![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 9, 3219-3232, September 2000
and
Departments of *Cellular and Molecular Medicine and
Podocalyxin is a major membrane protein of the glomerular
epithelium and is thought to be involved in maintenance of the
architecture of the foot processes and filtration slits characteristic
of this unique epithelium by virtue of its high negative charge.
However, until now there has been no direct evidence for podocalyxin's function. Podocalyxin is a type 1 transmembrane sialoprotein with an
N-terminal mucin-like domain. To assess its function, we cloned rat
podocalyxin and examined the effects of its expression on the cell
adhesion properties of stably transfected Chinese hamster ovary
(CHO)-K1 and Madin-Darby canine kidney (MDCK) cells and inducible
ecdysone receptor-expressing (EcR)-CHO cells. In a cell aggregation
assay, CHO-K1 cells expressing high levels of podocalyxin showed
complete inhibition of cell aggregation, and MDCK transfectants showed
greatly reduced aggregation (~60-80%) compared with parental cells.
In EcR-CHO cells, the expression level of podocalyxin induced by
increasing levels of ecdysone analogue correlated closely with the
antiadhesion effect. The inhibitory effect of podocalyxin was reversed
by treatment of the cells with Arthrobacter ureafaciens sialidase, indicating that sialic acid is required for inhibition of
cell adhesion. Overexpression of podocalyxin also affected transepithelial resistance and the distribution of junctional proteins
in MDCK cells by an unknown mechanism that may involve interaction with
the actin cytoskeleton. These results provide direct evidence that
podocalyxin functions as an antiadhesin that maintains an open
filtration pathway between neighboring foot processes in the glomerular
epithelium by charge repulsion.
Pathology, University of California San Diego, La Jolla,
California 92093
Corresponding author. E-mail address:
mfarquhar{at}ucsd.edu.
This article has been cited by other articles:
|
|
 |
|
  K. Koop, M. Eikmans, M. Wehland, H. Baelde, D. Ijpelaar, R. Kreutz, H. Kawachi, D. Kerjaschki, E. de Heer, and J. A. Bruijn Selective Loss of Podoplanin Protein Expression Accompanies Proteinuria and Precedes Alterations in Podocyte Morphology in a Spontaneous Proteinuric Rat Model Am. J. Pathol., August 1, 2008; 173(2): 315 - 326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Choo, H. L. Tan, S. N. Ang, W. J. Fong, A. Chin, J. Lo, L. Zheng, H. Hentze, R. J. Philp, S. K.W. Oh, et al. Selection Against Undifferentiated Human Embryonic Stem Cells by a Cytotoxic Antibody Recognizing Podocalyxin-Like Protein-1 Stem Cells, June 1, 2008; 26(6): 1454 - 1463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sumiyoshi, J. Ricciuto, A. Tisdale, I. K. Gipson, F. Mantelli, and P. Argueso Antiadhesive Character of Mucin O-glycans at the Apical Surface of Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 197 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Nielsen and K. M. McNagny Influence of host irradiation on long-term engraftment by CD34-deficient hematopoietic stem cells Blood, August 1, 2007; 110(3): 1076 - 1077. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sizemore, M. Cicek, N. Sizemore, K. P. Ng, and G. Casey Podocalyxin Increases the Aggressive Phenotype of Breast and Prostate Cancer Cells In vitro through Its Interaction with Ezrin Cancer Res., July 1, 2007; 67(13): 6183 - 6191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M. Schopperle and W. C. DeWolf The TRA-1-60 and TRA-1-81 Human Pluripotent Stem Cell Markers Are Expressed on Podocalyxin in Embryonal Carcinoma Stem Cells, March 1, 2007; 25(3): 723 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Matsumoto, A. Shigeta, Y. Furukawa, T. Tanaka, M. Miyasaka, and T. Hirata CD43 Collaborates with P-Selectin Glycoprotein Ligand-1 to Mediate E-Selectin-Dependent T Cell Migration into Inflamed Skin J. Immunol., February 15, 2007; 178(4): 2499 - 2506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. S. Alexander, E. M. Viney, J.-G. Zhang, D. Metcalf, M. Kauppi, C. D. Hyland, M. R. Carpinelli, W. Stevenson, B. A. Croker, A. A. Hilton, et al. Thrombocytopenia and kidney disease in mice with a mutation in the C1galt1 gene PNAS, October 31, 2006; 103(44): 16442 - 16447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Tan, S. G.B. Furness, H. Merkens, S. Lin, M. L. McCoy, C. D. Roskelley, J. Kast, and K. M. McNagny Na+/H+ Exchanger Regulatory Factor-1 Is a Hematopoietic Ligand for a Subset of the CD34 Family of Stem Cell Surface Proteins Stem Cells, May 1, 2006; 24(5): 1150 - 1161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Casey, P. J. Neville, X. Liu, S. J. Plummer, M. S. Cicek, L. M. Krumroy, A. P. Curran, M. R. McGreevy, W. J. Catalona, E. A. Klein, et al. Podocalyxin variants and risk of prostate cancer and tumor aggressiveness Hum. Mol. Genet., March 1, 2006; 15(5): 735 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Harita, N. Miyauchi, T. Karasawa, K. Suzuki, G. D. Han, H. Koike, T. Igarashi, F. Shimizu, and H. Kawachi Altered expression of junctional adhesion molecule 4 in injured podocytes Am J Physiol Renal Physiol, February 1, 2006; 290(2): F335 - F344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Matsubara, A. Iwama, S. Yamazaki, C. Furuta, R. Hirasawa, Y. Morita, M. Osawa, T. Motohashi, K. Eto, H. Ema, et al. Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells throughout development J. Exp. Med., December 5, 2005; 202(11): 1483 - 1492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Cheng, Y.-Y. Lin, C.-Y. Yu, J.-Y. Chen, K.-F. Shen, W.-L. Lin, H.-K. Liao, Y.-J. Chen, C.-H. Liu, V. F. Pang, et al. Molecular Identification of Canine Podocalyxin-Like Protein 1 as a Renal Tubulogenic Regulator J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1612 - 1622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Doyonnas, J. S. Nielsen, S. Chelliah, E. Drew, T. Hara, A. Miyajima, and K. M. McNagny Podocalyxin is a CD34-related marker of murine hematopoietic stem cells and embryonic erythroid cells Blood, June 1, 2005; 105(11): 4170 - 4178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Meder, A. Shevchenko, K. Simons, and J. Fullekrug Gp135/podocalyxin and NHERF-2 participate in the formation of a preapical domain during polarization of MDCK cells J. Cell Biol., January 17, 2005; 168(2): 303 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Schmieder, M. Nagai, R. A. Orlando, T. Takeda, and M. G. Farquhar Podocalyxin Activates RhoA and Induces Actin Reorganization through NHERF1 and Ezrin in MDCK Cells J. Am. Soc. Nephrol., September 1, 2004; 15(9): 2289 - 2298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lehtonen, E. Lehtonen, K. Kudlicka, H. Holthofer, and M. G. Farquhar Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin Am. J. Pathol., September 1, 2004; 165(3): 923 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kanda, T. Tanaka, M. Matsumoto, E. Umemoto, Y. Ebisuno, M. Kinoshita, M. Noda, R. Kannagi, T. Hirata, T. Murai, et al. Endomucin, a sialomucin expressed in high endothelial venules, supports L-selectin-mediated rolling Int. Immunol., September 1, 2004; 16(9): 1265 - 1274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Stanhope-Baker, P. M. Kessler, W. Li, M. L. Agarwal, and B. R. G. Williams The Wilms Tumor Suppressor-1 Target Gene Podocalyxin Is Transcriptionally Repressed by p53 J. Biol. Chem., August 6, 2004; 279(32): 33575 - 33585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Somasiri, J. S. Nielsen, N. Makretsov, M. L. McCoy, L. Prentice, C. B. Gilks, S. K. Chia, K. A. Gelmon, D. B. Kershaw, D. G. Huntsman, et al. Overexpression of the Anti-Adhesin Podocalyxin Is an Independent Predictor of Breast Cancer Progression Cancer Res., August 1, 2004; 64(15): 5068 - 5073. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Economou, P. V. Kitsiou, A. K. Tzinia, E. Panagopoulou, E. Marinos, D. B. Kershaw, D. Kerjaschki, and E. C. Tsilibary Enhanced podocalyxin expression alters the structure of podocyte basal surface J. Cell Sci., July 1, 2004; 117(15): 3281 - 3294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tsuiji, S. Takasaki, M. Sakamoto, T. Irimura, and S. Hirohashi Aberrant O-glycosylation inhibits stable expression of dysadherin, a carcinoma-associated antigen, and facilitates cell-cell adhesion Glycobiology, July 1, 2003; 13(7): 521 - 527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Takeda, H. Yamazaki, and M. G. Farquhar Identification of an apical sorting determinant in the cytoplasmic tail of megalin Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1105 - C1113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Kreidberg Podocyte Differentiation and Glomerulogenesis J. Am. Soc. Nephrol., March 1, 2003; 14(3): 806 - 814. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Pavenstadt, W. Kriz, and M. Kretzler Cell Biology of the Glomerular Podocyte Physiol Rev, January 1, 2003; 83(1): 253 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Natoli, J. Liu, V. Eremina, K. Hodgens, C. Li, Y. Hamano, P. Mundel, R. Kalluri, J. H. Miner, S. E. Quaggin, et al. A Mutant Form of the Wilms' Tumor Suppressor Gene WT1 Observed in Denys-Drash Syndrome Interferes with Glomerular Capillary Development J. Am. Soc. Nephrol., August 1, 2002; 13(8): 2058 - 2067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rantanen, T. Palmen, A. Patari, H. Ahola, S. Lehtonen, E. Astrom, T. Floss, F. Vauti, W. Wurst, P. Ruiz, et al. Nephrin TRAP Mice Lack Slit Diaphragms and Show Fibrotic Glomeruli and Cystic Tubular Lesions J. Am. Soc. Nephrol., June 1, 2002; 13(6): 1586 - 1594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Simmons, R. J. Wool-Lewis, F. Baribaud, R. C. Netter, and P. Bates Ebola Virus Glycoproteins Induce Global Surface Protein Down-Modulation and Loss of Cell Adherence J. Virol., March 1, 2002; 76(5): 2518 - 2528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Park, S. G. Park, J.-W. Lee, T. Kim, G. Kim, Y.-G. Ko, and S. Kim Monocyte cell adhesion induced by a human aminoacyl-tRNA synthetase-associated factor, p43: identification of the related adhesion molecules and signal pathways J. Leukoc. Biol., February 1, 2002; 71(2): 223 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Dudek and J. G. N. Garcia Cytoskeletal regulation of pulmonary vascular permeability J Appl Physiol, October 1, 2001; 91(4): 1487 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. ORLANDO, T. TAKEDA, B. ZAK, S. SCHMIEDER, V. M. BENOIT, T. MCQUISTAN, H. FURTHMAYR, and M. G. FARQUHAR The Glomerular Epithelial Cell Anti-Adhesin Podocalyxin Associates with the Actin Cytoskeleton through Interactions with Ezrin J. Am. Soc. Nephrol., August 1, 2001; 12(8): 1589 - 1598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Doyonnas, D. B. Kershaw, C. Duhme, H. Merkens, S. Chelliah, T. Graf, and K. M. McNagny Anuria, Omphalocele, and Perinatal Lethality in Mice Lacking the CD34-related Protein Podocalyxin J. Exp. Med., June 25, 2001; 194(1): 13 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, J. Li, S. W. Straight, and D. B. Kershaw PDZ domain-mediated interaction of rabbit podocalyxin and Na+/H+ exchange regulatory factor-2 Am J Physiol Renal Physiol, June 1, 2002; 282(6): F1129 - F1139. [Abstract] [Full Text] [PDF]
|
|
