Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hettmann, C.
Right arrow Articles by Soldati, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hettmann, C.
Right arrow Articles by Soldati, D.

Vol. 11, Issue 4, 1385-1400, April 2000

A Dibasic Motif in the Tail of a Class XIV Apicomplexan Myosin Is an Essential Determinant of Plasma Membrane Localization

Christine Hettmann,* Angelika Herm,* Ariane Geiter,* Bernd Frank,* Eva Schwarz,dagger Thierry Soldati,dagger and Dominique Soldati*Dagger

 *Zentrum für Molekulare Biologie, Universität Heidelberg, D-69120 Heidelberg, Germany; and dagger Department of Molecular Cell Research, Max-Planck-Institut for Medical Research, D-69120 Heidelberg, Germany

Obligate intracellular parasites of the phylum Apicomplexa exhibit gliding motility, a unique form of substrate-dependent locomotion essential for host cell invasion and shown to involve the parasite actin cytoskeleton and myosin motor(s). Toxoplasma gondii has been shown to express three class XIV myosins, TgM-A, -B, and -C. We identified an additional such myosin, TgM-D, and completed the sequences of a related Plasmodium falciparum myosin, PfM-A. Despite divergent structural features, TgM-A purified from parasites bound actin in an ATP-dependent manner. Isoform-specific antibodies revealed that TgM-A and recombinant mycTgM-A were localized right beneath the plasma membrane, and subcellular fractionation indicated a tight membrane association. Recombinant TgM-D also had a peripheral although not as sharply defined localization. Truncation of their respective tail domains abolished peripheral localization and tight membrane association. Conversely, fusion of the tails to green fluorescent protein (GFP) was sufficient to confer plasma membrane localization and sedimentability. The peripheral localization of TgM-A and of the GFP-tail fusion did not depend on an intact F-actin cytoskeleton, and the GFP chimera did not localize to the plasma membrane of HeLa cells. Finally, we showed that the specific localization determinants were in the very C terminus of the TgM-A tail, and site-directed mutagenesis revealed two essential arginine residues. We discuss the evidence for a proteinaceous plasma membrane receptor and the implications for the invasion process.

Dagger Corresponding author. E-mail address: soldati{at}sun0.urz.uni-heidelberg.de.

Molecular Biology of the Cell
Vol. 11, 1385-1400, April 2000
Copyright © 2000 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Bosch, S. Turley, T. M. Daly, S. M. Bogh, M. L. Villasmil, C. Roach, N. Zhou, J. M. Morrisey, A. B. Vaidya, L. W. Bergman, et al.
Structure of the MTIP-MyoA complex, a key component of the malaria parasite invasion motor
PNAS, March 28, 2006; 103(13): 4852 - 4857.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. J. Foth, M. C. Goedecke, and D. Soldati
From the Cover: New insights into myosin evolution and classification
PNAS, March 7, 2006; 103(10): 3681 - 3686.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Baum, D. Richard, J. Healer, M. Rug, Z. Krnajski, T.-W. Gilberger, J. L. Green, A. A. Holder, and A. F. Cowman
A Conserved Molecular Motor Drives Cell Invasion and Gliding Motility across Malaria Life Cycle Stages and Other Apicomplexan Parasites
J. Biol. Chem., February 24, 2006; 281(8): 5197 - 5208.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
E. Gaskins, S. Gilk, N. DeVore, T. Mann, G. Ward, and C. Beckers
Identification of the membrane receptor of a class XIV myosin in Toxoplasma gondii
J. Cell Biol., May 10, 2004; 165(3): 383 - 393.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
L. D. Sibley
Intracellular Parasite Invasion Strategies
Science, April 9, 2004; 304(5668): 248 - 253.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
L. Li, B. P. Brunk, J. C. Kissinger, D. Pape, K. Tang, R. H. Cole, J. Martin, T. Wylie, M. Dante, S. J. Fogarty, et al.
Gene Discovery in the Apicomplexa as Revealed by EST Sequencing and Assembly of a Comparative Gene Database
Genome Res., March 1, 2003; 13(3): 443 - 454.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L.-Y. Kwok, S. Lutjen, S. Soltek, D. Soldati, D. Busch, M. Deckert, and D. Schluter
The Induction and Kinetics of Antigen-Specific CD8 T Cells Are Defined by the Stage Specificity and Compartmentalization of the Antigen in Murine Toxoplasmosis
J. Immunol., February 15, 2003; 170(4): 1949 - 1957.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D.M. Wetzel, S. Hakansson, K. Hu, D. Roos, and L.D. Sibley
Actin Filament Polymerization Regulates Gliding Motility by Apicomplexan Parasites
Mol. Biol. Cell, February 1, 2003; 14(2): 396 - 406.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. W. Bergman, K. Kaiser, H. Fujioka, I. Coppens, T. M. Daly, S. Fox, K. Matuschewski, V. Nussenzweig, and S. H. I. Kappe
Myosin A tail domain interacting protein (MTIP) localizes to the inner membrane complex of Plasmodium sporozoites
J. Cell Sci., January 1, 2003; 116(1): 39 - 49.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. Meissner, D. Schluter, and D. Soldati
Role of Toxoplasma gondii Myosin A in Powering Parasite Gliding and Host Cell Invasion
Science, October 25, 2002; 298(5594): 837 - 840.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
N. S. Morrissette and L. D. Sibley
Cytoskeleton of Apicomplexan Parasites
Microbiol. Mol. Biol. Rev., March 1, 2002; 66(1): 21 - 38.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Meissner, M. Reiss, N. Viebig, V. B. Carruthers, C. Toursel, S. Tomavo, J. W. Ajioka, and D. Soldati
A family of transmembrane microneme proteins of Toxoplasma gondii contain EGF-like domains and function as escorters
J. Cell Sci., January 2, 2002; 115(3): 563 - 574.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Meissner, S. Brecht, H. Bujard, and D. Soldati
Modulation of myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii
Nucleic Acids Res., November 15, 2001; 29(22): e115 - e115.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
F. Delbac, A. Sanger, E. M. Neuhaus, R. Stratmann, J. W. Ajioka, C. Toursel, A. Herm-Gotz, S. Tomavo, T. Soldati, and D. Soldati
Toxoplasma gondii myosins B/C: one gene, two tails, two localizations, and a role in parasite division
J. Cell Biol., November 12, 2001; 155(4): 613 - 624.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Brecht, V. B. Carruthers, D. J. P. Ferguson, O. K. Giddings, G. Wang, U. Jakle, J. M. Harper, L. D. Sibley, and D. Soldati
The Toxoplasma Micronemal Protein MIC4 Is an Adhesin Composed of Six Conserved Apple Domains
J. Biol. Chem., February 2, 2001; 276(6): 4119 - 4127.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]