![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 10, 3087-3094, October 2001
Departments of Surgery and Pathology, Children's Hospital and
Harvard Medical School, Boston, Massachusetts 02115.
Capillary endothelial cells can be switched between growth and
apoptosis by modulating their shape with the use of micropatterned adhesive islands. The present study was carried out to examine whether cytoskeletal filaments contribute to this response. Disruption of microfilaments or microtubules with the use of cytochalasin D or
nocodazole, respectively, led to levels of apoptosis in capillary cells
equivalent to that previously demonstrated by inducing cell rounding
with the use of micropatterned culture surfaces containing small (<20
µm in diameter) circular adhesive islands coated with fibronectin.
Simultaneous disruption of microfilaments and microtubules led to more
pronounced cell rounding and to enhanced levels of apoptosis
approaching that observed during anoikis in fully detached (suspended)
cells, indicating that these two cytoskeletal filament systems can
cooperate to promote cell survival. Western blot analysis revealed that
the protein kinase Akt, which is known to be critical for control of
cell survival became dephosphorylated during cell rounding induced by
disruption of the cytoskeleton, and that this was accompanied by a
decrease in bcl-2 expression as well as a subsequent increase in
caspase activation. This ability of the cytoskeleton to control
capillary endothelial cell survival may be important for understanding
the relationship among extracellular matrix turnover, cell shape
changes, and apoptosis during angiogenesis inhibition.
This article has been cited by other articles:
|
|
 |
|
  A. Della Corte, C. Quarto, C. Bancone, C. Castaldo, F. Di Meglio, D. Nurzynska, L. S. De Santo, M. De Feo, M. Scardone, S. Montagnani, et al. Spatiotemporal patterns of smooth muscle cell changes in ascending aortic dilatation with bicuspid and tricuspid aortic valve stenosis: Focus on cell matrix signaling J. Thorac. Cardiovasc. Surg., January 1, 2008; 135(1): 8 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ostergaard, E. Stankevicius, M. R. Andersen, Y. Eskildsen-Helmond, T. Ledet, M. J. Mulvany, and U. Simonsen Diminished NO release in chronic hypoxic human endothelial cells Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2894 - H2903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Aarabi, K. A. Bhatt, Y. Shi, J. Paterno, E. I. Chang, S. A. Loh, J. W. Holmes, M. T. Longaker, H. Yee, and G. C. Gurtner Mechanical load initiates hypertrophic scar formation through decreased cellular apoptosis FASEB J, October 1, 2007; 21(12): 3250 - 3261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Deschesnes, A. Patenaude, J. L. C. Rousseau, J. S. Fortin, C. Ricard, M.-F. Cote, J. Huot, R. C.-Gaudreault, and E. Petitclerc Microtubule-Destabilizing Agents Induce Focal Adhesion Structure Disorganization and Anoikis in Cancer Cells J. Pharmacol. Exp. Ther., February 1, 2007; 320(2): 853 - 864. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Triplett and F. M. Pavalko Disruption of {alpha}-actinin-integrin interactions at focal adhesions renders osteoblasts susceptible to apoptosis Am J Physiol Cell Physiol, November 1, 2006; 291(5): C909 - C921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. F. Weber and A. S. Menko Phosphatidylinositol 3-kinase is necessary for lens fiber cell differentiation and survival. Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4490 - 4499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yokouchi, Y. Numaguchi, R. Kubota, M. Ishii, H. Imai, R. Murakami, Y. Ogawa, T. Kondo, K. Okumura, D. E. Ingber, et al. l-Caldesmon Regulates Proliferation and Migration of Vascular Smooth Muscle Cells and Inhibits Neointimal Formation After Angioplasty Arterioscler. Thromb. Vasc. Biol., October 1, 2006; 26(10): 2231 - 2237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lypowy, I.-Y. Chen, and M. Abdellatif An Alliance between Ras GTPase-activating Protein, Filamin C, and Ras GTPase-activating Protein SH3 Domain-binding Protein Regulates Myocyte Growth J. Biol. Chem., July 8, 2005; 280(27): 25717 - 25728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Pinkas, S. S. Martin, and P. Leder Bcl-2-Mediated Cell Survival Promotes Metastasis of EpH4 {beta}MEKDD Mammary Epithelial Cells Mol. Cancer Res., October 1, 2004; 2(10): 551 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Li, T. Zhu, and K.-L. Guan Transformation Potential of Ras Isoforms Correlates with Activation of Phosphatidylinositol 3-Kinase but Not ERK J. Biol. Chem., September 3, 2004; 279(36): 37398 - 37406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Bayless and G. E. Davis Microtubule Depolymerization Rapidly Collapses Capillary Tube Networks in Vitro and Angiogenic Vessels in Vivo through the Small GTPase Rho J. Biol. Chem., March 19, 2004; 279(12): 11686 - 11695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-J. Kim, S.-G. Hwang, I.-C. Kim, and J.-S. Chun Actin Cytoskeletal Architecture Regulates Nitric Oxide-induced Apoptosis, Dedifferentiation, and Cyclooxygenase-2 Expression in Articular Chondrocytes via Mitogen-activated Protein Kinase and Protein Kinase C Pathways J. Biol. Chem., October 24, 2003; 278(43): 42448 - 42456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Ingber Tensegrity II. How structural networks influence cellular information processing networks J. Cell Sci., April 15, 2003; 116(8): 1397 - 1408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Ingber Mechanosensation through integrins: Cells act locally but think globally PNAS, February 18, 2003; 100(4): 1472 - 1474. [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Ingber Mechanical Signaling and the Cellular Response to Extracellular Matrix in Angiogenesis and Cardiovascular Physiology Circ. Res., November 15, 2002; 91(10): 877 - 887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tran, Z. Master, J. L. Yu, J. Rak, D. J. Dumont, and R. S. Kerbel A role for survivin in chemoresistance of endothelial cells mediated by VEGF PNAS, April 2, 2002; 99(7): 4349 - 4354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Stupack and D. A. Cheresh Get a ligand, get a life: integrins, signaling and cell survival J. Cell Sci., January 10, 2002; 115(19): 3729 - 3738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tran, Z. Master, J. L. Yu, J. Rak, D. J. Dumont, and R. S. Kerbel A role for survivin in chemoresistance of endothelial cells mediated by VEGF PNAS, April 2, 2002; 99(7): 4349 - 4354. [Abstract] [Full Text] [PDF]
|
|
