Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reddy, J. V.
Right arrow Articles by Seaman, M. N.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reddy, J. V.
Right arrow Articles by Seaman, M. N.J.

Vol. 12, Issue 10, 3242-3256, October 2001

Vps26p, a Component of Retromer, Directs the Interactions of Vps35p in Endosome-to-Golgi Retrieval

Jonathan V. Reddy, and Matthew N.J. Seaman*

Department Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, Addenbrookes Hospital, Cambridge, CB2 2XY, United Kingdom

Endosome-to-Golgi retrieval of the carboxypeptidase Y receptor Vps10p is mediated by a recently discovered membrane coat complex termed retromer. Retromer comprises five conserved proteins: Vps35p, Vps29p, Vps5p, Vps17p, and Vps26p. Vps35p recognizes cargo molecules such as Vps10p and interacts strongly with Vps29p. Vps5p forms a subcomplex with Vps17p and has been proposed to play a structural role by self-assembling into large multimeric structures. The function of Vps26p is currently unknown. We have investigated the role that Vps26p plays in retromer-mediated endosome-to-Golgi transport by analyzing dominant negative alleles of Vps26p. These mutants have identified a crucial region of Vps26p that plays an important role in its function. Functional domains of Vps26p have been investigated by the creation of yeast-mouse hybrid molecules in which domains of Vps26p have been replaced by the similar domain in the protein encoded by the mouse VPS26 gene, Hbeta 58. These domain swap experiments have shown that Vps26p promotes the interactions between the cargo-selective component Vps35p and the structural components Vps5p/Vps17p.

* Corresponding author. E-mail address: mnjs100{at}cam.ac.uk.

Molecular Biology of the Cell
Vol. 12, 3242-3256, October 2001
Copyright © 2001 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 Mol PlantHome page
N. Q. Phan, S.-J. Kim, and D. C. Bassham
Overexpression of Arabidopsis Sorting Nexin AtSNX2b Inhibits Endocytic Trafficking to the Vacuole
Mol Plant, October 3, 2008; (2008) ssn057v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. G. Robinson, L. Jiang, and K. Schumacher
The Endosomal System of Plants: Charting New and Familiar Territories
Plant Physiology, August 1, 2008; 147(4): 1482 - 1492.
[Full Text] [PDF]

Home page
 ScienceHome page
K. Tarassov, V. Messier, C. R. Landry, S. Radinovic, M. M. S. Molina, I. Shames, Y. Malitskaya, J. Vogel, H. Bussey, and S. W. Michnick
An in Vivo Map of the Yeast Protein Interactome
Science, June 13, 2008; 320(5882): 1465 - 1470.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. Nakano, T. Yamamoto, T. Kishimoto, T. Noji, and K. Tanaka
Protein Kinases Fpk1p and Fpk2p are Novel Regulators of Phospholipid Asymmetry
Mol. Biol. Cell, April 1, 2008; 19(4): 1783 - 1797.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. E. Techau, J. Valdez-Taubas, J.-F. Popoff, R. Francis, M. Seaman, and J. M. Blackwell
Evolution of Differences in Transport Function in Slc11a Family Members
J. Biol. Chem., December 7, 2007; 282(49): 35646 - 35656.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. N. J. Seaman
Identification of a novel conserved sorting motif required for retromer-mediated endosome-to-TGN retrieval
J. Cell Sci., July 15, 2007; 120(14): 2378 - 2389.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. V. Bujny, V. Popoff, L. Johannes, and P. J. Cullen
The retromer component sorting nexin-1 is required for efficient retrograde transport of Shiga toxin from early endosome to the trans Golgi network
J. Cell Sci., June 15, 2007; 120(12): 2010 - 2021.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Rubio-Texeira and C. A. Kaiser
Amino Acids Regulate Retrieval of the Yeast General Amino Acid Permease from the Vacuolar Targeting Pathway
Mol. Biol. Cell, July 1, 2006; 17(7): 3031 - 3050.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
T. Iwaki, A. Hosomi, S. Tokudomi, Y. Kusunoki, Y. Fujita, Y. Giga-Hama, N. Tanaka, and K. Takegawa
Vacuolar protein sorting receptor in Schizosaccharomyces pombe.
Microbiology, May 1, 2006; 152(Pt 5): 1523 - 1532.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
B. C. Prasad and S. G. Clark
Wnt signaling establishes anteroposterior neuronal polarity and requires retromer in C. elegans
Development, May 1, 2006; 133(9): 1757 - 1766.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Oliviusson, O. Heinzerling, S. Hillmer, G. Hinz, Y. C. Tse, L. Jiang, and D. G. Robinson
Plant Retromer, Localized to the Prevacuolar Compartment and Microvesicles in Arabidopsis, May Interact with Vacuolar Sorting Receptors
PLANT CELL, May 1, 2006; 18(5): 1239 - 1252.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. Nakada-Tsukui, Y. Saito-Nakano, V. Ali, and T. Nozaki
A Retromerlike Complex Is a Novel Rab7 Effector That Is Involved in the Transport of the Virulence Factor Cysteine Protease in the Enteric Protozoan Parasite Entamoeba histolytica
Mol. Biol. Cell, November 1, 2005; 16(11): 5294 - 5303.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Wang, M. Guo, Z. Liang, J. Fan, Z. Zhu, J. Zang, Z. Zhu, X. Li, M. Teng, L. Niu, et al.
Crystal Structure of Human Vacuolar Protein Sorting Protein 29 Reveals a Phosphodiesterase/Nuclease-like Fold and Two Protein-Protein Interaction Sites
J. Biol. Chem., June 17, 2005; 280(24): 22962 - 22967.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. Nazarian, K. Bouri, and E. P. Hoffman
Intracellular expression profiling by laser capture microdissection: three novel components of the neuromuscular junction
Physiol Genomics, March 21, 2005; 21(1): 70 - 80.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. N.J. Seaman
Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer
J. Cell Biol., April 12, 2004; 165(1): 111 - 122.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
C. N. Arighi, L. M. Hartnell, R. C. Aguilar, C. R. Haft, and J. S. Bonifacino
Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor
J. Cell Biol., April 12, 2004; 165(1): 123 - 133.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Burda, S. M. Padilla, S. Sarkar, and S. D. Emr
Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase
J. Cell Sci., October 15, 2002; 115(20): 3889 - 3900.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. G. Schwarz, C. T. Griffin, E. A. Schneider, D. Yee, and T. Magnuson
Genetic Analysis of Sorting Nexins 1 and 2 Reveals a Redundant and Essential Function in Mice
Mol. Biol. Cell, October 1, 2002; 13(10): 3588 - 3600.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. N.J. Seaman and H. P. Williams
Identification of the Functional Domains of Yeast Sorting Nexins Vps5p and Vps17p
Mol. Biol. Cell, August 1, 2002; 13(8): 2826 - 2840.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]