![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 11, Issue 9, 3247-3263, September 2000
and
*Departments of Cell Biology & Anatomy, The carboxy-terminal 150 residues of the focal adhesion kinase
(FAK) comprise the focal adhesion-targeting sequence, which is
responsible for its subcellular localization. The mechanism of focal
adhesion targeting has not been fully elucidated. We describe a
mutational analysis of the focal adhesion-targeting sequence of FAK to
further examine the mechanism of focal adhesion targeting and explore
additional functions encoded by the carboxy-terminus of FAK. The
results demonstrate that paxillin binding is dispensable for focal
adhesion targeting of FAK. Cell adhesion-dependent tyrosine phosphorylation strictly correlated with the ability of mutants to
target to focal adhesions. Focal adhesion targeting was also a
requirement for maximal FAK-dependent tyrosine phosphorylation of
paxillin and FAK-related nonkinase (FRNK)-dependent inhibition of
endogenous FAK function. However, there were additional requirements for these latter functions because we identified mutants that target to
focal adhesions, yet are defective for the induction of paxillin
phosphorylation or the dominant-negative function of FRNK. Furthermore,
the paxillin-binding activity of FRNK mutants did not correlate with
their ability to inhibit FAK, suggesting that FRNK has other targets in
addition to paxillin.
Pediatrics,
and Anesthesiology, and §Lineberger
Comprehensive Cancer Center, University of North Carolina, Chapel Hill,
North Carolina 27599
Corresponding author: E-mail address:
crispy4{at}med.unc.edu.
This article has been cited by other articles:
|
|
 |
|
  F. Chang, C. A. Lemmon, D. Park, and L. H. Romer FAK Potentiates Rac1 Activation and Localization to Matrix Adhesion Sites: A Role for betaPIX Mol. Biol. Cell, January 1, 2007; 18(1): 253 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Khare, C. Holgren, and A. M. Samarel Deoxycholic acid differentially regulates focal adhesion kinase phosphorylation: role of tyrosine phosphatase ShP2 Am J Physiol Gastrointest Liver Physiol, December 1, 2006; 291(6): G1100 - G1112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Cai, M. Li, J. Vrana, and M. D. Schaller Glycogen Synthase Kinase 3- and Extracellular Signal-Regulated Kinase-Dependent Phosphorylation of Paxillin Regulates Cytoskeletal Rearrangement Mol. Cell. Biol., April 1, 2006; 26(7): 2857 - 2868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Cohen and J.-L. Guan Residues within the First Subdomain of the FERM-like Domain in Focal Adhesion Kinase Are Important in Its Regulation J. Biol. Chem., March 4, 2005; 280(9): 8197 - 8207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. A. K. M. Khyrul, D. P. LaLonde, M. C. Brown, H. Levinson, and C. E. Turner The Integrin-linked Kinase Regulates Cell Morphology and Motility in a Rho-associated Kinase-dependent Manner J. Biol. Chem., December 24, 2004; 279(52): 54131 - 54139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Giannone, P. Ronde, M. Gaire, J. Beaudouin, J. Haiech, J. Ellenberg, and K. Takeda Calcium Rises Locally Trigger Focal Adhesion Disassembly and Enhance Residency of Focal Adhesion Kinase at Focal Adhesions J. Biol. Chem., July 2, 2004; 279(27): 28715 - 28723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Dunty, V. Gabarra-Niecko, M. L. King, D. F. J. Ceccarelli, M. J. Eck, and M. D. Schaller FERM Domain Interaction Promotes FAK Signaling Mol. Cell. Biol., June 15, 2004; 24(12): 5353 - 5368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gao, K. C. Prutzman, M. L. King, D. M. Scheswohl, E. F. DeRose, R. E. London, M. D. Schaller, and S. L. Campbell NMR Solution Structure of the Focal Adhesion Targeting Domain of Focal Adhesion Kinase in Complex with a Paxillin LD Peptide: EVIDENCE FOR A TWO-SITE BINDING MODEL J. Biol. Chem., February 27, 2004; 279(9): 8441 - 8451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Cooper, T.-L. Shen, and J.-L. Guan Regulation of Focal Adhesion Kinase by Its Amino-Terminal Domain through an Autoinhibitory Interaction Mol. Cell. Biol., November 15, 2003; 23(22): 8030 - 8041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kwong, M. A. Wozniak, A. S. Collins, S. D. Wilson, and P. J. Keely R-Ras Promotes Focal Adhesion Formation through Focal Adhesion Kinase and p130Cas by a Novel Mechanism That Differs from Integrins Mol. Cell. Biol., February 1, 2003; 23(3): 933 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Toutant, A. Costa, J.-M. Studler, G. Kadare, M. Carnaud, and J.-A. Girault Alternative Splicing Controls the Mechanisms of FAK Autophosphorylation Mol. Cell. Biol., November 15, 2002; 22(22): 7731 - 7743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Dunty and M. D. Schaller The N Termini of Focal Adhesion Kinase Family Members Regulate Substrate Phosphorylation, Localization, and Cell Morphology J. Biol. Chem., November 15, 2002; 277(47): 45644 - 45654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Liu, C. D. Guibao, and J. Zheng Structural Insight into the Mechanisms of Targeting and Signaling of Focal Adhesion Kinase Mol. Cell. Biol., April 15, 2002; 22(8): 2751 - 2760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. D. Lyons, J. M. Dunty, E. M. Schaefer, and M. D. Schaller Inhibition of the Catalytic Activity of Cell Adhesion Kinase beta by Protein-tyrosine Phosphatase-PEST-mediated Dephosphorylation J. Biol. Chem., June 22, 2001; 276(26): 24422 - 24431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wu, G. E. Davis, G. A. Meininger, E. Wilson, and M. J. Davis Regulation of the L-type Calcium Channel by alpha 5beta 1 Integrin Requires Signaling between Focal Adhesion Proteins J. Biol. Chem., August 3, 2001; 276(32): 30285 - 30292. [Abstract] [Full Text] [PDF]
|
|
