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Vol. 11, Issue 4, 1293-1304, April 2000
*Department of Embryology, Howard Hughes Medical Institute,
Carnegie Institution of Washington, Baltimore, Maryland 21210;
and In vitro studies suggest that the Barren protein may function as an
activator of DNA topoisomerase II and/or as a component of the
Xenopus condensin complex. To better understand the role of Barren in vivo, we generated conditional alleles of the structural gene for Barren (BRN1) in Saccharomyces
cerevisiae. We show that Barren is an essential protein
required for chromosome condensation in vivo and that it is likely to
function as an intrinsic component of the yeast condensation machinery.
Consistent with this view, we show that Barren performs an essential
function during a period of the cell cycle when chromosome condensation
is established and maintained. In contrast, Barren does not serve as an
essential activator of DNA topoisomerase II in vivo. Finally,
brn1 mutants display additional phenotypes such as
stretched chromosomes, aberrant anaphase spindles, and the accumulation
of cells with >2C DNA content, suggesting that Barren function
influences multiple aspects of chromosome transmission and dynamics.
Department of Pharmacology and Department of Cellular
and Molecular Medicine, University of California, San Diego, La Jolla,
California 92093-0651.
Corresponding author. E-mail address:
cholm{at}ucsd.edu.
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