![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 11, 3340-3352, November 2001
-Tubulin Complex
§ and
*Departments of Biological Sciences and Genetics, Stanford
University, Stanford, California 94305-5020; The Genentech,
Inc., South San Francisco, California 94080; and
Department of Biochemistry and Biophysics,
§Howard Hughes Medical Institute, University of
California, San Francisco, California 94143
-tubulin complex is a large multiprotein complex that is
required for microtubule nucleation at the centrosome. Here we report
the purification and characterization of the human -tubulin complex
and the identification of its subunits. The human -tubulin complex
is a ring of ~25 nm, has a subunit structure similar to that reported
for -tubulin complexes from other species, and is able to nucleate
microtubule polymerization in vitro. Mass spectrometry analysis of the
human -tubulin complex components confirmed the presence of four
previously identified components (-tubulin and -tubulin complex
proteins [GCPs] 2, 3, and 4) and led to the identification of two new
components, GCP5 and GCP6. Sequence analysis revealed that the GCPs
share five regions of sequence similarity and define a novel protein
superfamily that is conserved in metazoans. GCP5 and GCP6, like other
components of the -tubulin complex, localize to the centrosome and
associate with microtubules, suggesting that the entire -tubulin
complex takes part in both of these interactions. Stoichiometry
experiments revealed that there is a single copy of GCP5 and multiple
copies of -tubulin, GCP2, GCP3, and GCP4 within the -tubulin
complex. Thus, the -tubulin complex is conserved in structure and
function, suggesting that the mechanism of microtubule nucleation is conserved.
Corresponding author. E-mail address:
stearns{at}stanford.edu.
This article has been cited by other articles:
|
|
 |
|
  Y. Xiong and B. R. Oakley In vivo analysis of the functions of {gamma}-tubulin-complex proteins J. Cell Sci., November 15, 2009; 122(22): 4218 - 4227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.J. T. Zeng, Y.-R. J. Lee, and B. Liu The WD40 Repeat Protein NEDD1 Functions in Microtubule Organization during Cell Division in Arabidopsis thaliana PLANT CELL, April 1, 2009; 21(4): 1129 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Guo, C.-M. K. Ho, Z. Kong, Y.-R. J. Lee, Q. Qian, and B. Liu Evaluating the microtubule cytoskeleton and its interacting proteins in monocots by mining the rice genome Ann. Bot., February 1, 2009; 103(3): 387 - 402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Armer, K. Moffat, T. Wileman, G. J. Belsham, T. Jackson, W. P. Duprex, M. Ryan, and P. Monaghan Foot-and-Mouth Disease Virus, but Not Bovine Enterovirus, Targets the Host Cell Cytoskeleton via the Nonstructural Protein 3Cpro J. Virol., November 1, 2008; 82(21): 10556 - 10566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Izumi, K. Fumoto, S. Izumi, and A. Kikuchi GSK-3{beta} Regulates Proper Mitotic Spindle Formation in Cooperation with a Component of the {gamma}-Tubulin Ring Complex, GCP5 J. Biol. Chem., May 9, 2008; 283(19): 12981 - 12991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Cunningham and R. A. Kahn Cofactor D Functions as a Centrosomal Protein and Is Required for the Recruitment of the {gamma}-Tubulin Ring Complex at Centrosomes and Organization of the Mitotic Spindle J. Biol. Chem., March 14, 2008; 283(11): 7155 - 7165. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Wiese Distinct Dgrip84 Isoforms Correlate with Distinct {gamma}-Tubulins in Drosophila Mol. Biol. Cell, January 1, 2008; 19(1): 368 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hoppeler-Lebel, C. Celati, G. Bellett, M. M. Mogensen, L. Klein-Hitpass, M. Bornens, and A.-M. Tassin Centrosomal CAP350 protein stabilises microtubules associated with the Golgi complex J. Cell Sci., September 15, 2007; 120(18): 3299 - 3308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Oriolo, F. A. Wald, G. Canessa, and P. J.I. Salas GCP6 Binds to Intermediate Filaments: A Novel Function of Keratins in the Organization of Microtubules in Epithelial Cells Mol. Biol. Cell, March 1, 2007; 18(3): 781 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Anders, P. C.C. Lourenco, and K. E. Sawin Noncore Components of the Fission Yeast {gamma}-Tubulin Complex Mol. Biol. Cell, December 1, 2006; 17(12): 5075 - 5093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wiese and Y. Zheng Microtubule nucleation: {gamma}-tubulin and beyond J. Cell Sci., October 15, 2006; 119(20): 4143 - 4153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Vogt, I. Koch, H. Schwarz, F. Schnorrer, and C. Nusslein-Volhard The {gamma}TuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline Development, October 15, 2006; 133(20): 3963 - 3972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ferralli, J. Ashby, M. Fasler, V. Boyko, and M. Heinlein Disruption of Microtubule Organization and Centrosome Function by Expression of Tobacco Mosaic Virus Movement Protein J. Virol., June 15, 2006; 80(12): 5807 - 5821. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Wang, X. Du, J. Meinkoth, Y. Hirohashi, H. Zhang, Q. Liu, M. Richter, and M. I. Greene Characterization of Su48, a centrosome protein essential for cell division PNAS, April 25, 2006; 103(17): 6512 - 6517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Masuda, T. Toda, R. Miyamoto, T. Haraguchi, and Y. Hiraoka Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic {gamma}-tubulin complexes Genes Cells, April 1, 2006; 11(4): 319 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Haren, M.-H. Remy, I. Bazin, I. Callebaut, M. Wright, and A. Merdes NEDD1-dependent recruitment of the {gamma}-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly J. Cell Biol., February 13, 2006; 172(4): 505 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Verollet, N. Colombie, T. Daubon, H.-M. Bourbon, M. Wright, and B. Raynaud-Messina Drosophila melanogaster {gamma}-TuRC is dispensable for targeting {gamma}-tubulin to the centrosome and microtubule nucleation J. Cell Biol., February 13, 2006; 172(4): 517 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Colombie, C. Verollet, P. Sampaio, A. Moisand, C. Sunkel, H.-M. Bourbon, M. Wright, and B. Raynaud-Messina The Drosophila {gamma}-Tubulin Small Complex Subunit Dgrip84 Is Required for Structural and Functional Integrity of the Spindle Apparatus Mol. Biol. Cell, January 1, 2006; 17(1): 272 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zimmerman and F. Chang Effects of {gamma}-Tubulin Complex Proteins on Microtubule Nucleation and Catastrophe in Fission Yeast Mol. Biol. Cell, June 1, 2005; 16(6): 2719 - 2733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Delgehyr, J. Sillibourne, and M. Bornens Microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function J. Cell Sci., April 15, 2005; 118(8): 1565 - 1575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Raynaud-Messina, L. Mazzolini, A. Moisand, A.-M. Cirinesi, and M. Wright Elongation of centriolar microtubule triplets contributes to the formation of the mitotic spindle in {gamma}-tubulin-depleted cells J. Cell Sci., November 1, 2004; 117(23): 5497 - 5507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. C. Zimmerman, J. Sillibourne, J. Rosa, and S. J. Doxsey Mitosis-specific Anchoring of {gamma} Tubulin Complexes by Pericentrin Controls Spindle Organization and Mitotic Entry Mol. Biol. Cell, August 1, 2004; 15(8): 3642 - 3657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tange, A. Fujita, T. Toda, and O. Niwa Functional Dissection of the {gamma}-Tubulin Complex by Suppressor Analysis of gtb1 and alp4 Mutations in Schizosaccharomyces pombe Genetics, July 1, 2004; 167(3): 1095 - 1107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Venkatram, J. J. Tasto, A. Feoktistova, J. L. Jennings, A. J. Link, and K. L. Gould Identification and Characterization of Two Novel Proteins Affecting Fission Yeast {gamma}-tubulin Complex Function Mol. Biol. Cell, May 1, 2004; 15(5): 2287 - 2301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-i. Kawaguchi and Y. Zheng Characterization of a Drosophila Centrosome Protein CP309 That Shares Homology with Kendrin and CG-NAP Mol. Biol. Cell, January 1, 2004; 15(1): 37 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Keryer, B. Di Fiore, C. Celati, K. F. Lechtreck, M. Mogensen, A. Delouvee, P. Lavia, M. Bornens, and A.-M. Tassin Part of Ran Is Associated with AKAP450 at the Centrosome: Involvement in Microtubule-organizing Activity Mol. Biol. Cell, October 1, 2003; 14(10): 4260 - 4271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. N. Gunawardane, O. C. Martin, and Y. Zheng Characterization of a New gamma TuRC Subunit with WD Repeats Mol. Biol. Cell, March 1, 2003; 14(3): 1017 - 1026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takahashi, A. Yamagiwa, T. Nishimura, H. Mukai, and Y. Ono Centrosomal Proteins CG-NAP and Kendrin Provide Microtubule Nucleation Sites by Anchoring gamma -Tubulin Ring Complex Mol. Biol. Cell, September 1, 2002; 13(9): 3235 - 3245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Fujita, L. Vardy, M. A. Garcia, and T. Toda A Fourth Component of the Fission Yeast gamma -Tubulin Complex, Alp16, Is Required for Cytoplasmic Microtubule Integrity and Becomes Indispensable When gamma -Tubulin Function Is Compromised Mol. Biol. Cell, July 1, 2002; 13(7): 2360 - 2373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B.N. Vinh, J. W. Kern, W. O. Hancock, J. Howard, and T. N. Davis Reconstitution and Characterization of Budding Yeast gamma -Tubulin Complex Mol. Biol. Cell, April 1, 2002; 13(4): 1144 - 1157. [Abstract] [Full Text] [PDF]
|
|
