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Vol. 11, Issue 9, 3031-3044, September 2000

The Unique Catalytic Subunit of Sperm cAMP-dependent Protein Kinase Is the Product of an Alternative C mRNA Expressed Specifically in Spermatogenic Cells

Jovenal T. San Agustin, Curtis G. Wilkerson,^* and George B. Witman

Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655

cAMP-dependent protein kinase has a central role in the control of mammalian sperm capacitation and motility. Previous protein biochemical studies indicated that the only cAMP-dependent protein kinase catalytic subunit (C) in ovine sperm is an unusual isoform, termed C_s, whose amino terminus differs from those of published C isoforms of other species. Isolation and sequencing of cDNA clones encoding ovine C_s and C1 (the predominant somatic isoform) now reveal that C_s is the product of an alternative transcript of the C gene. C_s cDNA clones from murine and human testes also were isolated and sequenced, indicating that C_s is of ancient origin and widespread in mammals. In the mouse, C_s transcripts were detected only in testis and not in any other tissue examined, including ciliated tissues and ovaries. Finally, immunohistochemistry of the testis shows that C_s first appears in pachytene spermatocytes. This is the first demonstration of a cell type-specific expression for any C isoform. The conservation of C_s throughout mammalian evolution suggests that the unique structure of C_s is important in the subunit's localization or function within the sperm.

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^* Present address: Michigan State University-Department of Energy Plant Research Laboratory, East Lansing, MI 48824.

Corresponding author. E-mail address: george.witman{at}umassmed.edu.

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Molecular Biology of the Cell
Vol. 11, 3031-3044, September 2000
Copyright © 2000 by The American Society for Cell Biology

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