![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 7, 2201-2211, July 2000
and
*Department of Cell Biology and Institute of Biomembranes, Utrecht
University Medical Center, 3584 CX Utrecht, The Netherlands, and
Transport through the endocytic pathway is inhibited during
mitosis. The mechanism responsible for this inhibition is not understood. Rab4 might be one of the proteins involved as it regulates transport through early endosomes, is phosphorylated by
p34cdc2 kinase, and is translocated from early endosomes to
the cytoplasm during mitosis. We investigated the perturbation of the
rab4 GTPase cycle during mitosis. Newly synthesized rab4 was less
efficiently targeted to membranes during mitosis. By subcellular
fractionation of mitotic cells, we found a large increase of cytosolic
rab4 in the active GTP-form, an increase not associated with the
cytosolic rabGDP chaperone GDI. Instead, phosphorylated rab4 is in a
complex with the peptidyl-prolyl isomerase Pin1 during mitosis, but not during interphase. Our results show that less efficient recruitment of
rab4 to membranes and a bypass of the normal GDI-mediated retrieval of
rab4GDP from early endosomes reduce the amount of rab4GTP on membranes
during mitosis. We propose that phosphorylation of rab4 inhibits both
the recruitment of rab4 effector proteins to early endosomes and the
docking of rab4-containing transport vesicles. This mechanism
might contribute to the inhibition of endocytic membrane transport
during mitosis.
Cancer Biology Program, Department of Medicine, Beth
Israel Deaconess Medical Center, and Division on Aging, Harvard Medical
School, Boston, Massachusetts 02215
Corresponding author. E-mail
address: pvander{at}knoware.nl.
This article has been cited by other articles:
|
|
 |
|
  C. Fila, C. Metz, and P. van der Sluijs Juglone Inactivates Cysteine-rich Proteins Required for Progression through Mitosis J. Biol. Chem., August 1, 2008; 283(31): 21714 - 21724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Ahmed, B. C. Jeffus, S. I. A. Bukhari, J. T. Harney, R. Unal, V. V. Lupashin, P. van der Sluijs, and F. Kilic Serotonin Transamidates Rab4 and Facilitates Its Binding to the C Terminus of Serotonin Transporter J. Biol. Chem., April 4, 2008; 283(14): 9388 - 9398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Krawczyk, E. Leimgruber, Q. Seguin-Estevez, I. Dunand-Sauthier, E. Barras, and W. Reith Expression of RAB4B, a protein governing endocytic recycling, is co-regulated with MHC class II genes Nucleic Acids Res., January 28, 2007; 35(2): 595 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Fouraux, M. Deneka, V. Ivan, A. van der Heijden, J. Raymackers, D. van Suylekom, W. J. van Venrooij, P. van der Sluijs, and G. J.M. Pruijn rabip4' Is an Effector of rab5 and rab4 and Regulates Transport through Early Endosomes Mol. Biol. Cell, February 1, 2004; 15(2): 611 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Chen, S. A. Ernst, and J. A. Williams Dominant Negative Rab3D Mutants Reduce GTP-bound Endogenous Rab3D in Pancreatic Acini J. Biol. Chem., December 12, 2003; 278(50): 50053 - 50060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ryo, Y.-C. Liou, K. P. Lu, and G. Wulf Prolyl isomerase Pin1: a catalyst for oncogenesis and a potential therapeutic target in cancer J. Cell Sci., March 1, 2003; 116(5): 773 - 783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mohrmann, L. Gerez, V. Oorschot, J. Klumperman, and P. van der Sluijs rab4 Function in Membrane Recycling from Early Endosomes Depends on a Membrane to Cytoplasm Cycle J. Biol. Chem., August 23, 2002; 277(35): 32029 - 32035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Govers, P. van der Sluijs, E. van Donselaar, J.-W. Slot, and T. J. Rabelink Endothelial Nitric Oxide Synthase and Its Negative Regulator Caveolin-1 Localize to Distinct Perinuclear Organelles J. Histochem. Cytochem., June 1, 2002; 50(6): 779 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mohrmann, R. Leijendekker, L. Gerez, and P. van der Sluijs rab4 Regulates Transport to the Apical Plasma Membrane in Madin-Darby Canine Kidney Cells J. Biol. Chem., March 15, 2002; 277(12): 10474 - 10481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-k. Huang, S. L. Forsburg, U. P. John, M. J. O'Connell, and T. Hunter Isolation and characterization of the Pin1/Ess1p homologue in Schizosaccharomyces pombe J. Cell Sci., March 12, 2002; 114(20): 3779 - 3788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-C. Liou, A. Ryo, H.-K. Huang, P.-J. Lu, R. Bronson, F. Fujimori, T. Uchida, T. Hunter, and K. P. Lu Loss of Pin1 function in the mouse causes phenotypes resembling cyclin D1-null phenotypes PNAS, January 17, 2002; (2002) 32404099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. He, A. G. Lau, M. B. Yaffe, and R. A. Hall Phosphorylation and Cell Cycle-dependent Regulation of Na+/H+ Exchanger Regulatory Factor-1 by Cdc2 Kinase J. Biol. Chem., November 2, 2001; 276(45): 41559 - 41565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Li, W. Omata, I. Kojima, and H. Shibata Direct Interaction of Rab4 with Syntaxin 4 J. Biol. Chem., February 9, 2001; 276(7): 5265 - 5273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Liou, A. Ryo, H.-K. Huang, P.-J. Lu, R. Bronson, F. Fujimori, T. Uchida, T. Hunter, and K. P. Lu Loss of Pin1 function in the mouse causes phenotypes resembling cyclin D1-null phenotypes PNAS, February 5, 2002; 99(3): 1335 - 1340. [Abstract] [Full Text] [PDF]
|
|
