![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 5, 1815-1827, May 2000
and§
This study was undertaken to obtain direct evidence for the
involvement of gap junctions in the propagation of intercellular Ca2+ waves. Gap junction-deficient HeLa cells were
transfected with plasmids encoding for green fluorescent protein (GFP)
fused to the cytoplasmic carboxyl termini of connexin 43 (Cx43), 32 (Cx32), or 26 (Cx26). The subsequently expressed GFP-labeled gap
junctions rendered the cells dye- and electrically coupled and were
detected at the plasma membranes at points of contact between adjacent cells. To correlate the distribution of gap junctions with the changes
in [Ca2+]i associated with Ca2+
waves and the distribution of the endoplasmic reticulum (ER), cells
were loaded with fluorescent Ca2+-sensitive (fluo-3 and
fura-2) and ER membrane (ER-Tracker) dyes. Digital high-speed
microscopy was used to collect a series of image slices from which the
three-dimensional distribution of the gap junctions and ER were
reconstructed. Subsequently, intercellular Ca2+ waves were
induced in these cells by mechanical stimulation with or without
extracellular apyrase, an ATP-degrading enzyme. In untransfected HeLa
cells and in the absence of apyrase, cell-to-cell propagating
[Ca2+]i changes were characterized by
initiating Ca2+ puffs associated with the perinuclear ER.
By contrast, in Cx-GFP-transfected cells and in the presence of
apyrase, [Ca2+]i changes were propagated
without initiating perinuclear Ca2+ puffs and were
communicated between cells at the sites of the Cx-GFP gap junctions.
The efficiency of Cx expression determined the extent of
Ca2+ wave propagation. These results demonstrate that
intercellular Ca2+ waves may be propagated simultaneously
via an extracellular pathway and an intracellular pathway through gap
junctions and that one form of communication may mask the other.
Department of Physiology, University of
Massachusetts Medical School, Worcester, Massachusetts 01655;
*Department of Physiology and Pathophysiology, University of Ghent,
B-9000 Ghent, Belgium; and Department of Medical
Biochemistry, University of Wales College of Medicine, Cardiff CF4 4XN,
Wales, United Kingdom
This article has been cited by other articles:
|
|
 |
|
  M. M. Lurtz and C. F. Louis Intracellular calcium regulation of connexin43 Am J Physiol Cell Physiol, December 1, 2007; 293(6): C1806 - C1813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Q. Gong, Q. Shao, S. Langlois, D. Bai, and D. W. Laird Differential Potency of Dominant Negative Connexin43 Mutants in Oculodentodigital Dysplasia J. Biol. Chem., June 29, 2007; 282(26): 19190 - 19202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Bootman, D. R. Higazi, S. Coombes, and H. L. Roderick Calcium signalling during excitation-contraction coupling in mammalian atrial myocytes. J. Cell Sci., October 1, 2006; 119(Pt 19): 3915 - 3925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Onfelt, M. A. Purbhoo, S. Nedvetzki, S. Sowinski, and D. M. Davis Long-Distance Calls Between Cells Connected by Tunneling Nanotubules Sci. Signal., December 6, 2005; 2005(313): pe55 - pe55. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Richter-Unruh, M. Verhoef-Post, S. Malak, J. Homoki, B. P. Hauffa, and A. P. N. Themmen Leydig Cell Hypoplasia: Absent Luteinizing Hormone Receptor Cell Surface Expression Caused by a Novel Homozygous Mutation in the Extracellular Domain J. Clin. Endocrinol. Metab., October 1, 2004; 89(10): 5161 - 5167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Boitano, Z. Safdar, D. G. Welsh, J. Bhattacharya, and M. Koval Cell-cell interactions in regulating lung function Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L455 - L459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E.M. Martin and W.H. Evans Incorporation of connexins into plasma membranes and gap junctions Cardiovasc Res, May 1, 2004; 62(2): 378 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Lin, J. K. Rurangirwa, M. Koval, and T. H. Steinberg Gap junctional communication modulates agonist-induced calcium oscillations in transfected HeLa cells J. Cell Sci., February 22, 2004; 117(6): 881 - 887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. K. Marziano, S. O. Casalotti, A. E. Portelli, D. L. Becker, and A. Forge Mutations in the gene for connexin 26 (GJB2) that cause hearing loss have a dominant negative effect on connexin 30 Hum. Mol. Genet., April 15, 2003; 12(8): 805 - 812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Kindzelskii and H. R. Petty Intracellular Calcium Waves Accompany Neutrophil Polarization, Formylmethionylleucylphenylalanine Stimulation, and Phagocytosis: A High Speed Microscopy Study J. Immunol., January 1, 2003; 170(1): 64 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koval Sharing signals: connecting lung epithelial cells with gap junction channels Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L875 - L893. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Oviedo-Orta and W. H. Evans Gap junctions and connexins: potential contributors to the immunological synapse J. Leukoc. Biol., October 1, 2002; 72(4): 636 - 642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Ashby and A. V. Tepikin Polarized Calcium and Calmodulin Signaling in Secretory Epithelia Physiol Rev, July 1, 2002; 82(3): 701 - 734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hofer, L. Venance, and C. Giaume Control and Plasticity of Intercellular Calcium Waves in Astrocytes: A Modeling Approach J. Neurosci., June 15, 2002; 22(12): 4850 - 4859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Leite, K. Hirata, T. Pusl, A. D. Burgstahler, K. Okazaki, J. M. Ortega, A. M. Goes, M. A. M. Prado, D. C. Spray, and M. H. Nathanson Molecular Basis for Pacemaker Cells in Epithelia J. Biol. Chem., May 3, 2002; 277(18): 16313 - 16323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Lawe, A. Chawla, E. Merithew, J. Dumas, W. Carrington, K. Fogarty, L. Lifshitz, R. Tuft, D. Lambright, and S. Corvera Sequential Roles for Phosphatidylinositol 3-Phosphate and Rab5 in Tethering and Fusion of Early Endosomes via Their Interaction with EEA1 J. Biol. Chem., March 1, 2002; 277(10): 8611 - 8617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Isakson, R. L. Lubman, G. J. Seedorf, and S. Boitano Modulation of pulmonary alveolar type II cell phenotype and communication by extracellular matrix and KGF Am J Physiol Cell Physiol, October 1, 2001; 281(4): C1291 - C1299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. T. Chaytor, P. E. M. Martin, D. H. Edwards, and T. M. Griffith Gap junctional communication underpins EDHF-type relaxations evoked by ACh in the rat hepatic artery Am J Physiol Heart Circ Physiol, June 1, 2001; 280(6): H2441 - H2450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Baruch, D. Greenbaum, E. T. Levy, P. A. Nielsen, N. B. Gilula, N. M. Kumar, and M. Bogyo Defining a Link between Gap Junction Communication, Proteolysis, and Cataract Formation J. Biol. Chem., July 27, 2001; 276(31): 28999 - 29006. [Abstract] [Full Text] [PDF]
|
|
