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Vol. 12, Issue 11, 3476-3488, November 2001
and
*Department of Molecular, Cellular, and Developmental Biology,
University of Colorado, Boulder, Colorado 80309-0347;
We have identified Klp2p, a new kinesin-like protein (KLP) of the
KAR3 subfamily in fission yeast. The motor domain of this protein is
61% identical and 71% similar to Pkl1p, another fission yeast KAR3
protein, yet the two enzymes are different in behavior and
function. Pkl1p is nuclear throughout the cell cycle, whereas Klp2p is
cytoplasmic during interphase. During mitosis Klp2p enters the nucleus
where it forms about six chromatin-associated dots. In
metaphase-arrested cells these migrate back and forth across the
nucleus. During early anaphase they segregate with the chromosomes into
two sets of about three, fade, and are replaced by other dots that form
on the spindle interzone. Neither
klp2+ nor
pkl1+ is essential, and the double
deletion is also wild type for both vegetative and sexual reproduction.
Each deletion rescues different alleles of
cut7ts, a KLP that contributes to
spindle formation and elongation. When either or both deletions are
combined with a dynein deletion, vegetative growth is normal, but
sexual reproduction fails: klp2Department of Molecular and Cellular Biology, University
of California, Berkeley, California 94720; and Human
Genetics Unit, Edinburgh, Scotland EH4 2XU
,dhc1-d1 in
karyogamy, pkl1,dhc1-d1 in multiple
phases of meiosis, and the triple deletion in both. Deletion of Klp2p
elongates a metaphase-arrested spindle, but pkl1
shortens it. The anaphase spindle of klp2 becomes
longer than the cell, leading it to curl around the cell's ends.
Apparently, Klp2p promotes spindle disassembly and contributes to the
behavior of mitotic chromosomes.
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