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Vol. 12, Issue 11, 3644-3657, November 2001

Novel Stress-responsive Genes EMG1 and NOP14 Encode Conserved, Interacting Proteins Required for 40S Ribosome Biogenesis

Phillip C. C. Liu,^* and Dennis J. Thiele

Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, Michigan 48109-0606

Under stressful conditions organisms adjust the synthesis, processing, and trafficking of molecules to allow survival from and recovery after stress. In baker's yeast Saccharomyces cerevisiae, the cellular production of ribosomes is tightly matched with environmental conditions and nutrient availability through coordinate transcriptional regulation of genes involved in ribosome biogenesis. On the basis of stress-responsive gene expression and functional studies, we have identified a novel, evolutionarily conserved gene, EMG1, that has similar stress-responsive gene expression patterns as ribosomal protein genes and is required for the biogenesis of the 40S ribosomal subunit. The Emg1 protein is distributed throughout the cell; however, its nuclear localization depends on physical interaction with a newly characterized nucleolar protein, Nop14. Yeast depleted of Nop14 or harboring a temperature-sensitive allele of emg1 have selectively reduced levels of the 20S pre-rRNA and mature18S rRNA and diminished cellular levels of the 40S ribosomal subunit. Neither Emg1 nor Nop14 contain any characterized functional motifs; however, isolation and functional analyses of mammalian orthologues of Emg1 and Nop14 suggest that these proteins are functionally conserved among eukaryotes. We conclude that Emg1 and Nop14 are novel proteins whose interaction is required for the maturation of the 18S rRNA and for 40S ribosome production.

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^* Present address: Applied Biotechnology, Dupont Pharmaceuticals Co., P.O. Box 80, Bldg. 336, Wilmington, DE 19880-0361.

Corresponding author. E-mail address: dthiele{at}umich.edu.

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Molecular Biology of the Cell
Vol. 12, 3644-3657, November 2001
Copyright © 2001 by The American Society for Cell Biology

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