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Vol. 11, Issue 9, 3123-3135, September 2000
§ and
*Department of Biology, The septins are a conserved family of proteins that are involved in
cytokinesis and other aspects of cell-surface organization. In
Drosophila melanogaster, null mutations in the
pnut septin gene are recessive lethal, but homozygous
pnut mutants complete embryogenesis and survive until
the pupal stage. Because the completion of cellularization and other
aspects of early development seemed likely to be due to maternally
contributed Pnut product, we attempted to generate embryos lacking the
maternal contribution in order to explore the roles of Pnut in these
processes. We used two methods, the production of germline clones
homozygous for a pnut mutation and the rescue of
pnut homozygous mutant flies by a
pnut+ transgene under control of the
hsp70 promoter. Remarkably, the pnut
germline-clone females produced eggs, indicating that stem-cell and
cystoblast divisions in the female germline do not require Pnut.
Moreover, the Pnut-deficient embryos obtained by either method
completed early syncytial development and began cellularization of the
embryo normally. However, during the later stages of cellularization, the organization of the actin cytoskeleton at the leading edge of the
invaginating furrows became progressively more abnormal, and the
embryos displayed widespread defects in cell and embryo morphology
beginning at gastrulation. Examination of two other septins showed that
Sep1 was not detectable at the cellularization front in the
Pnut-deficient embryos, whereas Sep2 was still present in normal
levels. Thus, it is possible that Sep2 (perhaps in conjunction with
other septins such as Sep4 and Sep5) fulfills an essential septin role
during the organization and initial ingression of the cellularization
furrow even in the absence of Pnut and Sep1. Together, the results
suggest that some cell-division events in Drosophila do
not require septin function, that there is functional differentiation
among the Drosophila septins, or both.
Program in Molecular Biology
and Biotechnology, and §Lineberger Comprehensive Cancer
Center, University of North Carolina, Chapel Hill, North Carolina 27599
Corresponding author: E-mail:
jpringle{at}emailunc.edu.
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