![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 11, 3365-3374, November 2001
B
Promoter
within Chromatin
and
The glucocorticoid receptor (GR) is a ligand-activated
transcription factor that induces expression of many genes. The GR has
been useful for understanding how chromatin structure regulates steroid-induced transcription in model systems. However, the effect of
glucocorticoids on chromatin structure has been examined on few
endogenous mammalian promoters. We investigated the effect of
glucocorticoids on the in vivo chromatin structure of the
glucocorticoid-responsive IChromatin and Gene Expression Section, National
Institute of Environmental Health Sciences, National Institutes of
Health, Research Triangle Park, North Carolina 27709; and *Department
of Biochemistry, The University of Western Ontario, London, Ontario,
N6A 4L6, Canada.
B
gene promoter, the inhibitor of the
ubiquitous transcription factor, nuclear factor kappa B (NFB).
Glucocorticoids inhibit NFB activity in some tissues by elevating
the levels of IB. We found that glucocorticoids activated the
IB promoter in human T47D/A1-2 cells containing the GR. We then
investigated the chromatin structure of the IB promoter in the
absence and presence of glucocorticoids with the use of micrococcal
nuclease, restriction enzyme, and deoxyribonuclease (DNaseI) analyses.
In untreated cells, the promoter assembles into regularly positioned
nucleosomes, and glucocorticoid treatment did not alter nucleosomal
position. Restriction enzyme accessiblity studies indicated that the
IB promoter is assembled as phased nucleosomes that adopt an
"open" chromatin architecture in the absence of hormone. However,
glucocorticoids may be required for transcription factor binding,
because DNaseI footprinting studies suggested that regulatory factors
bind to the promoter upon glucocorticoid treatment.
Corresponding author. E-mail address:
archer1{at}niehs.nih.gov.
This article has been cited by other articles:
|
|
 |
|
  P. B. Hebbar and T. K. Archer Altered Histone H1 Stoichiometry and an Absence of Nucleosome Positioning on Transfected DNA J. Biol. Chem., February 22, 2008; 283(8): 4595 - 4601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Hardy, B. A. Janowski, D. R. Corey, and C. R. Mendelson Progesterone Receptor Plays a Major Antiinflammatory Role in Human Myometrial Cells by Antagonism of Nuclear Factor-{kappa}B Activation of Cyclooxygenase 2 Expression Mol. Endocrinol., November 1, 2006; 20(11): 2724 - 2733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Paulus, P. J. Sollars, G. E. Pickard, and L. W. Enquist Transcriptome Signature of Virulent and Attenuated Pseudorabies Virus-Infected Rodent Brain J. Virol., February 15, 2006; 80(4): 1773 - 1786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Xing, D. C. Wilkerson, C. N. Mayhew, E. J. Lubert, H. S. Skaggs, M. L. Goodson, Y. Hong, O.-K. Park-Sarge, and K. D. Sarge Mechanism of hsp70i Gene Bookmarking Science, January 21, 2005; 307(5708): 421 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. W. Trotter and T. K. Archer Reconstitution of Glucocorticoid Receptor-Dependent Transcription In Vivo Mol. Cell. Biol., April 15, 2004; 24(8): 3347 - 3358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. De Bosscher, W. Vanden Berghe, and G. Haegeman The Interplay between the Glucocorticoid Receptor and Nuclear Factor-{kappa}B or Activator Protein-1: Molecular Mechanisms for Gene Repression Endocr. Rev., August 1, 2003; 24(4): 488 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. B. Hebbar and T. K. Archer Nuclear Factor 1 Is Required for Both Hormone-Dependent Chromatin Remodeling and Transcriptional Activation of the Mouse Mammary Tumor Virus Promoter Mol. Cell. Biol., February 1, 2003; 23(3): 887 - 898. [Abstract] [Full Text]
|
|
