Structure and Function of a Vimentin-associated Matrix Adhesion in Endothelial Cells

QUICK SEARCH:

[advanced]

	Author:		Keyword(s):
Year:		Vol:		Page:

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager


Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Gonzales, M.
	Articles by Jones, J. C. R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Gonzales, M.
	Articles by Jones, J. C. R.

Vol. 12, Issue 1, 85-100, January 2001

Structure and Function of a Vimentin-associated Matrix Adhesion in Endothelial Cells

Meredith Gonzales,^* Babette Weksler, Daisuke Tsuruta,^* Robert D. Goldman,^* Kristine J. Yoon,^* Susan B. Hopkinson,^* Frederick W. Flitney, and Jonathan C. R. Jones^*^§

^*Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611; Department of Medicine, Weill Medical College of Cornell University, New York, New York 10021; and Centre for Biomedical Sciences, School of Biology, University of St. Andrews, St. Andrews, Scotland KY16 9TS

The $alpha$ 4 laminin subunit is a component of endothelial cell basement membranes. An antibody (2A3) against the $alpha$ 4 laminin G domainstains focal contact-like structures in transformed and primarymicrovascular endothelial cells (TrHBMECs and HMVECs, respectively),provided the latter cells are activated with growth factors. The2A3 antibody staining colocalizes with that generated by $alpha$ v and $beta$ 3 integrin antibodies and, consistent with this localization,TrHBMECs and HMVECs adhere to the $alpha$ 4 laminin subunit G domainin an $alpha$ v $beta$ 3-integrin-dependent manner. The $alpha$ v $beta$ 3 integrin/2A3 antibodypositively stained focal contacts are recognized by vinculin antibodiesas well as by antibodies against plectin. Unusually, vimentinintermediate filaments, in addition to microfilament bundles,interact with many of the $alpha$ v $beta$ 3 integrin-positive focal contacts.We have investigated the function of $alpha$ 4-laminin and $alpha$ v $beta$ 3-integrin,which are at the core of these focal contacts, in cultured endothelialcells. Antibodies against these proteins inhibit branching morphogenesisof TrHBMECs and HMVECs in vitro, as well as their ability to repopulatein vitro wounds. Thus, we have characterized an endothelial cellmatrix adhesion, which shows complex cytoskeletal interactionsand whose assembly is regulated by growth factors. Our data indicatethat this adhesion structure may play a role inangiogenesis.

^§ Corresponding author. E-mail address: j-jones3{at}nwu.edu.

This article has been cited by other articles:

T. R. Carlson, H. Hu, R. Braren, Y. H. Kim, and R. A. Wang
Cell-autonomous requirement for {beta}1 integrin in endothelial cell adhesion, migration and survival during angiogenesis in mice
Development, June 15, 2008; 135(12): 2193 - 2202.
[Abstract] [Full Text] [PDF]

M. O'Rourke, C. Ward, J. Worthington, J. McKenna, A. Valentine, T. Robson, D. G. Hirst, and S. R. McKeown
Evaluation of the Antiangiogenic Potential of AQ4N
Clin. Cancer Res., March 1, 2008; 14(5): 1502 - 1509.
[Abstract] [Full Text] [PDF]

A. K. Berfield, K. M. Hansen, and C. K. Abrass
Rat glomerular mesangial cells require laminin-9 to migrate in response to insulin-like growth factor binding protein-5
Am J Physiol Cell Physiol, October 1, 2006; 291(4): C589 - C599.
[Abstract] [Full Text] [PDF]

J. R. van Beijnum, R. P. Dings, E. van der Linden, B. M. M. Zwaans, F. C. S. Ramaekers, K. H. Mayo, and A. W. Griffioen
Gene expression of tumor angiogenesis dissected: specific targeting of colon cancer angiogenic vasculature
Blood, October 1, 2006; 108(7): 2339 - 2348.
[Abstract] [Full Text] [PDF]

N Uyama, L Zhao, E Van Rossen, Y Hirako, H Reynaert, D H Adams, Z Xue, Z Li, R Robson, M Pekny, et al.
Hepatic stellate cells express synemin, a protein bridging intermediate filaments to focal adhesions
Gut, September 1, 2006; 55(9): 1276 - 1289.
[Abstract] [Full Text] [PDF]

R. Hallmann, N. Horn, M. Selg, O. Wendler, F. Pausch, and L. M. Sorokin
Expression and Function of Laminins in the Embryonic and Mature Vasculature
Physiol Rev, July 1, 2005; 85(3): 979 - 1000.
[Abstract] [Full Text] [PDF]

D. Tsuruta and J. C. R. Jones
The vimentin cytoskeleton regulates focal contact size and adhesion of endothelial cells subjected to shear stress
J. Cell Sci., December 15, 2003; 116(24): 4977 - 4984.
[Abstract] [Full Text] [PDF]

C. Gilles, M. Polette, M. Mestdagt, B. Nawrocki-Raby, P. Ruggeri, P. Birembaut, and J.-M. Foidart
Transactivation of Vimentin by {beta}-Catenin in Human Breast Cancer Cells
Cancer Res., May 15, 2003; 63(10): 2658 - 2664.
[Abstract] [Full Text] [PDF]

A. M. Gonzalez, M. Gonzales, G. S. Herron, U. Nagavarapu, S. B. Hopkinson, D. Tsuruta, and J. C. R. Jones
Complex interactions between the laminin alpha 4 subunit and integrins regulate endothelial cell behavior in vitro and angiogenesis in vivo
PNAS, December 10, 2002; 99(25): 16075 - 16080.
[Abstract] [Full Text] [PDF]

A. P. J. van den Heuvel, A. M. M. de Vries-Smits, P. C. van Weeren, P. F. Dijkers, K. M. T. de Bruyn, J. A. Riedl, and B. M. T. Burgering
Binding of protein kinase B to the plakin family member periplakin
J. Cell Sci., October 15, 2002; 115(20): 3957 - 3966.
[Abstract] [Full Text] [PDF]

				M. O'Rourke, C. Ward, J. Worthington, J. McKenna, A. Valentine, T. Robson, D. G. Hirst, and S. R. McKeown Evaluation of the Antiangiogenic Potential of AQ4N Clin. Cancer Res., March 1, 2008; 14(5): 1502 - 1509. [Abstract] [Full Text] [PDF]

				A. K. Berfield, K. M. Hansen, and C. K. Abrass Rat glomerular mesangial cells require laminin-9 to migrate in response to insulin-like growth factor binding protein-5 Am J Physiol Cell Physiol, October 1, 2006; 291(4): C589 - C599. [Abstract] [Full Text] [PDF]

				J. R. van Beijnum, R. P. Dings, E. van der Linden, B. M. M. Zwaans, F. C. S. Ramaekers, K. H. Mayo, and A. W. Griffioen Gene expression of tumor angiogenesis dissected: specific targeting of colon cancer angiogenic vasculature Blood, October 1, 2006; 108(7): 2339 - 2348. [Abstract] [Full Text] [PDF]

				N Uyama, L Zhao, E Van Rossen, Y Hirako, H Reynaert, D H Adams, Z Xue, Z Li, R Robson, M Pekny, et al. Hepatic stellate cells express synemin, a protein bridging intermediate filaments to focal adhesions Gut, September 1, 2006; 55(9): 1276 - 1289. [Abstract] [Full Text] [PDF]

				R. Hallmann, N. Horn, M. Selg, O. Wendler, F. Pausch, and L. M. Sorokin Expression and Function of Laminins in the Embryonic and Mature Vasculature Physiol Rev, July 1, 2005; 85(3): 979 - 1000. [Abstract] [Full Text] [PDF]

				D. Tsuruta and J. C. R. Jones The vimentin cytoskeleton regulates focal contact size and adhesion of endothelial cells subjected to shear stress J. Cell Sci., December 15, 2003; 116(24): 4977 - 4984. [Abstract] [Full Text] [PDF]

				C. Gilles, M. Polette, M. Mestdagt, B. Nawrocki-Raby, P. Ruggeri, P. Birembaut, and J.-M. Foidart Transactivation of Vimentin by {beta}-Catenin in Human Breast Cancer Cells Cancer Res., May 15, 2003; 63(10): 2658 - 2664. [Abstract] [Full Text] [PDF]

				A. M. Gonzalez, M. Gonzales, G. S. Herron, U. Nagavarapu, S. B. Hopkinson, D. Tsuruta, and J. C. R. Jones Complex interactions between the laminin alpha 4 subunit and integrins regulate endothelial cell behavior in vitro and angiogenesis in vivo PNAS, December 10, 2002; 99(25): 16075 - 16080. [Abstract] [Full Text] [PDF]

				A. P. J. van den Heuvel, A. M. M. de Vries-Smits, P. C. van Weeren, P. F. Dijkers, K. M. T. de Bruyn, J. A. Riedl, and B. M. T. Burgering Binding of protein kinase B to the plakin family member periplakin J. Cell Sci., October 15, 2002; 115(20): 3957 - 3966. [Abstract] [Full Text] [PDF]