![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 5, 1509-1521, May 2000
and
*Department of Biomedical Sciences, Ohio University College of
Osteopathic Medicine, Athens, Ohio 45701; and
The chloride intracellular channel (CLIC) gene family has been
implicated in chloride ion transport within various subcellular compartments. We report here the molecular, biochemical, and cellular characterization of a new member of this gene family termed CLIC5. CLIC5 was isolated from extracts of placental microvilli as a component
of a multimeric complex consisting of several known cytoskeletal
proteins, including actin, ezrin, Department of Molecular Biology and Genetics, Cornell
University, Ithaca, New York 14853
-actinin, gelsolin, and IQGAP1. We
cloned human cDNAs and generated antibodies specific for CLIC5,
CLIC1/NCC27, and CLIC4/huH1/p64H1. CLIC5 shares 52-76% overall
identity with human CLIC1, CLIC2, CLIC3, and CLIC4. Northern blot
analysis showed that CLIC5 has a distinct pattern of expression compared with CLIC1 and CLIC4. Immunoblot analysis of
extracts from placental tissues demonstrated that CLIC4 and CLIC5 are
enriched in isolated placental microvilli, whereas CLIC1 is not.
Moreover, in contrast to CLIC1 and CLIC4, CLIC5 is associated with the
detergent-insoluble cytoskeletal fraction of microvilli. Indirect
immunofluorescence microscopy revealed that CLIC4 and CLIC5 are
concentrated within the apical region of the trophoblast, whereas CLIC1
is distributed throughout the cytoplasm. These studies suggest that
CLIC1, CLIC4, and CLIC5 play distinct roles in chloride transport and
that CLIC5 interacts with the cortical actin cytoskeleton in polarized
epithelial cells.
Corresponding author. E-mail address:
berryman{at}ohiou.edu.
This article has been cited by other articles:
|
|
 |
|
  A. Elter, A. Hartel, C. Sieben, B. Hertel, E. Fischer-Schliebs, U. Luttge, A. Moroni, and G. Thiel A Plant Homolog of Animal Chloride Intracellular Channels (CLICs) Generates an Ion Conductance in Heterologous Systems J. Biol. Chem., March 23, 2007; 282(12): 8786 - 8792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. H. Gagnon, C. M. Longo-Guess, M. Berryman, J.-B. Shin, K. W. Saylor, H. Yu, P. G. Gillespie, and K. R. Johnson The Chloride Intracellular Channel Protein CLIC5 Is Expressed at High Levels in Hair Cell Stereocilia and Is Essential for Normal Inner Ear Function J. Neurosci., October 4, 2006; 26(40): 10188 - 10198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Montalbetti, Q. Li, G. A Timpanaro, S. Gonzalez-Perrett, X.-Q. Dai, X.-Z. Chen, and H. F Cantiello Cytoskeletal regulation of calcium-permeable cation channels in the human syncytiotrophoblast: role of gelsolin J. Physiol., July 15, 2005; 566(2): 309 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Chew, C. T. Okamoto, X. Chen, and H. Y. Qin IQGAPs are differentially expressed and regulated in polarized gastric epithelial cells Am J Physiol Gastrointest Liver Physiol, February 1, 2005; 288(2): G376 - G387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Suh, M. Mutoh, M. Gerdes, J. M. Crutchley, T. Mutoh, L. E. Edwards, R. A. Dumont, P. Sodha, C. Cheng, A. Glick,, et al. Antisense Suppression of the Chloride Intracellular Channel Family Induces Apoptosis, Enhances Tumor Necrosis Factor {alpha}-Induced Apoptosis, and Inhibits Tumor Growth Cancer Res., January 15, 2005; 65(2): 562 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Berryman, J. Bruno, J. Price, and J. C. Edwards CLIC-5A Functions as a Chloride Channel in Vitro and Associates with the Cortical Actin Cytoskeleton in Vitro and in Vivo J. Biol. Chem., August 13, 2004; 279(33): 34794 - 34801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Shanks, M. C. Larocca, M. Berryman, J. C. Edwards, T. Urushidani, J. Navarre, and J. R. Goldenring AKAP350 at the Golgi Apparatus. II. ASSOCIATION OF AKAP350 WITH A NOVEL CHLORIDE INTRACELLULAR CHANNEL (CLIC) FAMILY MEMBER J. Biol. Chem., October 18, 2002; 277(43): 40973 - 40980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Thevenod Ion channels in secretory granules of the pancreas and their role in exocytosis and release of secretory proteins Am J Physiol Cell Physiol, September 1, 2002; 283(3): C651 - C672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ronnov-Jessen, R. Villadsen, J. C. Edwards, and O. W. Petersen Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-{beta}1-Mediated Conversion of Fibroblasts to Myofibroblasts Am. J. Pathol., August 1, 2002; 161(2): 471 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Thompson, M. H. Nordeen, K. E. Howell, and J. H. Caldwell A Large-Conductance Anion Channel of the Golgi Complex Biophys. J., July 1, 2002; 83(1): 278 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Fernandez-Salas, K. S. Suh, V. V. Speransky, W. L. Bowers, J. M. Levy, T. Adams, K. R. Pathak, L. E. Edwards, D. D. Hayes, C. Cheng, et al. mtCLIC/CLIC4, an Organellular Chloride Channel Protein, Is Increased by DNA Damage and Participates in the Apoptotic Response to p53 Mol. Cell. Biol., June 1, 2002; 22(11): 3610 - 3620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik Molecular Structure and Physiological Function of Chloride Channels Physiol Rev, April 1, 2002; 82(2): 503 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Lange Role of microvillar cell surfaces in the regulation of glucose uptake and organization of energy metabolism Am J Physiol Cell Physiol, January 1, 2002; 282(1): C1 - C26. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Reczek and A. Bretscher Identification of EPI64, a TBC/rabGAP Domain-containing Microvillar Protein That Binds to the First PDZ Domain of EBP50 and E3KARP J. Cell Biol., April 2, 2001; 153(1): 191 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Harrop, M. Z. DeMaere, W. D. Fairlie, T. Reztsova, S. M. Valenzuela, M. Mazzanti, R. Tonini, M. R. Qiu, L. Jankova, K. Warton, et al. Crystal Structure of a Soluble Form of the Intracellular Chloride Ion Channel CLIC1 (NCC27) at 1.4-A Resolution J. Biol. Chem., November 21, 2001; 276(48): 44993 - 45000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mizukawa, T. Nishizawa, T. Nagao, K. Kitamura, and T. Urushidani Cellular distribution of parchorin, a chloride intracellular channel-related protein, in various tissues Am J Physiol Cell Physiol, April 1, 2002; 282(4): C786 - C795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Tulk, S. Kapadia, and J. C. Edwards CLIC1 inserts from the aqueous phase into phospholipid membranes, where it functions as an anion channel Am J Physiol Cell Physiol, May 1, 2002; 282(5): C1103 - C1112. [Abstract] [Full Text] [PDF]
|
|
