![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 7, 2315-2325, July 2000
§ and
*Molecular Biology and Virology Laboratory, The Salk Institute,
10010 North Torrey Pines Road, La Jolla, California 92037;
Polyubiquitination marks proteins for degradation by the 26S
proteasome and is carried out by a cascade of enzymes that includes ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). The anaphase-promoting complex or
cyclosome (APC/C) comprises a multisubunit ubiquitin ligase that
mediates mitotic progression. Here, we provide evidence that the
Saccharomyces cerevisiae RING-H2 finger protein Apc11
defines the minimal ubiquitin ligase activity of the APC. We found that the integrity of the Apc11p RING-H2 finger was essential for budding yeast cell viability, Using purified, recombinant proteins we showed
that Apc11p interacted directly with the Ubc4 ubiquitin conjugating
enzyme (E2). Furthermore, purified Apc11p was capable of mediating E1-
and E2-dependent ubiquitination of protein substrates, including Clb2p,
in vitro. The ability of Apc11p to act as an E3 was dependent on the
integrity of the RING-H2 finger, but did not require the presence of
the cullin-like APC subunit Apc2p. We suggest that Apc11p is
responsible for recruiting E2s to the APC and for mediating the
subsequent transfer of ubiquitin to APC substrates in vivo.
Center for Molecular Medicine and Therapeutics,
University of British Columbia, Vancouver, British Columbia V5Z4H4; and
§Graduate Program in Biochemistry, Cellular and Molecular
Biology, The Johns Hopkins School of Medicine, Baltimore, Maryland
21205
C.A.P.J. and A.M.P. contributed equally to
this work.
Corresponding author. E-mail address: hunter{at}salk.edu.
This article has been cited by other articles:
|
|
 |
|
  S. Nam, K. Min, H. Hwang, H.-o. Lee, J. H. Lee, J. Yoon, H. Lee, S. Park, and J. Lee Control of Rapsyn Stability by the CUL-3-containing E3 Ligase Complex J. Biol. Chem., March 20, 2009; 284(12): 8195 - 8206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pal, O. Nagy, D. Menesi, A. Udvardy, and P. Deak Structurally related TPR subunits contribute differently to the function of the anaphase-promoting complex in Drosophila melanogaster J. Cell Sci., September 15, 2007; 120(18): 3238 - 3248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Yi and M. D. Ehlers Emerging Roles for Ubiquitin and Protein Degradation in Neuronal Function Pharmacol. Rev., March 1, 2007; 59(1): 14 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Thornton and D. P. Toczyski Precise destruction: an emerging picture of the APC. Genes & Dev., November 15, 2006; 20(22): 3069 - 3078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamada, J. Ohnishi, B. Ohkawara, S. Iemura, K. Satoh, J. Hyodo-Miura, K. Kawachi, T. Natsume, and H. Shibuya NARF, an Nemo-like Kinase (NLK)-associated Ring Finger Protein Regulates the Ubiquitylation and Degradation of T Cell Factor/Lymphoid Enhancer Factor (TCF/LEF) J. Biol. Chem., July 28, 2006; 281(30): 20749 - 20760. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Afonso, E. R. Tulman, G. Delhon, Z. Lu, G. J. Viljoen, D. B. Wallace, G. F. Kutish, and D. L. Rock Genome of crocodilepox virus. J. Virol., May 1, 2006; 80(10): 4978 - 4991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Thornton, T. M. Ng, M. E. Matyskiela, C. W. Carroll, D. O. Morgan, and D. P. Toczyski An architectural map of the anaphase-promoting complex. Genes & Dev., February 15, 2006; 20(4): 449 - 460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ozkan, H. Yu, and J. Deisenhofer Mechanistic insight into the allosteric activation of a ubiquitin-conjugating enzyme by RING-type ubiquitin ligases PNAS, December 27, 2005; 102(52): 18890 - 18895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Jenkins, V. Markovtsov, W. Lang, P. Sharma, D. Pearsall, J. Warner, C. Franci, B. Huang, J. Huang, G. C. Yam, et al. Critical Role of the Ubiquitin Ligase Activity of UHRF1, a Nuclear RING Finger Protein, in Tumor Cell Growth Mol. Biol. Cell, December 1, 2005; 16(12): 5621 - 5629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Page, V. Aneliunas, J. R. Lamb, and P. Hieter In Vivo Characterization of the Nonessential Budding Yeast Anaphase-Promoting Complex/Cyclosome Components Swm1p, Mnd2p and Apc9p Genetics, July 1, 2005; 170(3): 1045 - 1062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Kobirumaki, Y. Miyauchi, K. Fukami, and H. Tanaka A Novel UbcH10-Binding Protein Facilitates the Ubiquitinylation of Cyclin B In Vitro J. Biochem., February 1, 2005; 137(2): 133 - 139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Furukawa and Y. Xiong BTB Protein Keap1 Targets Antioxidant Transcription Factor Nrf2 for Ubiquitination by the Cullin 3-Roc1 Ligase Mol. Cell. Biol., January 1, 2005; 25(1): 162 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. G. Arnason, M. G. Pisclevich, M. D. Dash, G. F. Davies, and T. A. A. Harkness Novel Interaction between Apc5p and Rsp5p in an Intracellular Signaling Pathway in Saccharomyces cerevisiae Eukaryot. Cell, January 1, 2005; 4(1): 134 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, X. Mao, D. Ju, and Y. Xie Rpn4 Is a Physiological Substrate of the Ubr2 Ubiquitin Ligase J. Biol. Chem., December 31, 2004; 279(53): 55218 - 55223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Frazier, D. Shakes, U. Hota, and L. Boyd Caenorhabditis elegans UBC-2 functions with the anaphase-promoting complex but also has other activities J. Cell Sci., October 15, 2004; 117(22): 5427 - 5435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hatakeyama, M. Matsumoto, M. Yada, and K. I. Nakayama Interaction of U-box-type ubiquitin-protein ligases (E3s) with molecular chaperones Genes Cells, June 1, 2004; 9(6): 533 - 548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Koloteva-Levine, D. Pinchasi, I. Pereman, A. Zur, M. Brandeis, and O. Elroy-Stein The Apc5 Subunit of the Anaphase-Promoting Complex/Cyclosome Interacts with Poly(A) Binding Protein and Represses Internal Ribosome Entry Site-Mediated Translation Mol. Cell. Biol., May 1, 2004; 24(9): 3577 - 3587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schwickart, J. Havlis, B. Habermann, A. Bogdanova, A. Camasses, T. Oelschlaegel, A. Shevchenko, and W. Zachariae Swm1/Apc13 Is an Evolutionarily Conserved Subunit of the Anaphase-Promoting Complex Stabilizing the Association of Cdc16 and Cdc27 Mol. Cell. Biol., April 15, 2004; 24(8): 3562 - 3576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Konishi, J. Stegmuller, T. Matsuda, S. Bonni, and A. Bonni Cdh1-APC Controls Axonal Growth and Patterning in the Mammalian Brain Science, February 13, 2004; 303(5660): 1026 - 1030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Delhon, E. R. Tulman, C. L. Afonso, Z. Lu, A. de la Concha-Bermejillo, H. D. Lehmkuhl, M. E. Piccone, G. F. Kutish, and D. L. Rock Genomes of the Parapoxviruses Orf Virus and Bovine Papular Stomatitis Virus J. Virol., January 1, 2004; 78(1): 168 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Wirth, R. Ricci, J. F. Gimenez-Abian, S. Taghybeeglu, N. R. Kudo, W. Jochum, M. Vasseur-Cognet, and K. Nasmyth Loss of the anaphase-promoting complex in quiescent cells causes unscheduled hepatocyte proliferation Genes & Dev., January 1, 2004; 18(1): 88 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ramaswamy, J. S. Williams, K. M. Robinson, R. L. Sopko, and M. C. Schultz Global Control of Histone Modification by the Anaphase-Promoting Complex Mol. Cell. Biol., December 15, 2003; 23(24): 9136 - 9149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Deak, M. Donaldson, and D. M. Glover Mutations in makos, a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are suppressed by mutations in twins/aar, which encodes a regulatory subunit of PP2A J. Cell Sci., October 15, 2003; 116(20): 4147 - 4158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Michel, J. F. McCarville, and Y. Xiong A Role for Saccharomyces cerevisiae Cul8 Ubiquitin Ligase in Proper Anaphase Progression J. Biol. Chem., June 13, 2003; 278(25): 22828 - 22837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Seino, T. Kishi, H. Nishitani, and F. Yamao Two Ubiquitin-Conjugating Enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, Have Distinct Functions for Ubiquitination of Mitotic Cyclin Mol. Cell. Biol., May 15, 2003; 23(10): 3497 - 3505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Hall, M. P. Torres, G. K. Schroeder, and C. H. Borchers Mnd2 and Swm1 Are Core Subunits of the Saccharomyces cerevisiae Anaphase-promoting Complex J. Biol. Chem., May 2, 2003; 278(19): 16698 - 16705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xia, G. M. Pao, H.-W. Chen, I. M. Verma, and T. Hunter Enhancement of BRCA1 E3 Ubiquitin Ligase Activity through Direct Interaction with the BARD1 Protein J. Biol. Chem., February 7, 2003; 278(7): 5255 - 5263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Criqui, J. de Almeida Engler, A. Camasses, A. Capron, Y. Parmentier, D. Inze, and P. Genschik Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family Plant Physiology, November 1, 2002; 130(3): 1230 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. A. Harkness, G. F. Davies, V. Ramaswamy, and T. G. Arnason The Ubiquitin-Dependent Targeting Pathway in Saccharomyces cerevisiae Plays a Critical Role in Multiple Chromatin Assembly Regulatory Steps Genetics, October 1, 2002; 162(2): 615 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Harper, J. L. Burton, and M. J. Solomon The anaphase-promoting complex: it's not just for mitosis any more Genes & Dev., September 1, 2002; 16(17): 2179 - 2206. [Full Text] [PDF]
|
|
|
|
|
|
Y. Lin, W. C. Hwang, and R. Basavappa Structural and Functional Analysis of the Human Mitotic-specific Ubiquitin-conjugating Enzyme, UbcH10 J. Biol. Chem., June 7, 2002; 277(24): 21913 - 21921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Furukawa, T. Ohta, and Y. Xiong Activation of UBC5 Ubiquitin-conjugating Enzyme by the RING Finger of ROC1 and Assembly of Active Ubiquitin Ligases by All Cullins J. Biol. Chem., May 3, 2002; 277(18): 15758 - 15765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. Glickman and A. Ciechanover The Ubiquitin-Proteasome Proteolytic Pathway: Destruction for the Sake of Construction Physiol Rev, April 1, 2002; 82(2): 373 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chaturvedi, V. Sudakin, M. L. Bobiak, P. W. Fisher, M. R. Mattern, S. A. Jablonski, M. R. Hurle, Y. Zhu, T. J. Yen, and B.-B. S. Zhou Chfr Regulates a Mitotic Stress Pathway through its RING-Finger Domain with Ubiquitin Ligase Activity Cancer Res., March 1, 2002; 62(6): 1797 - 1801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Davis, L. Wille, B. A. Chestnut, P. L. Sadler, D. C. Shakes, and A. Golden Multiple Subunits of the Caenorhabditis elegans Anaphase-Promoting Complex Are Required for Chromosome Segregation During Meiosis I Genetics, February 1, 2002; 160(2): 805 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Yang, J. He, X. Lin, Q. Li, D. Pan, X. Zhang, and X. Xu Complete Genome Sequence of the Shrimp White Spot Bacilliform Virus J. Virol., December 1, 2001; 75(23): 11811 - 11820. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Tang, B. Li, R. Bharadwaj, H. Zhu, E. Ozkan, K. Hakala, J. Deisenhofer, and H. Yu APC2 Cullin Protein and APC11 RING Protein Comprise the Minimal Ubiquitin Ligase Module of the Anaphase-promoting Complex Mol. Biol. Cell, December 1, 2001; 12(12): 3839 - 3851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Stroschein, S. Bonni, J. L. Wrana, and K. Luo Smad3 recruits the anaphase-promoting complex for ubiquitination and degradation of SnoN Genes & Dev., November 1, 2001; 15(21): 2822 - 2836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Gardner, A. G. Shearer, and R. Y. Hampton In Vivo Action of the HRD Ubiquitin Ligase Complex: Mechanisms of Endoplasmic Reticulum Quality Control and Sterol Regulation Mol. Cell. Biol., July 1, 2001; 21(13): 4276 - 4291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang and Y. Xiong Control of p53 Ubiquitination and Nuclear Export by MDM2 and ARF Cell Growth Differ., April 1, 2001; 12(4): 175 - 186. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ohta and Y. Xiong Phosphorylation- and SKP1-independent in Vitro Ubiquitination of E2F1 by Multiple ROC-Cullin Ligases Cancer Res., February 1, 2001; 61(4): 1347 - 1353. [Abstract] [Full Text]
|
|
|
|
|
|
N Matsuda, T Suzuki, K Tanaka, and A Nakano Rma1, a novel type of RING finger protein conserved from Arabidopsis to human, is a membrane-bound ubiquitin ligase J. Cell Sci., January 5, 2001; 114(10): 1949 - 1957. [Abstract] [PDF]
|
|
|
|
|
|
H.-k. Huang, C. A. P. Joazeiro, E. Bonfoco, S. Kamada, J. D. Leverson, and T. Hunter The Inhibitor of Apoptosis, cIAP2, Functions as a Ubiquitin-Protein Ligase and Promotes in Vitro Monoubiquitination of Caspases 3 and 7 J. Biol. Chem., August 25, 2000; 275(35): 26661 - 26664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Ardley, N. G. S. Tan, S. A. Rose, A. F. Markham, and P. A. Robinson Features of the Parkin/Ariadne-like Ubiquitin Ligase, HHARI, That Regulate Its Interaction with the Ubiquitin-conjugating Enzyme, UbcH7 J. Biol. Chem., May 25, 2001; 276(22): 19640 - 19647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hatakeyama, M. Yada, M. Matsumoto, N. Ishida, and K.-I. Nakayama U Box Proteins as a New Family of Ubiquitin-Protein Ligases J. Biol. Chem., August 24, 2001; 276(35): 33111 - 33120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. May, N. Reynolds, C. F. Cullen, M. Yanagida, and H. Ohkura Polo boxes and Cut23 (Apc8) mediate an interaction between polo kinase and the anaphase-promoting complex for fission yeast mitosis J. Cell Biol., January 7, 2002; 156(1): 23 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kang, J. Chen, J. Wong, and G. Fang The checkpoint protein Chfr is a ligase that ubiquitinates Plk1 and inhibits Cdc2 at the G2 to M transition J. Cell Biol., January 21, 2002; 156(2): 249 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Leverson, H.-k. Huang, S. L. Forsburg, and T. Hunter The Schizosaccharomyces pombe Aurora-related Kinase Ark1 Interacts with the Inner Centromere Protein Pic1 and Mediates Chromosome Segregation and Cytokinesis Mol. Biol. Cell, April 1, 2002; 13(4): 1132 - 1143. [Abstract] [Full Text] [PDF]
|
|
