and microtubule-dependent steps in nuclear envelope breakdown J. Cell Biol., August 9, 2007; 178(4): 595 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Browne, J. B. Swan, E. E. Rankin, H. Calvert, S. Griffiths, and M. Tytell Extracellular heat shock protein 70 has novel functional effects on sea urchin eggs and coelomocytes J. Exp. Biol., April 1, 2007; 210(7): 1275 - 1287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Frescas, M. Mavrakis, H. Lorenz, R. DeLotto, and J. Lippincott-Schwartz The secretory membrane system in the Drosophila syncytial blastoderm embryo exists as functionally compartmentalized units around individual nuclei J. Cell Biol., April 24, 2006; 173(2): 219 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Altan-Bonnet, R. Sougrat, W. Liu, E. L. Snapp, T. Ward, and J. Lippincott-Schwartz Golgi Inheritance in Mammalian Cells Is Mediated through Endoplasmic Reticulum Export Activities Mol. Biol. Cell, February 1, 2006; 17(2): 990 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. FitzHarris, M. Larman, C. Richards, and J. Carroll An increase in [Ca2+]i is sufficient but not necessary for driving mitosis in early mouse embryos J. Cell Sci., October 1, 2005; 118(19): 4563 - 4575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Kano, H. Kondo, A. Yamamoto, Y. Kaneko, K. Uchiyama, N. Hosokawa, K. Nagata, and M. Murata NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network Genes Cells, October 1, 2005; 10(10): 989 - 999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Poteryaev, J. M. Squirrell, J. M. Campbell, J. G. White, and A. Spang Involvement of the Actin Cytoskeleton and Homotypic Membrane Fusion in ER Dynamics in Caenorhabditis elegans Mol. Biol. Cell, May 1, 2005; 16(5): 2139 - 2153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Kano, H. Kondo, A. Yamamoto, A. R. Tanaka, N. Hosokawa, K. Nagata, and M. Murata The maintenance of the endoplasmic reticulum network is regulated by p47, a cofactor of p97, through phosphorylation by cdc2 kinase Genes Cells, April 1, 2005; 10(4): 333 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Vedrenne, D. R. Klopfenstein, and H.-P. Hauri Phosphorylation Controls CLIMP-63-mediated Anchoring of the Endoplasmic Reticulum to Microtubules Mol. Biol. Cell, April 1, 2005; 16(4): 1928 - 1937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Uchiyama and H. Kondo p97/p47-Mediated Biogenesis of Golgi and ER J. Biochem., February 1, 2005; 137(2): 115 - 119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Du, S. Ferro-Novick, and P. Novick Dynamics and inheritance of the endoplasmic reticulum J. Cell Sci., June 15, 2004; 117(14): 2871 - 2878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. B. Axelsson and G. Warren Rapid, Endoplasmic Reticulum-independent Diffusion of the Mitotic Golgi Haze Mol. Biol. Cell, April 1, 2004; 15(4): 1843 - 1852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Barr Golgi inheritance: shaken but not stirred J. Cell Biol., March 29, 2004; 164(7): 955 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Andrade, H. Zhao, B. Titus, S. Timm Pearce, and M. Barroso The EF-Hand Ca2+-binding Protein p22 Plays a Role in Microtubule and Endoplasmic Reticulum Organization and Dynamics with Distinct Ca2+-binding Requirements Mol. Biol. Cell, February 1, 2004; 15(2): 481 - 496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Puri and A. D. Linstedt Capacity of the Golgi Apparatus for Biogenesis from the Endoplasmic Reticulum Mol. Biol. Cell, December 1, 2003; 14(12): 5011 - 5018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Terasaki, E.-i. Okumura, B. Hinkle, and T. Kishimoto Localization and Dynamics of Cdc2-Cyclin B during Meiotic Reinitiation in Starfish Oocytes Mol. Biol. Cell, November 1, 2003; 14(11): 4685 - 4694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. FitzHarris, P. Marangos, and J. Carroll Cell Cycle-dependent Regulation of Structure of Endoplasmic Reticulum and Inositol 1,4,5-Trisphosphate-induced Ca2+ Release in Mouse Oocytes and Embryos Mol. Biol. Cell, January 1, 2003; 14(1): 288 - 301. [Abstract] [Full Text]
|
|
|
|
 |
|
 I. J. Dmochowski, J. E. Dmochowski, P. Oliveri, E. H. Davidson, and S. E. Fraser Quantitative imaging of cis-regulatory reporters in living embryos PNAS, October 1, 2002; 99(20): 12895 - 12900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Shuster and D. R. Burgess Targeted new membrane addition in the cleavage furrow is a late, separate event in cytokinesis PNAS, March 19, 2002; 99(6): 3633 - 3638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Seemann, M. Pypaert, T. Taguchi, J. Malsam, and G. Warren Partitioning of the Matrix Fraction of the Golgi Apparatus During Mitosis in Animal Cells Science, February 1, 2002; 295(5556): 848 - 851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Basham and L. S. Rose The Caenorhabditis elegans polarity gene ooc-5 encodes a Torsin-related protein of the AAA ATPase superfamily Development, November 15, 2001; 128(22): 4645 - 4656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. C. de Noronha, M. P. Sherman, H. W. Lin, M. V. Cavrois, R. D. Moir, R. D. Goldman, and W. C. Greene Dynamic Disruptions in Nuclear Envelope Architecture and Integrity Induced by HIV-1 Vpr Science, November 2, 2001; 294(5544): 1105 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Du, M. Pypaert, P. Novick, and S. Ferro-Novick Aux1p/Swa2p Is Required for Cortical Endoplasmic Reticulum Inheritance in Saccharomyces cerevisiae Mol. Biol. Cell, September 1, 2001; 12(9): 2614 - 2628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Jokitalo, N. Cabrera-Poch, G. Warren, and D. T. Shima Golgi clusters and vesicles mediate mitotic inheritance independently of the endoplasmic reticulum J. Cell Biol., July 23, 2001; 154(2): 317 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Lu, D. Joseph, E. Bugnard, K. J. M. Zaal, and E. Ralston Golgi Complex Reorganization during Muscle Differentiation: Visualization in Living Cells and Mechanism Mol. Biol. Cell, April 1, 2001; 12(4): 795 - 808. [Abstract] [Full Text]
|
|
|
|
|
|
M. Terasaki, P. Campagnola, M. M. Rolls, P. A. Stein, J. Ellenberg, B. Hinkle, and B. Slepchenko A New Model for Nuclear Envelope Breakdown Mol. Biol. Cell, February 1, 2001; 12(2): 503 - 510. [Abstract] [Full Text]
|
|
|
|
|
|
K. K. Lee, Y. Gruenbaum, P. Spann, J. Liu, and K. L. Wilson C. elegans Nuclear Envelope Proteins Emerin, MAN1, Lamin, and Nucleoporins Reveal Unique Timing of Nuclear Envelope Breakdown during Mitosis Mol. Biol. Cell, September 1, 2000; 11(9): 3089 - 3099. [Abstract] [Full Text]
|
|
|
|
|
|
W. J. Nelson W(H)ither the Golgi during Mitosis? J. Cell Biol., April 17, 2000; 149(2): 243 - 248. [Full Text] [PDF]
|
|
