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Vol. 12, Issue 1, 171-184, January 2001

Competition for Microtubule-binding with Dual Expression of Tau Missense and Splice Isoforms

Mei Lu, and Kenneth S. Kosik

Center for Neurologic Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115

How tau mutations lead to neurodegeneration is unknown but may be related to altered microtubule binding properties of mutant tau protein. The tendency for the mutations to cluster around the microtubule-binding domain of tau or to alter the ratios of those splice isoforms that affect binding supports the view that the tau/microtubule interaction is critical and finely regulated. In cells transfected with both mutant and wild-type tau isoforms fused to either yellow fluorescent protein or cyan fluorescent protein we can observe tau fusion proteins that differ by a single amino acid or by the inclusion or exclusion of exon 10. With coexpression of mutant and wild-type tau, the mutant isoform appears diffuse throughout the cytoplasm; however, when mutant tau is expressed alone, it appears mostly bound to the microtubules. Dual imaging of the three- and four-repeat tau isoforms indicated that the expression of four-repeat tau displaced three-repeat tau from the microtubules. These results suggest that altered kinetic competition among the isoforms for microtubule binding could be a disease precipitant.

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Corresponding author. E-mail address: kosik{at}cnd.bwh.harvard.edu.

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Molecular Biology of the Cell
Vol. 12, 171-184, January 2001
Copyright © 2001 by The American Society for Cell Biology

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