![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 9, 3045-3060, September 2000
and§
*Department of Cell Biology, Lerner Research Institute, and
§Urological Institute, The Cleveland Clinic Foundation,
Cleveland, Ohio 44195; In polarized Madin-Darby canine kidney epithelial cells, components
of the plasma membrane fusion machinery, the t-SNAREs syntaxin 2, 3, and 4 and SNAP-23, are differentially localized at the apical and/or
basolateral plasma membrane domains. Here we identify syntaxin 11 as a
novel apical and basolateral plasma membrane t-SNARE. Surprisingly, all
of these t-SNAREs redistribute to intracellular locations when
Madin-Darby canine kidney cells lose their cellular polarity. Apical
SNAREs relocalize to the previously characterized vacuolar apical
compartment, whereas basolateral SNAREs redistribute to a novel
organelle that appears to be the basolateral equivalent of the vacuolar
apical compartment. Both intracellular plasma membrane compartments
have an associated prominent actin cytoskeleton and receive membrane
traffic from cognate apical or basolateral pathways, respectively.
These findings demonstrate a fundamental shift in plasma membrane
traffic toward intracellular compartments while protein sorting is
preserved when epithelial cells lose their cell polarity.
Department of Anatomy, Department
of Biochemistry and Biophysics, Cardiovascular Research Institute,
University of California, San Francisco, California 94143; and
Experimental Immunology Branch, National Cancer
Institute, National Institutes of Health, Bethesda, Maryland 20892
Corresponding author. E-mail address:
weimbst{at}ccf.org.
This article has been cited by other articles:
|
|
 |
|
  A. Ferrari, A. Veligodskiy, U. Berge, M. S. Lucas, and R. Kroschewski ROCK-mediated contractility, tight junctions and channels contribute to the conversion of a preapical patch into apical surface during isochoric lumen initiation J. Cell Sci., November 1, 2008; 121(21): 3649 - 3663. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Dacks, P. P. Poon, and M. C. Field Phylogeny of endocytic components yields insight into the process of nonendosymbiotic organelle evolution PNAS, January 15, 2008; 105(2): 588 - 593. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Cohen, Y. Tian, and A. Musch Par1b Promotes Hepatic-type Lumen Polarity in Madin Darby Canine Kidney Cells via Myosin II- and E-Cadherin-dependent Signaling Mol. Biol. Cell, June 1, 2007; 18(6): 2203 - 2215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Low, A. Vasanji, J. Nanduri, M. He, N. Sharma, M. Koo, J. Drazba, and T. Weimbs Syntaxins 3 and 4 Are Concentrated in Separate Clusters on the Plasma Membrane before the Establishment of Cell Polarity Mol. Biol. Cell, February 1, 2006; 17(2): 977 - 989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wakabayashi, P. Dutt, J. Lippincott-Schwartz, and I. M. Arias Rab11a and myosin Vb are required for bile canalicular formation in WIF-B9 cells PNAS, October 18, 2005; 102(42): 15087 - 15092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Utech, A. I. Ivanov, S. N. Samarin, M. Bruewer, J. R. Turner, R. J. Mrsny, C. A. Parkos, and A. Nusrat Mechanism of IFN-{gamma}-induced Endocytosis of Tight Junction Proteins: Myosin II-dependent Vacuolarization of the Apical Plasma Membrane Mol. Biol. Cell, October 1, 2005; 16(10): 5040 - 5052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Tong, E. V. Vassilieva, A. I. Ivanov, Z. Wang, G. T. Brown, C. A. Parkos, and A. Nusrat Interferon-{gamma} Inhibits T84 Epithelial Cell Migration by Redirecting Transcytosis of {beta}1 Integrin from the Migrating Leading Edge J. Immunol., September 15, 2005; 175(6): 4030 - 4038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. I. Ivanov, D. Hunt, M. Utech, A. Nusrat, and C. A. Parkos Differential Roles for Actin Polymerization and a Myosin II Motor in Assembly of the Epithelial Apical Junctional Complex Mol. Biol. Cell, June 1, 2005; 16(6): 2636 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Shin, S. Straight, and B. Margolis PATJ regulates tight junction formation and polarity in mammalian epithelial cells J. Cell Biol., February 28, 2005; 168(5): 705 - 711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schuck and K. Simons Polarized sorting in epithelial cells: raft clustering and the biogenesis of the apical membrane J. Cell Sci., December 1, 2004; 117(25): 5955 - 5964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cohen, E. Rodriguez-Boulan, and A. Musch Par-1 promotes a hepatic mode of apical protein trafficking in MDCK cells PNAS, September 21, 2004; 101(38): 13792 - 13797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Morales-Tirado, S. Johannson, E. Hanson, A. Howell, J. Zhang, K. A. Siminovitch, and D. J. Fowell Cutting Edge: Selective Requirement for the Wiskott-Aldrich Syndrome Protein in Cytokine, but Not Chemokine, Secretion by CD4+ T Cells J. Immunol., July 15, 2004; 173(2): 726 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. I. Ivanov, A. Nusrat, and C. A. Parkos Endocytosis of Epithelial Apical Junctional Proteins by a Clathrin-mediated Pathway into a Unique Storage Compartment Mol. Biol. Cell, January 1, 2004; 15(1): 176 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kipp, S. Khoursandi, D. Scharlau, and R. K. H. Kinne More than apical: distribution of SGLT1 in Caco-2 cells Am J Physiol Cell Physiol, October 1, 2003; 285(4): C737 - C749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, S. H. Low, M. Miura, and T. Weimbs SNARE expression and localization in renal epithelial cells suggest mechanism for variability of trafficking phenotypes Am J Physiol Renal Physiol, November 1, 2002; 283(5): F1111 - F1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Tuma, L. K. Nyasae, and A. L. Hubbard Nonpolarized Cells Selectively Sort Apical Proteins from Cell Surface to a Novel Compartment, but Lack Apical Retention Mechanisms Mol. Biol. Cell, October 1, 2002; 13(10): 3400 - 3415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Dacks and W. F. Doolittle Novel syntaxin gene sequences from Giardia, Trypanosoma and algae: implications for the ancient evolution of the eukaryotic endomembrane system J. Cell Sci., April 15, 2002; 115(8): 1635 - 1642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Low, L. Y. Marmorstein, M. Miura, X. Li, N. Kudo, A. D. Marmorstein, and T. Weimbs Retinal pigment epithelial cells exhibit unique expression and localization of plasma membrane syntaxins which may contribute to their trafficking phenotype J. Cell Sci., January 12, 2002; 115(23): 4545 - 4553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zahraoui, D. Louvard, and T. Galli Tight Junction, a Platform for Trafficking and Signaling Protein Complexes J. Cell Biol., November 20, 2000; 151(5): F31 - F36. [Abstract] [Full Text] [PDF]
|
|
