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Vol. 11, Issue 5, 1905-1917, May 2000
School of Biological Sciences, University of Manchester, Manchester
M13 9PT, United Kingdom
A novel selection scheme has been developed to isolate bloodstream
forms of Trypanosoma brucei, which are defective in
their ability to differentiate to the procyclic stage. Detailed
characterization of one selected cell line (defective in
differentiation clone 1 [DiD-1]) has demonstrated that these cells
are indistinguishable from the wild-type population in terms of their
morphology, cell cycle progression, and biochemical characteristics but
are defective in their ability to initiate differentiation to the
procyclic form. Although a small proportion of DiD-1 cells remain able
to transform, deletion of the genes for glycophosphatidyl
inositol-phospholipase C demonstrated that this enzyme was not
responsible for this inefficient differentiation. However, the
attenuated growth of the 
-glycophosphatidyl inositol-phospholipase C DiD-1 cells in mice permitted
the expression of stumpy characteristics in this previously monomorphic
cell line, and concomitantly their ability to differentiate efficiently was restored. Our results indicate that monomorphic cells retain expression of a characteristic of the stumpy form essential for differentiation, and that this is reduced in the defective cells. This
approach provides a new route to dissection of the cytological and
molecular basis of life cycle progression in the African trypanosome.
This article has been cited by other articles:
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  S. Laxman, A. Riechers, M. Sadilek, F. Schwede, and J. A. Beavo Hydrolysis products of cAMP analogs cause transformation of Trypanosoma brucei from slender to stumpy-like forms PNAS, December 12, 2006; 103(50): 19194 - 19199. [Abstract] [Full Text] [PDF]
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 B. Szoor, J. Wilson, H. McElhinney, L. Tabernero, and K. R. Matthews Protein tyrosine phosphatase TbPTP1: a molecular switch controlling life cycle differentiation in trypanosomes J. Cell Biol., October 23, 2006; 175(2): 293 - 303. [Abstract] [Full Text] [PDF]
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 M. Engstler and M. Boshart Cold shock and regulation of surface protein trafficking convey sensitization to inducers of stage differentiation in Trypanosoma brucei Genes & Dev., November 15, 2004; 18(22): 2798 - 2811. [Abstract] [Full Text] [PDF]
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 E. F. Hendriks, A. Abdul-Razak, and K. R. Matthews tbCPSF30 Depletion by RNA Interference Disrupts Polycistronic RNA Processing in Trypanosoma brucei J. Biol. Chem., July 11, 2003; 278(29): 26870 - 26878. [Abstract] [Full Text] [PDF]
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 I. B. Muller, D. Domenicali-Pfister, I. Roditi, and E. Vassella Stage-specific Requirement of a Mitogen-activated Protein Kinase by Trypanosoma brucei Mol. Biol. Cell, November 1, 2002; 13(11): 3787 - 3799. [Abstract] [Full Text] [PDF]
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 L. Quijada, C. Guerra-Giraldez, M. Drozdz, C. Hartmann, H. Irmer, C. Ben-Dov, M. Cristodero, M. Ding, and C. Clayton Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements Nucleic Acids Res., October 15, 2002; 30(20): 4414 - 4424. [Abstract] [Full Text] [PDF]
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M. W. Timms, F. J. van Deursen, E. F. Hendriks, and K. R. Matthews Mitochondrial Development during Life Cycle Differentiation of African Trypanosomes: Evidence for a Kinetoplast-dependent Differentiation Control Point Mol. Biol. Cell, October 1, 2002; 13(10): 3747 - 3759. [Abstract] [Full Text] [PDF]
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