![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 11, 3527-3537, November 2001
§
Departments of *Molecular and Cellular Biology,
Condensin is a conserved 13S heteropentamer composed of two
nonidentical structural maintenance of chromosome (SMC) family proteins, in Xenopus XCAP-C and XCAP-E, and three
regulatory subunits, XCAP-D2, XCAP-G, and XCAP-H. Both biochemical and
genetic analyses have demonstrated an essential role for the 13S
condensin complex in mitotic chromosome condensation. Further, a
potential requirement for condensin in completion of chromatid arm
separation in early anaphase is demonstrated by the mutational
phenotypes of the Drosophila homologues of
XCAP-H, barren and XCAP-C,
DmSMC4. In this study we have investigated the
expression and subcellular distribution of hCAP-H, the human homolog of
XCAP-H, in order to better understand its cellular functions.
Transcription of hCAP-H was restricted to proliferating
cells with highest expression during the G2 phase of the
cell cycle. In contrast, cellular hCAP-H protein levels were constant
throughout the cell cycle. hCAP-H was found to be associated with
mitotic chromosomes exhibiting a nonuniform but symmetric distribution
along sister chromatids. The symmetry of hCAP-H association with sister
chromatids suggests that there are sequence-dependent domains of
condensin aggregation. During interphase hCAP-H, -C, and -E, have
distinct punctate nucleolar localization, suggesting that condensin may
associate with and modulate the conformation and function of rDNA.
hCAP-H association with condensed chromatin was not observed in the
early phase of chromosome condensation when histone H3 phosphorylation
has already taken place. This finding is consistent with the hypothesis
that histone H3 phosphorylation precedes condensin-mediated condensation.
Molecular and Human Genetics, Pediatrics,
and §Immunology, Baylor College of Medicine, One Baylor
Plaza, Houston, Texas 77030
Corresponding author. E-mail address:
jbelmont{at}bcm.tmc.edu.
This article has been cited by other articles:
|
|
 |
|
  N. Nakazawa, T. Nakamura, A. Kokubu, M. Ebe, K. Nagao, and M. Yanagida Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis J. Cell Biol., March 24, 2008; 180(6): 1115 - 1131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Scibetta, S. Santangelo, J. Coleman, D. Hall, T. Chaplin, J. Copier, S. Catchpole, J. Burchell, and J. Taylor-Papadimitriou Functional Analysis of the Transcription Repressor PLU-1/JARID1B Mol. Cell. Biol., October 15, 2007; 27(20): 7220 - 7235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Blank, Y. Lerenthal, L. Mittelman, and Y. Shiloh Condensin I recruitment and uneven chromatin condensation precede mitotic cell death in response to DNA damage J. Cell Biol., July 17, 2006; 174(2): 195 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Johzuka, M. Terasawa, H. Ogawa, T. Ogawa, and T. Horiuchi Condensin Loaded onto the Replication Fork Barrier Site in the rRNA Gene Repeats during S Phase in a FOB1-Dependent Fashion To Prevent Contraction of a Long Repetitive Array in Saccharomyces cerevisiae. Mol. Cell. Biol., March 1, 2006; 26(6): 2226 - 2236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Cobbe, E. Savvidou, and M. M. S. Heck Diverse Mitotic and Interphase Functions of Condensins in Drosophila Genetics, February 1, 2006; 172(2): 991 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-D. Wang, D. Eyre, M. Basrai, M. Lichten, and A. Strunnikov Condensin Binding at Distinct and Specific Chromosomal Sites in the Saccharomyces cerevisiae Genome Mol. Cell. Biol., August 15, 2005; 25(16): 7216 - 7225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hirota, D. Gerlich, B. Koch, J. Ellenberg, and J.-M. Peters Distinct functions of condensin I and II in mitotic chromosome assembly J. Cell Sci., December 15, 2004; 117(26): 6435 - 6445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Takemoto, K. Kimura, S. Yokoyama, and F. Hanaoka Cell Cycle-dependent Phosphorylation, Nuclear Localization, and Activation of Human Condensin J. Biol. Chem., February 6, 2004; 279(6): 4551 - 4559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Uzbekov, E. Timirbulatova, E. Watrin, F. Cubizolles, D. Ogereau, P. Gulak, V. Legagneux, V. Ju. Polyakov, K. Le Guellec, and I. Kireev Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells J. Cell Sci., May 1, 2003; 116(9): 1667 - 1678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Przewloka, P. E. Pardington, S. M. Yannone, D. J. Chen, and R. B. Cary In Vitro and In Vivo Interactions of DNA Ligase IV with a Subunit of the Condensin Complex Mol. Biol. Cell, February 1, 2003; 14(2): 685 - 697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Ball Jr., J. A. Schmiesing, C. Zhou, H. C. Gregson, Y. Okada, T. Doi, and K. Yokomori Identification of a Chromosome-Targeting Domain in the Human Condensin Subunit CNAP1/hCAP-D2/Eg7 Mol. Cell. Biol., August 15, 2002; 22(16): 5769 - 5781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hirano The ABCs of SMC proteins: two-armed ATPases for chromosome condensation, cohesion, and repair Genes & Dev., February 15, 2002; 16(4): 399 - 414. [Full Text] [PDF]
|
|
