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Vol. 12, Issue 2, 383-392, February 2001

The Cellular Level of PR500, a Protein Complex Related to the 19S Regulatory Particle of the Proteasome, Is Regulated in Response to Stresses in Plants

Zhaohua Peng,* Jeffrey M. Staub,*dagger Giovanna Serino,* Shing F. Kwok,*Dagger Jasmina Kurepa,§ Barry D. Bruce,|| Richard D. Vierstra,§ Ning Wei,* and Xing-Wang Deng*

 *Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104;  ||Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee at Knoxville, Knoxville, Tennessee 37996; and  §Cellular and Molecular Biology Program, University of Wisconsin-Madison, Madison, Wisconsin 53706

In Arabidopsis seedlings and cauliflower florets, Rpn6 (a proteasome non-ATPase regulatory subunit) was found in two distinct protein complexes of ~800 and 500 kDa, respectively. The large complex likely represents the proteasome 19S regulator particle (RP) because it displays the expected subunit composition and all characteristics. The small complex, designated PR500, shares at least three subunits with the "lid" subcomplex of 19S RP and is loosely associated with an hsp70 protein. In Arabidopsis COP9 signalosome mutants, PR500 was specifically absent or reduced to an extent that correlates with the severity of the mutations. Furthermore, PR500 was also diminished in response to potential protein-misfolding stresses caused by the heat shock and canavanine treatment. Immunofluorescence studies suggest that PR500 has a distinct localization pattern and is enriched in specific nuclear foci. We propose that PR500 may be evolved in higher plants to cope with the frequently encountered environmental stresses.

Present addresses: dagger Monsanto Company, 700 Chesterfield Parkway North, Mail Code BB3G, St. Louis, MO 63017; Dagger Ceres, Inc., 3007 Malibu Canyon Road, Malibu, CA 90265.

Corresponding author. E-mail address: xingwang.deng{at}yale.edu.

Molecular Biology of the Cell
Vol. 12, 383-392, February 2001
Copyright © 2001 by The American Society for Cell Biology


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