![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 2, 279-295, February 2001
-Subunit Is Required for Epithelial Polarization,
Suppression of Invasion, and Cell Motility
*Department of Pathology and Laboratory Medicine, University of
California, Los Angeles, Los Angeles, California 90095; Departments of
The cell adhesion molecule E-cadherin has been implicated in
maintaining the polarized phenotype of epithelial cells and suppression of invasiveness and motility of carcinoma cells. Na,K-ATPase, consisting of an Physiology and Urology, Weill Medical
College of Cornell University, New York, New York 10021;
Department of Cell Biology, The Scripps Research
Institute, La Jolla, California 92037; §Department of
Pharmacology and Cell Biophysics, University of Cincinnati Medical
Center, Cincinnati, Ohio 45267; and ¶Lankenau Medical
Research Center, Wynnewood, Pennsylvania 19096
- and 
-subunit, maintains the sodium gradient across the plasma membrane. A functional relationship between E-cadherin and Na,K-ATPase has not previously been described. We
present evidence that the Na,K-ATPase plays a crucial role in
E-cadherin-mediated development of epithelial polarity, and suppression of invasiveness and motility of carcinoma cells. Moloney sarcoma virus-transformed Madin-Darby canine kidney cells (MSV-MDCK) have highly reduced levels of E-cadherin and 1-subunit
of Na,K-ATPase. Forced expression of E-cadherin in MSV-MDCK cells did
not reestablish epithelial polarity or inhibit the invasiveness and
motility of these cells. In contrast, expression of E-cadherin and
Na,K-ATPase 1-subunit induced epithelial polarization,
including the formation of tight junctions and desmosomes, abolished
invasiveness, and reduced cell motility in MSV-MDCK cells. Our results
suggest that E-cadherin-mediated cell-cell adhesion requires the
Na,K-ATPase -subunit's function to induce epithelial polarization
and suppress invasiveness and motility of carcinoma cells. Involvement
of the 1-subunit of Na,K-ATPase in the polarized
phenotype of epithelial cells reveals a novel link between the
structural organization and vectorial ion transport function of
epithelial cells.
This article has been cited by other articles:
|
|
 |
|
  R. J. Clifford and J. H. Kaplan Regulation of Na,K-ATPase Subunit Abundance by Translational Repression J. Biol. Chem., August 21, 2009; 284(34): 22905 - 22915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tian, X. Li, M. Liang, L. Liu, J. X. Xie, Q. Ye, P. Kometiani, M. Tillekeratne, R. Jin, and Z. Xie Changes in Sodium Pump Expression Dictate the Effects of Ouabain on Cell Growth J. Biol. Chem., May 29, 2009; 284(22): 14921 - 14929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bibert, D. Aebischer, F. Desgranges, S. Roy, D. Schaer, S. Kharoubi-Hess, J.-D. Horisberger, and K. Geering A Link between FXYD3 (Mat-8)-mediated Na,K-ATPase Regulation and Differentiation of Caco-2 Intestinal Epithelial Cells Mol. Biol. Cell, February 1, 2009; 20(4): 1132 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Clifford and J. H. Kaplan {beta}-Subunit overexpression alters the stoicheometry of assembled Na-K-ATPase subunits in MDCK cells Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1314 - F1323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Bouritius, D. C. van Hoorn, A. Oosting, M. C. van Middelaar-Voskuilen, C. J. P. van Limpt, K. J. Lamb, P. A. M. van Leeuwen, A. J. M. Vriesema, and K. van Norren Carbohydrate Supplementation Before Operation Retains Intestinal Barrier Function and Lowers Bacterial Translocation in a Rat Model of Major Abdominal Surgery JPEN J Parenter Enteral Nutr, May 1, 2008; 32(3): 247 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Newman, P. Yang, A. D. Pawlus, and K. I. Block Cardiac Glycosides as Novel Cancer Therapeutic Agents Mol. Interv., February 1, 2008; 8(1): 36 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Vagin, E. Tokhtaeva, I. Yakubov, E. Shevchenko, and G. Sachs Inverse Correlation between the Extent of N-Glycan Branching and Intercellular Adhesion in Epithelia: CONTRIBUTION OF THE Na,K-ATPase 1 SUBUNIT J. Biol. Chem., January 25, 2008; 283(4): 2192 - 2202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Lubarski, S. J. D. Karlish, and H. Garty Structural and functional interactions between FXYD5 and the Na+-K+-ATPase Am J Physiol Renal Physiol, December 1, 2007; 293(6): F1818 - F1826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Laughery, R. J. Clifford, Y. Chi, and J. H. Kaplan Selective basolateral localization of overexpressed Na-K-ATPase beta1- and beta2- subunits is disrupted by butryate treatment of MDCK cells Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1718 - F1725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Madan, K. Rose, and A. J. Watson Na/K-ATPase beta1 Subunit Expression Is Required for Blastocyst Formation and Normal Assembly of Trophectoderm Tight Junction-associated Proteins J. Biol. Chem., April 20, 2007; 282(16): 12127 - 12134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Cibrian-Uhalte, A. Langenbacher, X. Shu, J.-N. Chen, and S. Abdelilah-Seyfried Involvement of zebrafish Na+,K+ ATPase in myocardial cell junction maintenance J. Cell Biol., January 16, 2007; 176(2): 223 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Paul, M. J. Palladino, and G. J. Beitel A pump-independent function of the Na,K-ATPase is required for epithelial junction function and tracheal tube-size control Development, January 1, 2007; 134(1): 147 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Rajasekaran, S. P. Barwe, J. Gopal, S. Ryazantsev, E. E. Schneeberger, and A. K. Rajasekaran Na-K-ATPase regulates tight junction permeability through occludin phosphorylation in pancreatic epithelial cells Am J Physiol Gastrointest Liver Physiol, January 1, 2007; 292(1): G124 - G133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Vagin, E. Tokhtaeva, and G. Sachs The Role of the beta1 Subunit of the Na,K-ATPase and Its Glycosylation in Cell-Cell Adhesion J. Biol. Chem., December 22, 2006; 281(51): 39573 - 39587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Christiansen and A. K. Rajasekaran Reassessing Epithelial to Mesenchymal Transition as a Prerequisite for Carcinoma Invasion and Metastasis. Cancer Res., September 1, 2006; 66(17): 8319 - 8326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liang, T. Cai, J. Tian, W. Qu, and Z.-J. Xie Functional Characterization of Src-interacting Na/K-ATPase Using RNA Interference Assay J. Biol. Chem., July 14, 2006; 281(28): 19709 - 19719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Toyama, Z. Zhang, H. Iwase, H. Yamashita, Y. Ando, M. Hamaguchi, M. Mizutani, N. Kondo, T. Fujita, Y. Fujii, et al. Low Expression of the Snail Gene is a Good Prognostic Factor in Node-Negative Invasive Ductal Carcinomas Jpn. J. Clin. Oncol., June 1, 2006; 36(6): 357 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mazzanti, M. Solazzo, O. Fantappie, S. Elfering, P. Pantaleo, P. Bechi, F. Cianchi, A. Ettl, and C. Giulivi Differential expression proteomics of human colon cancer Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1329 - G1338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Comellas, L. A. Dada, E. Lecuona, L. M. Pesce, N. S. Chandel, N. Quesada, G. R. S. Budinger, G. J. Strous, A. Ciechanover, and J. I. Sznajder Hypoxia-Mediated Degradation of Na,K-ATPase via Mitochondrial Reactive Oxygen Species and the Ubiquitin-Conjugating System Circ. Res., May 26, 2006; 98(10): 1314 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-N. Lian, T.-W. Wu, R.-L. Dao, Y.-J. Chen, and C.-H. Lin Deglycosylation of Na+/K+-ATPase causes the basolateral protein to undergo apical targeting in polarized hepatic cells J. Cell Sci., January 1, 2006; 119(1): 11 - 22. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Kaplan A Moving New Role for the Sodium Pump in Epithelial Cells and Carcinomas Sci. Signal., June 21, 2005; 2005(289): pe31 - pe31. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chifflet, J. A. Hernandez, and S. Grasso A possible role for membrane depolarization in epithelial wound healing Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1420 - C1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Christiansen, S. A. Rajasekaran, L. Inge, L. Cheng, G. Anilkumar, N. H. Bander, and A. K. Rajasekaran N-glycosylation and microtubule integrity are involved in apical targeting of prostate-specific membrane antigen: implications for immunotherapy Mol. Cancer Ther., May 1, 2005; 4(5): 704 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Barwe, G. Anilkumar, S. Y. Moon, Y. Zheng, J. P. Whitelegge, S. A. Rajasekaran, and A. K. Rajasekaran Novel Role for Na,K-ATPase in Phosphatidylinositol 3-Kinase Signaling and Suppression of Cell Motility Mol. Biol. Cell, March 1, 2005; 16(3): 1082 - 1094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Shoshani, R. G. Contreras, M. L. Roldan, J. Moreno, A. Lazaro, M. S. Balda, K. Matter, and M. Cereijido The Polarized Expression of Na+,K+-ATPase in Epithelia Depends on the Association between {beta}-Subunits Located in Neighboring Cells Mol. Biol. Cell, March 1, 2005; 16(3): 1071 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Greenough, L. Pase, I. Voskoboinik, M. J. Petris, A. W. O'Brien, and J. Camakaris Signals regulating trafficking of Menkes (MNK; ATP7A) copper-translocating P-type ATPase in polarized MDCK cells Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1463 - C1471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Espineda, J. H. Chang, J. Twiss, S. A. Rajasekaran, and A. K. Rajasekaran Repression of Na,K-ATPase {beta}1-Subunit by the Transcription Factor Snail in Carcinoma Mol. Biol. Cell, March 1, 2004; 15(3): 1364 - 1373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Rajasekaran and S. A. Rajasekaran Role of Na-K-ATPase in the assembly of tight junctions Am J Physiol Renal Physiol, September 1, 2003; 285(3): F388 - F396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. SASSON, A. DANTES, K. TAJIMA, and A. AMSTERDAM Novel genes modulated by FSH in normal and immortalized FSH-responsive cells: new insights into the mechanism of FSH action FASEB J, July 1, 2003; 17(10): 1256 - 1266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Genova and R. G. Fehon Neuroglian, Gliotactin, and the Na+/K+ ATPase are essential for septate junction function in Drosophila J. Cell Biol., June 9, 2003; 161(5): 979 - 989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Rajasekaran, J. Hu, J. Gopal, R. Gallemore, S. Ryazantsev, D. Bok, and A. K. Rajasekaran Na,K-ATPase inhibition alters tight junction structure and permeability in human retinal pigment epithelial cells Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1497 - C1507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Anilkumar, S. A. Rajasekaran, S. Wang, O. Hankinson, N. H. Bander, and A. K. Rajasekaran Prostate-specific Membrane Antigen Association with Filamin A Modulates Its Internalization and NAALADase Activity Cancer Res., May 15, 2003; 63(10): 2645 - 2648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Rajasekaran, L. G. Palmer, S. Y. Moon, A. Peralta Soler, G. L. Apodaca, J. F. Harper, Y. Zheng, and A. K. Rajasekaran Na,K-ATPase Activity Is Required for Formation of Tight Junctions, Desmosomes, and Induction of Polarity in Epithelial Cells Mol. Biol. Cell, December 1, 2001; 12(12): 3717 - 3732. [Abstract] [Full Text] [PDF]
|
|
