![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 12, 3892-3903, December 2001
,*
and
*Departments of Biology and In budding yeast, actin disruption prevents nuclear division. This
has been explained as activation of a morphogenesis checkpoint monitoring the integrity of the actin cytoskeleton. The checkpoint operates through inhibitory tyrosine phosphorylation of Cdc28, the
budding yeast Cdc2 homolog. Wild-type Schizosaccharomyces pombe cells also arrest before mitosis after actin
depolymerization. Oversized cells, however, enter mitosis uninhibited.
We carried out a careful analysis of the kinetics of mitotic initiation
after actin disruption in undersized and oversized cells. We show that an inability to reach the mitotic size threshold explains the arrest in
smaller cells. Among the regulators that control the level of the
inhibitory Cdc2-Tyr15 phosphorylation, the Cdc25 protein tyrosine
phosphatase is required to link cell size monitoring to mitotic
control. This represents a novel function of the Cdc25 phosphatase.
Furthermore, we demonstrate that this cell size-monitoring system
fulfills the formal criteria of a cell cycle checkpoint.
Biochemistry, Queen's
University, Kingston, Ontario K7L 3N6, Canada
Corresponding author. E-mail address:
youngpg{at}biology.queensu.ca.
This article has been cited by other articles:
|
|
 |
|
  R. R. Daga and P. Nurse Interphase microtubule bundles use global cell shape to guide spindle alignment in fission yeast J. Cell Sci., June 15, 2008; 121(12): 1973 - 1980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. T. George, G. Brooks, and T. C. Humphrey Regulation of Cell Cycle and Stress Responses to Hydrostatic Pressure in Fission Yeast Mol. Biol. Cell, October 1, 2007; 18(10): 4168 - 4179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Douglas, F. J. Alvarez, C. McCreary, and J. B. Konopka Septin Function in Yeast Model Systems and Pathogenic Fungi Eukaryot. Cell, September 1, 2005; 4(9): 1503 - 1512. [Full Text] [PDF]
|
|
|
|
|
|
J. Karagiannis, A. Bimbo, S. Rajagopalan, J. Liu, and M. K. Balasubramanian The Nuclear Kinase Lsk1p Positively Regulates the Septation Initiation Network and Promotes the Successful Completion of Cytokinesis in Response to Perturbation of the Actomyosin Ring in Schizosaccharomyces pombe Mol. Biol. Cell, January 1, 2005; 16(1): 358 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tournier, Y. Gachet, V. Buck, J. S. Hyams, and J. B.A. Millar Disruption of Astral Microtubule Contact with the Cell Cortex Activates a Bub1, Bub3, and Mad3-dependent Checkpoint in Fission Yeast Mol. Biol. Cell, July 1, 2004; 15(7): 3345 - 3356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Venkatram, J. J. Tasto, A. Feoktistova, J. L. Jennings, A. J. Link, and K. L. Gould Identification and Characterization of Two Novel Proteins Affecting Fission Yeast {gamma}-tubulin Complex Function Mol. Biol. Cell, May 1, 2004; 15(5): 2287 - 2301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Kellogg Wee1-dependent mechanisms required for coordination of cell growth and cell division J. Cell Sci., December 15, 2003; 116(24): 4883 - 4890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Coll, Y. Trillo, A. Ametzazurra, and P. Perez Gef1p, a New Guanine Nucleotide Exchange Factor for Cdc42p, Regulates Polarity in Schizosaccharomyces pombe Mol. Biol. Cell, January 1, 2003; 14(1): 313 - 323. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Chua, C. Lingner, C. Frazer, and P. G. Young The sal3+ Gene Encodes an Importin-{beta} Implicated in the Nuclear Import of Cdc25 in Schizosaccharomyces pombe Genetics, October 1, 2002; 162(2): 689 - 703. [Abstract] [Full Text] [PDF]
|
|
