![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 11, 3489-3501, November 2001
§ and
*Program in Physiology, Biophysics, and Molecular Medicine and
In several cell types, specific membrane proteins are retained
intracellularly and rapidly redistributed to the surface in response to
stimulation. In fat and muscle, the GLUT4 glucose transporter is
dynamically retained because it is rapidly internalized and slowly
recycled to the plasma membrane. Insulin increases the recycling of
GLUT4, resulting in a net translocation to the surface. We have shown
that fibroblasts also have an insulin-regulated recycling mechanism.
Here we show that GLUT4 is retained within the transferrin
receptor-containing general endosomal recycling compartment in Chinese
hamster ovary (CHO) cells rather than being segregated to a
specialized, GLUT4-recycling compartment. With the use of total
internal reflection microscopy, we demonstrate that the TR and GLUT4
are transported from the pericentriolar recycling compartment in
separate vesicles. These data provide the first functional evidence for
the formation of distinct classes of vesicles from the recycling
compartment. We propose that GLUT4 is dynamically retained within the
endosomal recycling compartment in CHO cells because it is concentrated
in vesicles that form more slowly than those that transport TR. In
3T3-L1 adipocytes, cells that naturally express GLUT4, we find that
GLUT4 is partially segregated to a separate compartment that is
inaccessible to the TR. We present a model for the formation of this
specialized compartment in fat cells, based on the general mechanism
described in CHO cells, which may explain the increased retention of
GLUT4 and its insulin-induced translocation in fat cells.
Department of Biochemistry, Weill Graduate School of
Medical Sciences of Cornell University, New York, New York 10021;
§Institute of Zellenlehre, University of Heidelberg,
Heidelberg, Germany D-69117; and Laboratory of
Cellular Biophysics, The Rockefeller University, New York, New York
10021
Corresponding author. E-mail address:
temcgraw{at}mail.med.cornell.edu.
This article has been cited by other articles:
|
|
 |
|
  J. K. Jaiswal, M. Fix, T. Takano, M. Nedergaard, and S. M. Simon Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes PNAS, August 28, 2007; 104(35): 14151 - 14156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-B. Liu, W. Omata, I. Kojima, and H. Shibata The SUMO Conjugating Enzyme Ubc9 is a Regulator of GLUT4 Turnover and Targeting to the Insulin-Responsive Storage Compartment in 3T3-L1 Adipocytes Diabetes, August 1, 2007; 56(8): 1977 - 1985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Huang, L. M. Lifshitz, C. Jones, K. D. Bellve, C. Standley, S. Fonseca, S. Corvera, K. E. Fogarty, and M. P. Czech Insulin Stimulates Membrane Fusion and GLUT4 Accumulation in Clathrin Coats on Adipocyte Plasma Membranes Mol. Cell. Biol., May 1, 2007; 27(9): 3456 - 3469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Prabhat, Z. Gan, J. Chao, S. Ram, C. Vaccaro, S. Gibbons, R. J. Ober, and E. S. Ward Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy PNAS, April 3, 2007; 104(14): 5889 - 5894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Muretta, I. Romenskaia, P. A. Cassiday, and C. C. Mastick Expression of a synapsin IIb site 1 phosphorylation mutant in 3T3-L1 adipocytes inhibits basal intracellular retention of Glut4 J. Cell Sci., April 1, 2007; 120(7): 1168 - 1177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Hou, N. Suzuki, J. E. Pessin, and R. T. Watson A Specific Dileucine Motif Is Required for the GGA-dependent Entry of Newly Synthesized Insulin-responsive Aminopeptidase into the Insulin-responsive Compartment J. Biol. Chem., November 3, 2006; 281(44): 33457 - 33466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Z. Rappoport, S. Kemal, A. Benmerah, and S. M. Simon Dynamics of clathrin and adaptor proteins during endocytosis Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1072 - C1081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Gonzalez and T. E. McGraw Insulin Signaling Diverges into Akt-dependent and -independent Signals to Regulate the Recruitment/Docking and the Fusion of GLUT4 Vesicles to the Plasma Membrane Mol. Biol. Cell, October 1, 2006; 17(10): 4484 - 4493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mari, P. Monzo, V. Kaddai, F. Keslair, T. Gonzalez, Y. Le Marchand-Brustel, and M. Cormont The Rab4 effector Rabip4 plays a role in the endocytotic trafficking of Glut 4 in 3T3-L1 adipocytes J. Cell Sci., April 1, 2006; 119(7): 1297 - 1306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. J. Martin, A. Lee, and T. E. McGraw GLUT4 Distribution between the Plasma Membrane and the Intracellular Compartments Is Maintained by an Insulin-modulated Bipartite Dynamic Mechanism J. Biol. Chem., January 6, 2006; 281(1): 484 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Strick and L. A. Elferink Rab15 Effector Protein: A Novel Protein for Receptor Recycling from the Endocytic Recycling Compartment Mol. Biol. Cell, December 1, 2005; 16(12): 5699 - 5709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. S. L. Thong, C. B. Dugani, and A. Klip Turning Signals On and Off: GLUT4 Traffic in the Insulin-Signaling Highway Physiology, August 1, 2005; 20(4): 271 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Smith, J. T. Cirulis, J. E. Casanova, M. A. Scidmore, and J. H. Brumell Interaction of the Salmonella-containing Vacuole with the Endocytic Recycling System J. Biol. Chem., July 1, 2005; 280(26): 24634 - 24641. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A. Lizunov, H. Matsumoto, J. Zimmerberg, S. W. Cushman, and V. A. Frolov Insulin stimulates the halting, tethering, and fusion of mobile GLUT4 vesicles in rat adipose cells J. Cell Biol., May 9, 2005; 169(3): 481 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Eyster, Q. S. Duggins, and A. L. Olson Expression of Constitutively Active Akt/Protein Kinase B Signals GLUT4 Translocation in the Absence of an Intact Actin Cytoskeleton J. Biol. Chem., May 6, 2005; 280(18): 17978 - 17985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Wertheim, Z. Cai, and T. E. McGraw The Transcription Factor CCAAT/Enhancer-binding Protein {alpha} Is Required for the Intracellular Retention of GLUT4 J. Biol. Chem., October 1, 2004; 279(40): 41468 - 41476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zeigerer, M. K. McBrayer, and T. E. McGraw Insulin Stimulation of GLUT4 Exocytosis, but Not Its Inhibition of Endocytosis, Is Dependent on RabGAP AS160 Mol. Biol. Cell, October 1, 2004; 15(10): 4406 - 4415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Guilherme, N. A. Soriano, P. S. Furcinitti, and M. P. Czech Role of EHD1 and EHBP1 in Perinuclear Sorting and Insulin-regulated GLUT4 Recycling in 3T3-L1 Adipocytes J. Biol. Chem., September 17, 2004; 279(38): 40062 - 40075. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Malikova, J. Shi, and K. V. Kandror V-type ATPase is involved in biogenesis of GLUT4 vesicles Am J Physiol Endocrinol Metab, September 1, 2004; 287(3): E547 - E552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Ober, C. Martinez, X. Lai, J. Zhou, and E. S. Ward Exocytosis of IgG as mediated by the receptor, FcRn: An analysis at the single-molecule level PNAS, July 27, 2004; 101(30): 11076 - 11081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanzaki, M. Furukawa, W. Raab, and J. E. Pessin Phosphatidylinositol 4,5-Bisphosphate Regulates Adipocyte Actin Dynamics and GLUT4 Vesicle Recycling J. Biol. Chem., July 16, 2004; 279(29): 30622 - 30633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Govers, A. C. F. Coster, and D. E. James Insulin Increases Cell Surface GLUT4 Levels by Dose Dependently Discharging GLUT4 into a Cell Surface Recycling Pathway Mol. Cell. Biol., July 15, 2004; 24(14): 6456 - 6466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Bauer, R. L. Overlease, J. L. Lieber, and J. K. Angleson Retention and stimulus-dependent recycling of dense core vesicle content in neuroendocrine cells J. Cell Sci., May 1, 2004; 117(11): 2193 - 2202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Keyel, S. C. Watkins, and L. M. Traub Endocytic Adaptor Molecules Reveal an Endosomal Population of Clathrin by Total Internal Reflection Fluorescence Microscopy J. Biol. Chem., March 26, 2004; 279(13): 13190 - 13204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Karylowski, A. Zeigerer, A. Cohen, and T. E. McGraw GLUT4 Is Retained by an Intracellular Cycle of Vesicle Formation and Fusion with Endosomes Mol. Biol. Cell, February 1, 2004; 15(2): 870 - 882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Z. Rappoport, B. W. Taha, S. Lemeer, A. Benmerah, and S. M. Simon The AP-2 Complex Is Excluded from the Dynamic Population of Plasma Membrane-associated Clathrin J. Biol. Chem., November 28, 2003; 278(48): 47357 - 47360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Olson, C. A. Eyster, Q. S. Duggins, and J. B. Knight Insulin Promotes Formation of Polymerized Microtubules by a Phosphatidylinositol 3-Kinase-Independent, Actin-Dependent Pathway in 3T3-L1 Adipocytes Endocrinology, November 1, 2003; 144(11): 5030 - 5039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rajagopal and S. M. Simon Subcellular Localization and Activity of Multidrug Resistance Proteins Mol. Biol. Cell, August 1, 2003; 14(8): 3389 - 3399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shigematsu, R. T. Watson, A. H. Khan, and J. E. Pessin The Adipocyte Plasma Membrane Caveolin Functional/Structural Organization Is Necessary for the Efficient Endocytosis of GLUT4 J. Biol. Chem., March 14, 2003; 278(12): 10683 - 10690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Z. Rappoport and S. M. Simon Real-time analysis of clathrin-mediated endocytosis during cell migration J. Cell Sci., March 1, 2003; 116(5): 847 - 855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Shewan, E. M. van Dam, S. Martin, T. B. Luen, W. Hong, N. J. Bryant, and D. E. James GLUT4 Recycles via a trans-Golgi Network (TGN) Subdomain Enriched in Syntaxins 6 and 16 But Not TGN38: Involvement of an Acidic Targeting Motif Mol. Biol. Cell, March 1, 2003; 14(3): 973 - 986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Jaiswal, N. W. Andrews, and S. M. Simon Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells J. Cell Biol., November 25, 2002; 159(4): 625 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zeigerer, M. A. Lampson, O. Karylowski, D. D. Sabatini, M. Adesnik, M. Ren, and T. E. McGraw GLUT4 Retention in Adipocytes Requires Two Intracellular Insulin-regulated Transport Steps Mol. Biol. Cell, July 1, 2002; 13(7): 2421 - 2435. [Abstract] [Full Text] [PDF]
|
|
