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Vol. 11, Issue 9, 3045-3060, September 2000
and§
*Department of Cell Biology, Lerner Research Institute, and
§Urological Institute, The Cleveland Clinic Foundation,
Cleveland, Ohio 44195; In polarized Madin-Darby canine kidney epithelial cells, components
of the plasma membrane fusion machinery, the t-SNAREs syntaxin 2, 3, and 4 and SNAP-23, are differentially localized at the apical and/or
basolateral plasma membrane domains. Here we identify syntaxin 11 as a
novel apical and basolateral plasma membrane t-SNARE. Surprisingly, all
of these t-SNAREs redistribute to intracellular locations when
Madin-Darby canine kidney cells lose their cellular polarity. Apical
SNAREs relocalize to the previously characterized vacuolar apical
compartment, whereas basolateral SNAREs redistribute to a novel
organelle that appears to be the basolateral equivalent of the vacuolar
apical compartment. Both intracellular plasma membrane compartments
have an associated prominent actin cytoskeleton and receive membrane
traffic from cognate apical or basolateral pathways, respectively.
These findings demonstrate a fundamental shift in plasma membrane
traffic toward intracellular compartments while protein sorting is
preserved when epithelial cells lose their cell polarity.
Department of Anatomy, Department
of Biochemistry and Biophysics, Cardiovascular Research Institute,
University of California, San Francisco, California 94143; and
Experimental Immunology Branch, National Cancer
Institute, National Institutes of Health, Bethesda, Maryland 20892
Corresponding author. E-mail address:
weimbst{at}ccf.org.
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