QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yamamoto, A. Articles by Hiraoka, Y. Search for Related Content PubMed PubMed Citation Articles by Yamamoto, A. Articles by Hiraoka, Y.

Vol. 12, Issue 12, 3933-3946, December 2001

Dynamic Behavior of Microtubules during Dynein-dependent Nuclear Migrations of Meiotic Prophase in Fission Yeast

Ayumu Yamamoto,^* Chihiro Tsutsumi, Hiroaki Kojima,^§ Kazuhiro Oiwa,^§ and Yasushi Hiraoka^*

 ^*Cell Biology Group,  CREST Research Project, and  ^§Protein Biophysics Group, Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651-2492, Japan

During meiotic prophase in fission yeast, the nucleus migrates back and forth between the two ends of the cell, led by the spindle pole body (SPB). This nuclear oscillation is dependent on astral microtubules radiating from the SPB and a microtubule motor, cytoplasmic dynein. Here we have examined the dynamic behavior of astral microtubules labeled with the green fluorescent protein during meiotic prophase with the use of optical sectioning microscopy. During nuclear migrations, the SPB mostly follows the microtubules that extend toward the cell cortex. SPB migrations start when these microtubules interact with the cortex and stop when they disappear, suggesting that these microtubules drive nuclear migrations. The microtubules that are followed by the SPB often slide along the cortex and are shortened by disassembly at their ends proximal to the cortex. In dynein-mutant cells, where nuclear oscillations are absent, the SPB never migrates by following microtubules, and microtubule assembly/disassembly dynamics is significantly altered. Based on these observations, together with the frequent accumulation of dynein at a cortical site where the directing microtubules interact, we propose a model in which dynein drives nuclear oscillation by mediating cortical microtubule interactions and regulating the dynamics of microtubule disassembly at the cortex.

---

Corresponding author. E-mail address: ayumu{at}crl.go.jp.

---

Molecular Biology of the Cell
Vol. 12, 3933-3946, December 2001
Copyright © 2001 by The American Society for Cell Biology

This article has been cited by other articles:

				A. Yamamoto, K. Kitamura, D. Hihara, Y. Hirose, S. Katsuyama, and Y. Hiraoka Spindle checkpoint activation at meiosis I advances anaphase II onset via meiosis-specific APC/C regulation J. Cell Biol., July 28, 2008; 182(2): 277 - 288. [Abstract] [Full Text] [PDF]

				C. Couwenbergs, J.-C. Labbe, M. Goulding, T. Marty, B. Bowerman, and M. Gotta Heterotrimeric G protein signaling functions with dynein to promote spindle positioning in C. elegans J. Cell Biol., October 8, 2007; 179(1): 15 - 22. [Abstract] [Full Text] [PDF]

				A. Yamashita and M. Yamamoto Fission Yeast Num1p Is a Cortical Factor Anchoring Dynein and Is Essential for the Horse-Tail Nuclear Movement During Meiotic Prophase Genetics, July 1, 2006; 173(3): 1187 - 1196. [Abstract] [Full Text] [PDF]

				T. T. Saito, D. Okuzaki, and H. Nojima Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast J. Cell Biol., April 10, 2006; 173(1): 27 - 33. [Abstract] [Full Text] [PDF]

				H. Masuda, R. Miyamoto, T. Haraguchi, and Y. Hiraoka The carboxy-terminus of Alp4 alters microtubule dynamics to induce oscillatory nuclear movement led by the spindle pole body in Schizosaccharomyces pombe Genes Cells, April 1, 2006; 11(4): 337 - 352. [Abstract] [Full Text] [PDF]

				T. T. Saito, T. Tougan, D. Okuzaki, T. Kasama, and H. Nojima Mcp6, a meiosis-specific coiled-coil protein of Schizosaccharomyces pombe, localizes to the spindle pole body and is required for horsetail movement and recombination J. Cell Sci., January 15, 2005; 118(2): 447 - 459. [Abstract] [Full Text] [PDF]

				T. Niccoli, A. Yamashita, P. Nurse, and M. Yamamoto The p150-Glued Ssm4p regulates microtubular dynamics and nuclear movement in fission yeast J. Cell Sci., November 1, 2004; 117(23): 5543 - 5556. [Abstract] [Full Text] [PDF]

				J. L. Paluh, A. N. Killilea, H. W. Detrich 3rd, and K. H. Downing Meiosis-specific Failure of Cell Cycle Progression in Fission Yeast by Mutation of a Conserved {beta}-Tubulin Residue Mol. Biol. Cell, March 1, 2004; 15(3): 1160 - 1171. [Abstract] [Full Text] [PDF]

				A. Yamamoto and Y. Hiraoka Cytoplasmic dynein in fungi: insights from nuclear migration J. Cell Sci., November 15, 2003; 116(22): 4501 - 4512. [Abstract] [Full Text] [PDF]

				V. P. Efimov Roles of NUDE and NUDF Proteins of Aspergillus nidulans: Insights from Intracellular Localization and Overexpression Effects Mol. Biol. Cell, March 1, 2003; 14(3): 871 - 888. [Abstract] [Full Text] [PDF]

				F. Miki, K. Okazaki, M. Shimanuki, A. Yamamoto, Y. Hiraoka, and O. Niwa The 14-kDa Dynein Light Chain-Family Protein Dlc1 Is Required for Regular Oscillatory Nuclear Movement and Efficient Recombination during Meiotic Prophase in Fission Yeast Mol. Biol. Cell, March 1, 2002; 13(3): 930 - 946. [Abstract] [Full Text] [PDF]