Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Trumtel, S.
Right arrow Articles by Gas, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trumtel, S.
Right arrow Articles by Gas, N.

Vol. 11, Issue 6, 2175-2189, June 2000

Assembly and Functional Organization of the Nucleolus: Ultrastructural Analysis of Saccharomyces cerevisiae Mutants

Stéphanie Trumtel, Isabelle Léger-Silvestre,* Pierre-Emmanuel Gleizes, Frédéric Teulières, and Nicole Gas

Laboratoire de Biologie Moléculaire Eucaryote, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5099, and Université Paul Sabatier, 31062 Toulouse Cedex, France

Using Saccharomyces cerevisiae strains with genetically modified nucleoli, we show here that changing parameters as critical as the tandem organization of the ribosomal genes and the polymerase transcribing rDNA, although profoundly modifying the position and the shape of the nucleolus, only partially alter its functional subcompartmentation. High-resolution morphology achieved by cryofixation, together with ultrastructural localization of nucleolar proteins and rRNA, reveals that the nucleolar structure, arising upon transcription of rDNA from plasmids by RNA polymerase I, is still divided in functional subcompartments like the wild-type nucleolus. rRNA maturation is restricted to a fibrillar component, reminiscent of the dense fibrillar component in wild-type cells; a granular component is also present, whereas no fibrillar center can be distinguished, which directly links this latter substructure to rDNA chromosomal organization. Although morphologically different, the mininucleoli observed in cells transcribing rDNA with RNA polymerase II also contain a fibrillar subregion of analogous function, in addition to a dense core of unknown nature. Upon repression of rDNA transcription in this strain or in an RNA polymerase I thermosensitive mutant, the nucleolar structure falls apart (in a reversible manner), and nucleolar constituents partially relocate to the nucleoplasm, indicating that rRNA is a primary determinant for the assembly of the nucleolus.

* Corresponding author. E-mail address: leger{at}ibcg.biotoul.fr.

Molecular Biology of the Cell
Vol. 11, 2175-2189, June 2000
Copyright © 2000 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 RNAHome page
R. Thomsen, C. Saguez, T. Nasser, and T. H. Jensen
General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutants
RNA, April 1, 2008; 14(4): 706 - 716.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Carneiro, C. Carvalho, J. Braga, J. Rino, L. Milligan, D. Tollervey, and M. Carmo-Fonseca
Inactivation of Cleavage Factor I Components Rna14p and Rna15p Induces Sequestration of Small Nucleolar Ribonucleoproteins at Discrete Sites in the Nucleus
Mol. Biol. Cell, April 1, 2008; 19(4): 1499 - 1508.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Carneiro, C. Carvalho, J. Braga, J. Rino, L. Milligan, D. Tollervey, and M. Carmo-Fonseca
Depletion of the Yeast Nuclear Exosome Subunit Rrp6 Results in Accumulation of Polyadenylated RNAs in a Discrete Domain within the Nucleolus
Mol. Cell. Biol., June 1, 2007; 27(11): 4157 - 4165.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Binnie, P. Castelo-Branco, J. Monks, and N. J. Proudfoot
Homologous gene sequences mediate transcription-domain formation
J. Cell Sci., September 15, 2006; 119(18): 3876 - 3887.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
K. Belaya, D. Tollervey, and M. Kos
FLIPing heterokaryons to analyze nucleo-cytoplasmic shuttling of yeast proteins
RNA, May 1, 2006; 12(5): 921 - 930.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
X. Du, M. R.K. S. Rao, X. Q. Chen, W. Wu, S. Mahalingam, and D. Balasundaram
The Homologous Putative GTPases Grn1p from Fission Yeast and the Human GNL3L Are Required for Growth and Play a Role in Processing of Nucleolar Pre-rRNA
Mol. Biol. Cell, January 1, 2006; 17(1): 460 - 474.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Z. Win, H. W. Mankouri, I. D. Hickson, and S.-W. Wang
A role for the fission yeast Rqh1 helicase in chromosome segregation
J. Cell Sci., December 15, 2005; 118(24): 5777 - 5784.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
A. K. HENRAS, E. BERTRAND, and G. CHANFREAU
A cotranscriptional model for 3'-end processing of the Saccharomyces cerevisiae pre-ribosomal RNA precursor
RNA, October 20, 2004; 10(10): 1572 - 1585.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
T. Naryshkina, A. Bruning, O. Gadal, and K. Severinov
Role of Second-Largest RNA Polymerase I Subunit Zn-Binding Domain in Enzyme Assembly
Eukaryot. Cell, October 1, 2003; 2(5): 1046 - 1052.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
M. NOMURA
Ribosomal RNA Genes, RNA Polymerases, Nucleolar Structures, and Synthesis of rRNA in the Yeast Saccharomyces cerevisiae
Cold Spring Harb Symp Quant Biol, January 1, 2001; 66(0): 555 - 566.
[Abstract] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]