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Vol. 12, Issue 11, 3538-3549, November 2001
and§
*Department of Pediatrics, University of Minnesota, Minneapolis,
Minnesota 55455; The ability to switch between yeast and hyphal morphologies is an
important virulence factor for the opportunistic pathogen Candida albicans. Although the kinetics of appearance of
the filamentous ring that forms at the incipient septum differ in yeast
and cells forming hyphae (germ tubes) (Soll and Mitchell, 1983), the
molecular mechanisms that regulate this difference are not known.
Int1p, a C. albicans gene product with similarity in its
C terminus to Saccharomyces cerevisiae Bud4p, has a role
in hyphal morphogenesis. Here we report that in S.
cerevisiae, Int1p expression results in the growth of highly
polarized cells with delocalized chitin and defects in cytokinesis and
bud-site selection patterns, phenotypes that are also seen in S.
cerevisiae septin mutant strains. Expression of high levels of
Int1p in S. cerevisiae generated elaborate spiral-like structures at the periphery of the polarized cells that contained septins and Int1p. In addition, Int1p coimmunoprecipitated with the
Cdc11p and Cdc12p septins, and Cdc12p is required for the establishment
and maintenance of these Int1p/septin spirals. Although Swe1p kinase
contributes to INT1-induced filamentous growth in S. cerevisiae, it is not required for the formation of
ectopic Int1p/septin structures. In C. albicans, Int1p
was important for the axial budding pattern and colocalized with Cdc3p
septin in a ring at the mother-bud neck of yeast and pseudohyphal
cells. Under conditions that induce hyphae, both Cdc3p and Int1p
localized to a ring distal to the junction of the mother cell and germ
tube. Thus, placement of the Int1p/septin ring with respect to the
mother-daughter cell junction distinguishes yeast/pseudohyphal growth
from hyphal growth in C. albicans.
Department of Genetics, Cell Biology and
Development, University of Minnesota, St. Paul, Minnesota 55108; and
Department of Biochemistry and Molecular Biology,
Oklahoma State University, Stillwater, Oklahoma 74078
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